ISSN:
1437-7799
Keywords:
Key words Urinary mononuclear cells
;
IgA nephro-pathy
;
Flow-cytometry
;
cellular immunity
;
Clinico-histopathological correlations
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Background. Urinary mononuclear cells (UMCs) were studied in IgA nephropathy (IgAN) in relation to clinical and histopathological parameters. Methods. A two-color flow-cytometry analysis was used to phenotype and quantitate UMCs in patients with IgAN (n = 37) and those with other kidney diseases (n = 27). Results. The IgAN patients had higher numbers of UMCs than the patients with other kidney diseases. Macrophages (CD14+; P 〈 0.0001) and T lymphocytes (CD3+; P = 0.0001; CD4+, P 〈 0.0001) were predominantly found. In the IgAN patients, the quantities of urinary CD3+, CD14+ cells correlated with impaired renal function; namely, serum creatinine, creatinine clearance, and urinary protein excretion. The UMCs were associated with histopathological changes such as glomerular segmental lesions (r = 0.511; P 〈 0.01 for CD3+; r = 0.623, P 〈 0.0001 for CD4+, and r = 0.552; P 〈 0.001 for CD14+) and the index of glomerular hypercellularity (r = 0.405; P = 0.01 for CD3+ T cells and r = 0.392, P = 0.01 for CD14+ macrophages). Moreover, the UMCs reflected the severity of pathology, as estimated by the glomerular score (r = 0.424; P 〈 0.01 for CD3+; r = 0.458; P 〈 0.01 for CD4+; r = 0.500, P = 0.001 for CD14+ cells). UMCs were weakly correlated with tubulointerstitial changes. Conclusion. UMCs were associated with the clinical stage of IgAN and mirrored the extent of histopathology in the kidney. Flow-cytometry analysis of UMCs may help to predict the course of the disease.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s101570050047
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