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  • 1
    Electronic Resource
    Electronic Resource
    Distribution of collagens type V and VI in the normal human alveolar mucosa: An immunoelectronmicroscopic study using ultrathin frozen sections (1991)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of periodontal research 26 (1991), S. 0 
    Details
    ISSN: 1600-0765
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The ultrastructural localization of collagens type V and VI in normal human gingival mucosa was investigated by immunoelectron microscopy. Twenty biopsies were fixed in dimethylsuberimidate and shock-frozen in slush nitrogen. Collagen type V was mainly located to meshworks of uniform nonstriated microfibrils of 12 to 20 nm width, which preferentially appeared in larger spaces between cross-striated major collagen fibrils. Occasionally single microfibrils of collagen type V fanned out from the ends of major collagen fibrils, which may indicate a role as a core fibril. Collagen type V was not found in the subepithelial basement membrane and the immediately adjacent stroma. Collagen type VI was detected in a loose reticular network of unhanded microfilaments that were morphologically distinguishable by knoblike protrusions every 100–110 nm. These microfilaments were found in the vicinity, but not as an intrinsic component, of the subepithelial basement membrane. Single filaments of collagen type VI filaments appeared to form bridges between neighboring cross-striated major collagen fibrils, suggesting an interconnecting role for this collagen type. The method presented appears to be excellently suited to study the normal and pathological supramolecular organization of the oral extracellular matrix.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0765.1991.tb01637.x
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  • 2
    Electronic Resource
    Electronic Resource
    Epstein-Barr virus in lingual epithelium of liver transplant patients (1993)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of oral pathology & medicine 22 (1993), S. 0 
    Details
    ISSN: 1600-0714
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Thirty-three liver transplant patients and 20 controls were examined for the presence of EBV in epithelium of the lateral border of the tongue by negative staining electron microscopy. Five of the specimens (15%) showed particles typical of the herpes virus family, while all controls were negative for EBV. In 3 of the 5 patients moderate oral hairy leukoplakia was observed clinically. Our results indicate that EBV may be expressed at the lateral border of the tongue of liver transplant patients, who in some cases show oral hairy leukoplakia clinically.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0714.1993.tb01070.x
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  • 3
    Electronic Resource
    Electronic Resource
    Negative staining EM for the detection of Epstein-Barr virus in oral hairy leukoplakia (1990)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of oral pathology & medicine 19 (1990), S. 0 
    Details
    ISSN: 1600-0714
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The performance of two different EM techniques applied for the detection of Epstein-Barr Virus (EBV) in oral hairy leukoplakia (HL) was assessed, i.e. the conventional two-step method of negative staining (CNS) and negative staining after Airfuge enrichment (ANS). Scrape specimens from the lateral borders of tongue of 66 HIV-positive patients with or without HL, of 3 patients with infectious mononucleosis and of 10 HIV-negative patients were evaluated. While CNS resulted in virus detection only in 25% of clinically diagnosed HL cases, EBV was detected by ANS in 85% of clinically suspected cases of HL. Scrape specimens of individuals negative for HIV were negative in EM while 2 of 3 mononucleosis patients were positive without clinical evidence for HL. Due to this high sensitivity the method of negative staining after Airfuge enrichment appears to be useful in the diagnosis of HL. The finding of EBV in clinically normal oral mucosa in HIV-seropositive individuals is interesting and indicates that EBV expression may precede the clinical appearance of HL.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0714.1990.tb00861.x
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  • 4
    Electronic Resource
    Electronic Resource
    Ultrastructural findings in oral Kaposi's sarcoma (AIDS) (1987)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of oral pathology & medicine 16 (1987), S. 0 
    Details
    ISSN: 1600-0714
