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  • 1
    Electronic Resource
    Electronic Resource
    Bacterial Studies on Irradiated Tropical Fish—Bombay Duck (Harpodon nehereus) (1967)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 32 (1967), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: SUMMARY— Gamma irradiation (0.4 Mrad) of Bombay duck (Havpodon nehereus) fillets sealed in polyethylene bags brought about alterations in the bacterial flora during storage at 10–12°C. Micrococcus, Pseudomondas, Ackromobacter, Flavobacterium, Microbacterium, Bacillus, Alkaligenes, and Sarcina, which comprised the initial flora of Bombay duck, were predominated by Proteus, Bacillus, Aeromonas, Micro-cocci, and Neisseria in the spoiled samples after four days storage at 10–12°C. In contrast, absence of putrid odors in the irradiated samples stored at 10–12°C for 14 days may be attributed to the biochemically inactive nature of the predominating Micrococci.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.1967.tb00871.x
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  • 2
    Electronic Resource
    Electronic Resource
    Liposomes as vehicle for allergen presentation in the immunotherapy of allergic diseases (1991)
    Oxford, UK : Blackwell Publishing Ltd
    Allergy 46 (1991), S. 0 
    Details
    ISSN: 1398-9995
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Liposomes are non-toxic, biodegradable and weakly immunogenic lipid vesicles which can be used as immunomodulating agents. In the present study, multilamellar vesicles (MLV) and small unilamellar vesicles (SUV) were used to incorporate an allergenic protein from Artemisia scoparia pollen. MLV incorporated more allergenic protein than SUV. To assess the immunomodulating effect of allergen entrapped in liposomes, Swiss strain mice (made IgE responders) were injected with either free allergen or liposome-entrapped allergen (LEA) and their immune response was measured in terms of specific IgG and specific IgE levels. Results indicated that specific IgE response was significantly lower in mice injected LEA (P 〈 0.02) than in mice injected free allergenic protein. Although specific IgG response was higher in mice injected LEA, there was no statistically significant difference between the two groups. Potential use of liposomes as non-immunogenic biocompatible vehicle for antigen presentation in immunotherapy will be discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1398-9995.1991.tb00603.x
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  • 3
    Electronic Resource
    Electronic Resource
    Fusarium solani major allergen peptide IV-1 binds IgE but does not release histamine (2001)
    Oxford, UK : Blackwell Science, Ltd
    Clinical & experimental allergy 31 (2001), S. 0 
    Details
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background Fusarium solani (FS) is an important allergen source afflicting 4% of the nasobronchial allergy patients. Fus s I3596*, a 65 kDa major glycoprotein allergen of FS reacts with 95% fungus sensitive patients.Objectives To purify and characterize a potent peptide from Fus s I3596* which may be useful for therapeutic purposes.Methods The 65 kDa protein was sequentially cleaved with trypsin and cyanogen bromide (CNBr). The cleaved products were purified on reverse phase high performance liquid chromatography (rpHPLC) column and functionally characterized by in vitro and in vivo methods for its IgE binding and histamine release.Results The protein on cleavage showed 11 peaks (I to XI). Of these, peaks I, III, IV and V were highly allergenic as determined by IgE ELISA. These peaks were further purified and peptide IV-1 was most potent in comparison to other peptides by ELISA-inhibition. This peptide showed IgE binding but could not evoke intradermal response in Fusarium-sensitive patients. Heparinized blood challenged with peptide IV-1 does not release histamine. Preincubation of heparinized blood with peptide IV-1 and challenging with crude extract blocked histamine release in a dose dependent manner.Conclusion Peptide IV-1 binds to IgE but does not release histamine, demonstrating its potential use in therapy of Fusarium-allergic patients.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2222.2001.01080.x
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  • 4
    Electronic Resource
    Electronic Resource
    Efficacy of liposome entrapped allergen in down regulation of IgE response in mice (1992)
    Oxford, UK : Blackwell Publishing Ltd
    Clinical & experimental allergy 22 (1992), S. 0 
    Details
    ISSN: 1365-2222
