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  • 1
    Electronic Resource
    Electronic Resource
    Fluorescence measurements on nanotiter plates (2000)
    [S.l.] : American Institute of Physics (AIP)
    Review of Scientific Instruments 71 (2000), S. 2201-2205 
    Details
    ISSN: 1089-7623
    Source: AIP Digital Archive
    Topics: Physics , Electrical Engineering, Measurement and Control Technology
    Notes: Two different highly sensitive and fast but low-cost instruments for fluorescence measurements on nanotiter plates or other high density sample arrays are presented. Both instruments use 635 nm diode lasers for the detection of Cy5 fluorescence. In the first device all cavities of the nanotiter plate are illuminated simultaneously and the fluorescence is detected spatially resolved by a charge-coupled device camera within a few seconds. The second system uses an on-chip microscanner for the sequential illumination of the samples and the fluorescence is detected by a simple photomultiplier tube. Both instruments have originally been developed for environmental analysis by immunochemical labeling but they can also be used for other medical and biological purposes where analyte concentrations have to be determined. © 2000 American Institute of Physics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1063/1.1150606
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  • 2
    Electronic Resource
    Electronic Resource
    Highly Time Resolved Measurements of OH during POPCORN Using Laser-Induced Fluorescence Spectroscopy (1998)
    Springer
    Journal of atmospheric chemistry 31 (1998), S. 205-225 
    Details
    ISSN: 1573-0662
    Keywords: OH instrument ; laser-induced fluorescence ; OH measurements ; photochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Geosciences
    Notes: Abstract Tropospheric hydroxyl radical (OH) concentrations were measured by laser-induced fluorescence (LIF) during the POPCORN field campaign in August 1994 at a rural site in the North East of Germany. Ambient air spectra were recorded by tuning the laser wavelength over a spectral region covering the Q11(3), Q21(3), and P11(1) rotational transitions of the (0-0) band in the A-X system of OH around 308 nm. The observed spectra clearly identify the OH radical in the atmosphere. Besides the OH absorption lines there was no sign of any other narrow-band spectral structure nearby demonstrating the high specificity of the method. For OH measurements with a typical time resolution of 60–100 seconds per data point the laser wavelength was tuned repetitively over small spectral intervals covering the peak position of the P11(1) OH-line and background positions. A total of 2300 measurements were recorded including diurnal cycles of OH with more than 300 data points. The OH as well as the LIF background signal data will be presented. In a first analysis the background signal will be characterized and the correlation between OH and the ozone photolysis frequency will be derived.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1005868520002
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  • 3
    Electronic Resource
    Electronic Resource
    Intercomparison of Tropospheric OH Measurements by Different Laser Techniques during the POPCORN Campaign 1994 (1998)
    Springer
    Journal of atmospheric chemistry 31 (1998), S. 227-246 
    Details
    ISSN: 1573-0662
    Keywords: OH intercomparison ; laser absorption spectroscopy ; DOAS ; laser induced fluorescence ; LIF ; troposphere
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Geosciences
    Notes: Abstract In-situ OH measurements by laser-induced fluorescence (LIF) spectroscopy and folded long-path differential optical absorption spectroscopy (DOAS) were carried out in a rural environment in North-East Germany as part of the field experiment POPCORN in August 1994. The large set of OH data obtained allowed an intercomparison of both techniques based on relative diurnal profiles and simultaneously measured absolute concentrations. Most of the time the two OH instruments encountered the same air and agreed well in the measured relative diurnal variations. Only on a few occasions the measurements significantly disagreed due to a perturbation of the DOAS measurements by a local OH source in the north-western wind sector. Excluding data from this wind direction, the statistical analysis of 137 data pairs yields a correlation coefficient of r = 0.90 and a weighted linear fit with a slope of 1.09 ± 0.12. The correlations are carefully analyzed. The comparison of both instruments is discussed in the light of newly published effective absorption cross-sections for H2O and O2 that affect the calibration of LIF.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006014707617
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    Paper (German National Licenses)
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