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Ten biopsies of oral Kaposi's sarcoma were examined histologically and ultrastructurally. Histologically, early and late tumor stages could be differentiated. On the Ultrastructural level, endothelial-like and spindle-shaped tumor cells were revealed. Tumor cells associated with vessel-like spaces showed partly interrupted basal membranes, Weibel-Palade bodies, desmosomes and tight-junctions, while spindle-shaped cells lacked these Ultrastructural features of endothelial cells. Within the cytoplasm of endothelial-like cells, aggregates of tubular structures were observed. Histological and Ultrastructural findings in oral Kaposi sarcoma are comparable to those of such other organs as skin and intestines. The Ultrastructural findings indicate an endothelial origin, at least of endothelial-like tumor cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1600-0714.1987.tb00711.x
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  • 5
    Electronic Resource
    Electronic Resource
    Immunoelectron microscopy shows an atypical pattern and a quantitative shift of collagens type I, III and VI in oral Kaposi's sarcoma of AIDS (1991)
    Springer
    Virchows Archiv 419 (1991), S. 237-244 
    Details
    ISSN: 1432-2307
    Keywords: Collagen ; Immunoelectron microscopy ; Immunocryoultramicrotomy ; Kaposi's sarcoma ; AIDS
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The localization of collagen types I, III and VI in normal human alveolar and palatal mucosa and in oral Kaposi's sarcoma (KS) was studied by light microscopy and cryo-immunoelectron microscopy. Normal oral mucosa revealed two different types of organization. The upper connective tissue stroma contained a loose reticular network mainly composed of collagen types III and VI, while collagen type I immunostaining predominated in the deeper stroma. Ultrastructurally, in the KS tumour stroma, a loose pattern of individual thin collagen fibrils was noted. These often fanned out at their ends showing a filamentous substructure. The fibrils consisted predominantly of collagen type I similar to individual fibrils of normal oral mucosa. However, there was a marked loss of thick fibre bundles of collagen types I and III in KS compared with normal oral mucosa, whereas collagen type VI was markedly increased and found preferentially in clusters and strands around cross-striated fibrils that often spanned the distance between single collagen fibres. The abundance of collagen type VI in a pattern similar to early stages of wound healing suggests that the KS stroma resembles an early organizational stage of the interstitial and vascular extracellular matrix subject to a high rate of collagen turnover. This character of the KS stroma appears to result from a continuous auto- and paracrine stimulation of cell growth and collagen synthesis and provides an excellent model to study the structural arrangement of collagen type VI in relation to the fibrillar collagen types I and III.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01626354
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  • 6
    Electronic Resource
    Electronic Resource
    Morphogenesis and morphology of HIV structure-function relations (1989)
    Springer
    Archives of virology 106 (1989), S. 1-13 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Fine structure and antigenic make-up analysis of HIV were combined in a 2D model, from which functional aspects can be deduced. On the envelope 72 probably trimeric surface knobs (gp 120) are connected to the virion via the transmembrane protein gp41. Gp120 is shed during ageing of the virion, but host cell antigens stay firmly anchored to the envelope. Underneath the envelope, p17 forms the matrix protein layer, while the capsid of the double cone shaped core is built up of p24. The relation between biochemical findings and morphogensis and maturation of HIV as well as aspects of pathogenesis and vaccination are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01311033
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  • 7
    Electronic Resource
    Electronic Resource
    The organization of the envelope projections on the surface of HIV (1988)
    Springer
    Archives of virology 100 (1988), S. 255-266 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The organization of envelope projections (knobs) of four different isolates of the human immunodeficiency virus types 1 and 2 (HIV-1 and -2) was studied using surface replica and thin section electron microscopy (EM) in combination with rotational image enhancement. All HIV strains show an identical organization of knobs on the virus envelope. The surface of an “ideal”, well-preserved HIV particle is studded with 72 knobs arranged in a T=7 laevo symmetry. The role of the p17 protein, which is coating the inner leaflet of the viral envelope, is discussed as a matrix protein functioning as a scaffold for the envelope and its projections during morphogenesis as well as with mature virions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01487688
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  • 8