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The effect of liposome entrapped allergen on primary, secondary and ongoing IgE response was studied in mice. It was observed that mice primed with liposome entrapped allergen maintained significantly lower levels of serum specific IgE as compared to that of controls primed with allergen. Although alum adsorbed allergen could induce IgE synthesis in mice primed with liposome entrapped allergen the increase in serum specific IgE levels was lower than the animals primed and challenged with alum adsorbed allergen. On the contrary when BALB/c mice were primed with alum adsorbed allergen to induce IgE synthesis subsequent challenge by liposome entrapped allergen could down regulate the serum specific IgE response. Serum specific IgG response did not show any significant difference between the two groups. There was an increase in T suppressor cell subpopulation as measured by immunofluoresence technique in mice injected liposome entrapped allergen as compared to that in controls. The results indicate that liposome entrapped allergens may prove to be safe and effective in immunotherapy by reducing the allergenic response of the allergen at the same time increasing the antigenicity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2222.1992.tb00112.x
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  • 5
    Electronic Resource
    Electronic Resource
    RADIATION PASTEURIZATION OF FRESH AND BLANCHED TROPICAL SHRIMPS (1970)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 35 (1970), S. 0 
    Details
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: SUMMARY— The storage life of fresh, peeled and deveined tropical shrimps at 10—12°C is extended to 10—14 and 18—21 days with 0.15 and 0.25 Mrad, respectively, as against spoilage of unirradiated samples within 4 days. Blanching treatment (steaming for 4 min) with or without brine (3.0% NaCl) extends the storage life up to 30 days at lo—12°C. the former retaining the shell-fish flavor. However, the blanched products develop musty odor and slimy texture during storage; subjected to irradiation (0.15 Mrad) these are devoid of undesirable attributes and are acceptable for 60 and 130 days, respectively, at 10—1°C and 2—4°C. Bacterial counts exceeding 108/g and high levels of TMAN and TVBN concur with spoilage of unirradiated shrimps. In contrast, low levels of TMAN and TVBN, despite high TBC, corroborate with high acceptability of radiation processed shrimps. In blanched as well as in blanched and irradiated shrimps TBC, TMAN and TVBN are low. Samples subjected to blanching only have low acceptability as against the improvement in quality by irradiation. These alterations in storage property have been discussed in the light of shifts in microflora.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2621.1970.tb00930.x
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  • 6
    Electronic Resource
    Electronic Resource
    Immunocheraical investigation of allergens from Rhizopus nigricans (1990)
    Oxford, UK : Blackwell Publishing Ltd
    Allergy 45 (1990), S. 0 
    Details
    ISSN: 1398-9995
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The allergenic proteins of mould, Rhizopus nigricans extract (RNE) were identified and characterized by crossed immunoelectrophoresis (CIE), thin-layer isoelectrofocusing (TLIEF) and RAST inhibition. CIE revealed that the extract contained at least 31 distinct antigens. On TLIEF the extract resolved into 22 bands in pi 3.5–6.8. Two important allergens, Rhiz IIIb and VIb were purified by a combination of ammonium sulfate precipitation, anion exchange chromatography on DEAE-Sephadex column and gel filtration. Twenty and 12 μg of Rhiz IIIb and Rhiz VIb were sufficient to give 50% RAST inhibition as against 43 μg of crude RNE. Rhiz IIIb and Rhiz VIb were found to be glycoproteins with molecular weights of 12,400 daltons and 14,200 daltons, respectively. Rhiz IIIb was found to be homogeneous on polyacrylamide gel electrophoresis (PAGE) and TLIEF with a pI of 4.8, while Rhiz VIb gave a single band on PAGE and resolved into two Coomassie blue stained bands with pI 3.6 and 3.8. It was possible to separate the components of RNE on fast protein liquid chromatography (FPLC) using an anion exchanger Mono Q column. The identification and characterization of the antigenic and allergenic proteins in the extract will be useful in standardization of RNE and in preparation of an in-house reference standard.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1398-9995.1990.tb00943.x
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  • 7
    Electronic Resource
    Electronic Resource
    Head-high, airborne pollen grains from different areas of metropolitan Delhi (1990)
    Oxford, UK : Blackwell Publishing Ltd
    Allergy 45 (1990), S. 0 
    Details
    ISSN: 1398-9995
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: A survey of airborne pollen grains from four zones of metropolitan Delhi was conducted for 1 year (February 1988-January 1989) at human height level (5′-6′). Sampling was carried out in different inhabited areas in the four zones using Burkard Volumetric Personal Samplers. Sampling was carried out at weekly intervals, three times a day (7, 14, & 20 h) for 15 min. Poaceae, Ricinus, Cheno-Amaranth, Moms, Artemisia, Myrtaceae, Parthenium, Prosopis and Cannabis are important pollen contributors to the atmosphere, especially at lower heights. In general, pollen concentration was low at human height. Quantitative zonal variations have been recorded within an urban city.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1398-9995.1990.tb00499.x
