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  • 1
    ISSN: 1432-2242
    Keywords: Hessian fly resistance ; Insect antibiosis ; Wheat-rye hybrids ; C banding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A new Hessian fly (Mayetiola destructor) resistance gene derived from ‘Balbo’ rye and its transfer to hexaploid wheat via radiation-induced terminal and intercalary chromosomal translocations are described. Crosses between resistant ‘Balbo’ rye and susceptible ‘Suwon 92’ wheat and between the F1 amphidiploids and susceptible ‘TAM 106’ and ‘Amigo’ wheats produced resistant BC2F3 lines that were identified by C-banding analysis as being 6RL telocentric addition lines. Comparative chromosomal analyses and resistance tests revealed that the resistance gene is located on the 6RL telocentric chromosome. X-irradiated pollen of 6RL addition plants was used to fertilize plants of susceptible wheats ‘TAM 106,’ ‘TAM 101,’ and ‘Vona.’ After several generations of selection for resistance, new sublines were obtained that were homogeneous for resistance. Thirteen of these lines were analyzed by C-banding, and three different wheat-6RL chromosomal translocations (T) were identified. Wheat chromosomes involved in the translocations were 6B, 4B, and 4A. Almost the complete 6RL arm is present in T6BS · 6BL-6RL. Only the distal half of 6RL is present in T4BS · 4BL-6RL, which locates the resistance gene in the distal half of 6RL. Only a very small segment (ca 1.0 μm) of the distal region of 6RL is present in an intercalary translocation (Ti) Ti4AS · 4AL-6RL-4AL. The 6RL segment is inserted in the intercalary region between the centromere of chromosome 4A and the large proximal C-band of 4AL. The break-points of the translocations are outside the region of the centromere, indicating that they were induced by the X-ray treatment. All three translocations are cytologically stable and can be used directly in wheat breeding programs.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Radiation-induced wheat-rye chromosome translocation lines resistant to Hessian fly, Mayetiola destructor (say), were analyzed by in situ hybridization using total genomic and highly repetitive rye DNA probes pSc119 and pSc74. In situ hybridization analysis revealed the exact locations of the translocation breakpoints and allowed the estimation of the sizes of the transferred rye segments. T6BS·6BL-6RL and T4BS· 4BL-6RL are terminal translocations with either most of the complete long arm of rye chromosome 6R or only the distal 57% of the 6RL arm attached to the long arms of wheat chromosomes 6B and 4B, respectively. The breakpoint in T6BS·6BL-6RL is located at a fraction length (FL) of 0.11 in the long arm of T6BS 6BL-6RL and at FL 0.46 in the long arm of T4BS·4BL-6RL. Ti4AS·4AL-6RL-4AL is an intercalary translocation with the breakpoint located at FL 0.06 in the long arm of wheat chromosome 4A. The inserted 6RL segment, with the Hessian fly resistance gene, has a size of 0.7 μm, and is the smallest and, so far, the first radiation-induced intercalary translocation identified in wheat.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-2242
    Keywords: Hessian fly resistance ; Insect antibiosis ; Wheat-rye hybrids ; C-banding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Four wheat-rye lines derived from a cross between hexaploid wheat ‘ND 7532’ and ‘Chaupon’ rye were homogeneous for resistance to biotype L of the Hessian fly,Mayetiola destructor. Because the wheat parent was susceptible and the rye parent was resistant to larval feeding, resistance was derived from rye. Resistance of ‘Chaupon’ and the wheat-rye lines was expressed as larval antibiosis. First-instar larvae died after feeding on plants. Chromosomal analyses using C- and N-banding techniques were performed on plants of each line to identify genomes and structural changes of chromosomes. Results showed that two of the resistant lines were chromosome addition lines carrying either the complete rye chromosome,2R, or only the long arm of2R. The other two resistant lines were identified as being2BS/ 2RL wheat-rye translocation lines. It was concluded, therefore, that the long arm of rye chromosome2R carries a gene or gene complex that conditions antibiosis to Hessian fly larvae and, in the2BS/2RL translocation lines, this rye chromatin is cytologically stable and can be used directly in wheat breeding programs.
    Type of Medium: Electronic Resource
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