    Electronic Resource
    Electronic Resource
    Compartment-specific immunolocalization of conserved epitopes of the glycoprotein gB of herpes simplex virus type 1 and bovine herpes virus type 2 in infected cells (1989)
    Springer
    Archives of virology 108 (1989), S. 1-17 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Monoclonal antibodies directed against a surface glycoprotein of the bovine herpes virus type 2 (BHV-2, bovine herpes mammillitis virus) recognize also determinants of the major glycoprotein gB of the human herpes simplex virus type 1 (HSV-1). Cross-reacting antigens of the virions and in infected cells were localized with immunocytochemical methods, immunofluorescence as well as pre-embedding and cryoultramicrotomy immune electron microscopy. All antibodies stain to different degrees cell free BHV-2 and HSV-1 virions. In the cell two predominant staining patterns could be observed indicating that expression of epitopes is dependent upon the cell compartment: (i) staining of cytoplasmic membranes and enveloped particles within membrane systems and (ii) staining of intranuclear antigens. Antibodies tagging intranuclear antigens react with moderately dense material or with the periphery of nucleocapsids. This unexpected result is interpreted in terms of two hypotheses: (1) presence of common epitopes on two entirely different herpesvirus proteins conserved in HSV-1 and BHV-2 and (2) transport of gB or its precursor into the nucleus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01313738
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  • 9
    Electronic Resource
    Electronic Resource
    Conserved epitopes of simian herpesvirus SA 8 and bovine herpesvirus type 2 (1990)
    Springer
    Archives of virology 111 (1990), S. 1-14 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Some major structural components of simian herpesvirus SA 8 were analyzed and the relationship of SA 8 with HSV-1 and especially with BHV-2 was further characterized using a panel of SA 8- and BHV-2-specific monoclonal antibodies directed against gB, gD, gE, and ICP 8. It could be shown that SA 8 and BHV-2 expressed gB-1 equivalents, which differ in electrophoretic mobility, but share common epitopes with HSV-1. The antigenic determinants were detectable in the cytoplasm, on the surface of infected cells and on the virus envelope. Monoclonal antibodies reactive with epitopes of gB-SA 8 and gB-BHV-2 neutralized the homologous virus and cross-neutralized only HSV-1 and HSV-2, suggesting differences in accessability of the corresponding epitope on SA 8 and BHV-2, respectively. A second protein with conserved epitopes on SA 8, BHV-2, and HSV-1 was identified as ICP 8. This nucleus associated protein was additionally detected on the envelope of SA 8 and HSV-1. The results imply that ICP 8 might have a function not only in virus replication, but also in virus assembly. We could furthermore define type-specific epitopes on two SA 8 envelope proteins which are analogous to gD-1 and gE-1, respectively. The gD-specific epitope induced a type-specific neutralizing antibody, making it interesting for differentiation of closely related herpesviruses.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01310501
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  • 10
    Electronic Resource
    Electronic Resource
    Analysis of non-infectious HIV particles produced in presence of HIV proteinase inhibitor (1991)
    Springer
    Archives of virology 120 (1991), S. 71-81 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Newly developed substrate analogue peptidomimetics are able to inhibit the human immunodeficiency virus, HIV-1 proteinase at nanomolar concentration. In HIV infected cell culture they exhibit antiviral activity. We have analyzed the non-infectious HIV particles produced in chronically HIV infected cell culture in presence of one of these inhibitors. The total production of virus particles was not substantially reduced in drug treated cultures, compared to non-inhibited control cultures, but the infectivity of these virus particles was reduced about 100 fold. The processing ofgag andgag-pol protein precursor was inhibited; only borderline activity of reverse transcriptase (RT) could be detected in these particles and they contained nonprocessedgag precursor protein. Thin section electron microscopy of inhibitor-treated, HIV-infected cells revealed reduced viral cytopathogenicity and both inhibition of particle assembly and incomplete maturation of the particles formed. The HIV particles produced in the presence of the proteinase inhibitor were studded with envelope glycoprotein knobs and often comprised multiple budding regions, but were morphologically immature.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01310950
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