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  • 1
    Electronic Resource
    Electronic Resource
    MAP kinase-related FUS3 from S. cerevisiae is activated by STE7 in vitro (1993)
    [s.l.] : Nature Publishing Group
    Nature 362 (1993), S. 261-264 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] To study FUS3 phosphorylation in vitro we isolated it as a Myc epitope-tagged fusion protein from Escherichia coli (Fig. la). We expressed and isolated two inactive mutant derivatives of FUSS in a similar way. One has a substitution at the conserved catalytic lysine (FUS3M-R42) and the other has ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/362261a0
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Alfalfa heat shock genes are differentially expressed during somatic embryogenesis (1991)
    Springer
    Plant molecular biology 16 (1991), S. 999-1007 
    Details
    ISSN: 1573-5028
    Keywords: Medicago sativa L. ; stress response ; tissue culture ; somatic embryogenesis ; heat shock protein (HSP)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have isolated two cDNA clones (Mshsp18-1; Mshsp18-2) from alfalfa (Medicago sativa L.) which encode for small heat shock proteins (HSPs) belonging to the hsp17 subfamily. The predicted amino acid sequences of the two alfalfa proteins are 92% identical and a similar degree of homology (90%) can be detected between Mshsp 18-2 and the pea hsp 17. In comparison to various members of small HSPs from soybean amino acid sequence similarities of 80–86% were identified. The alfalfa HSPs share a homologous stretch of amino acids in the carboxy terminal region with hsp22, 23, 26 from Drosophila. This region contains the GVLTV motif which is characteristic of several members of small HSPs. At room temperature alfalfa hsp 18 mRNAs were not detectable in root and leaf tissues but northern analysis showed a low level of expression in microcallus suspension (MCS). The transcription of Mshsp 18 genes is induced by elevated temperature, CdCl2 treatment and osmotic shock in cultured cells. In alfalfa somatic embryos derived from MCS a considerable amount of hsp 18 mRNA can be detected during the early embryogenic stages under normal culture conditions. The differential expression of these genes during embryo development suggests a specific functional role for HSPs in plant cells at the time of the developmental switch in vitro.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00016072
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  • 3
    Electronic Resource
    Electronic Resource
    Isolation and characterization of a tobacco cDNA clone encoding a putative MAP kinase (1993)
    Springer
    Plant molecular biology 23 (1993), S. 543-551 
    Details
    ISSN: 1573-5028
    Keywords: Cell cycle ; MAP kinases ; Nicotiana tabacum ; pollen development ; pollen embryogenesis ; starvation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have isolated and sequenced a MAP (mitogen-activated protein) kinase-type cDNA from a tobacco (Nicotiana tabacum L.) cell suspension cDNA library by screening with a PCR fragment amplified from the same library with oligonucleotide primers corresponding to two sequences conserved in yeast and animal MAP kinases. The tobacco sequence, ntf3, shows 45–54% identity to various members of the MAP kinase family at the protein level. Northern experiments showed that ntf3 is expressed in all tobacco tissues tested, including pollen isolated at different developmental stages. Southern analysis indicated that, as in other organisms, there is a family of MAP kinase genes in tobacco. In complementary tests, ntf3 could not substitute the yeast MAP kinase genes fus3 and kss1.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00019302
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