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  • 8
    Electronic Resource
    Electronic Resource
    Isolation and immunobiochemical characterization of a major allergen (65 kDa) from Fusarium equiseti (1998)
    Oxford, UK : Blackwell Publishing Ltd
    Allergy 53 (1998), S. 0 
    Details
    ISSN: 1398-9995
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Fusarium equiseti is one of the most important species in the class Deuteromycetes (Fungi Imperfecti). For proper diagnosis and immunotherapy, isolation and characterization of allergens of F. equiseti are necessary. In the present study, culture filtrate (CF) extract of F. equiseti was resolved into 35–37 bands on isoelectric focusing pi (3–9) and SDS-PAGE (mol. wt. 10–100 kDa). Most of them were glycoproteins, as identified by PAS staining. F. equiseti CF revealed 15 allergenic proteins on immunoblot with an allergic serum pool. It was fractionated into nine fractions (I–IX) on a Superose-12 column by FPLC. Fraction IV (65 kDa) and fraction VI (25 kDa) were found to be highly allergenic by IgE ELISA. A 65-kDa protein was observed as a major allergen because it was recognized by most of the patient sera on immunoblot. After elution from SDS-PAGE gel, it gave two bands of pi 7.4 and 6.0. Inhibition in IgE-binding components of F. equiseti CF with CF extracts of F. soiani and F. moniliforme by immunoprint inhibition assay indicated the allergenicity shared between the extracts of Fusarium species. Data suggested that the 65-kDa is the major allergen in the Fusarium species and can he used for the treatment of allergic patients.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1398-9995.1998.tb03893.x
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  • 9
    Electronic Resource
    Electronic Resource
    Studies on shared antigenic/allergenic components among fungi (1995)
    Oxford, UK : Blackwell Publishing Ltd
    Allergy 50 (1995), S. 0 
    Details
    ISSN: 1398-9995
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Fungal allergens have been found to be one of the most prevalent aeroallergens in India. Knowledge of shared/unique components among different fungi is necessary for proper diagnosis and treatment of patients allergic to fungi. In the present study, crude extracts (CE) of 11 common fungi (Alternaria alternata, Aspergillus flavus, Asp. fumigatus, Asp. niger, Asp. tamarii, Asp. versicolor, Cladosporium herbarum, Curvularia lunata, Mucor hiemalis, Penicillium citrinum, and Fusarium solani) were characterized by isoelectric focusing (IEF), SDS-PAGE, and immunoblot. On IEF (pI 3–9), the number of protein bands was found to be greatest (46) in M. hiemalis extract. SDS-PAGE exhibited a varied number of bands, generally 18–40, with mol. mass ranging from 14 to 100 kDa. IgG-specific immunoprint using rabbit anti-F. solani CF antibodies demonstrated a mol. mass distribution of shared antigenic proteins of 14–100 kDa in most of the fungi. Shared allergenicity was observed in a number of allergenic proteins in fungal extracts with mol. mass ranging between 14 and 70 kDa on IgE-specific immunoblot using pooled sera of patients allergic to Fusarium. A 45-kDa protein was found to be common among these fungi on immunoblot with patients as well as with rabbit antibodies. F. solani CF extract contained more antigenic/allergenic proteins than F. solani CE. It was concluded that F. solani CF shared several antigenic/allergenic components with CE of other common fungi. This fact needs to be taken into account when fungal extracts are used in diagnosis and immunotherapy of allergic patients.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1398-9995.1995.tb05054.x
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  • 10
    Electronic Resource
    Electronic Resource
    Studies on Fusarium solani (1994)
    Oxford, UK : Blackwell Publishing Ltd
    Allergy 49 (1994), S. 0 
    Details
    ISSN: 1398-9995
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Allergenic cross-reactivity among three Fusarium species (F. solani, F. equiseti, and F. moniliforme) was determined by crossed immunoelectrophoresis (CIE), ELISA inhibition, and immunoprint-inhibition analysis. Inhibiting immunoprint was used to assess the cross-reactivity of individual allergenic proteins in culture filtrate (CF), separated on SDS-PAGE. Dose-response inhibition of band 14 kDa in immunoprints of F. solani CF by antigens of F. solani CF, F. equiseti CF, and F. moniliforme CF demonstrated significant cross-reactivity of allergenic proteins on analysis of densitometric scans. CIE and ELISA inhibition using hyperimmune sera raised in rabbits against F. solani CF antigen also demonstrated shared antigenicity among Fusarium species. Collectively, this study revealed the presence of shared as well as unique antigenic and allergenic determinants in F. solani CF, F. equiseti CF, and F. moniliforme CF antigens.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1398-9995.1994.tb02277.x
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