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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 67 (1986), S. 0 
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Cells of Chlorella regularis (Artari) Oltmanns (S-50) were grown under solar radiation in Tokyo, using a newly constructed outdoor culture system. The maximum specific growth rate (log2 unit h−1) was about 0.3, which was a little lower than the highest value reported using artificial light in the laboratory. The near ultraviolet light, consisting mostly of UV-A, inhibited the growth from 10 to 40% in summer mornings; the inhibition was not significant in the afternoon. The percentage inhibition was correlated with the total dose of the ultraviolet radiation.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-203X
    Keywords: Abbreviations: DO, dissolved oxygen; 2,4-D, 2,4-dichlorophenoxyacetic acid; MES, 2-(N-morpholino) ethanesulfonic acid; rpm, revolution per minute; NAA, 1-naphthaleneacetic acid; vvm, volume per volume per minute
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract. The effect of the oxygen concentration in the aeration gas on regeneration from rice cells in bioreactor cultures was investigated. The efficiency of regeneration in cultures aerated with over 40% oxygen was higher than that in a flask culture. In the case of a culture in which the dissolved oxygen(DO) was saturated by aeration with air, the efficiency of regeneration was less than the half that of cultures aerated with 40% oxygen. In cultures with the DO levels controlled at 8,10 and 12 mg/l, the efficiency of regeneration was highest at 12 mg/l. In the oxygen-enriched cultures, although cell aggregation was observed and the color of plantlets was relatively pale, more than 90% of them grew into healthy plants.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effect of the oxygen concentration in the aeration gas on regeneration from rice cells in bioreactor cultures was investigated. The efficiency of regeneration in cultures aerated with over 40% oxygen was higher than that in a flask culture. In the case of a culture in which the dissolved oxygen(DO) was saturated by aeration with air, the efficiency of regeneration was less than the half that of cultures aerated with 40% oxygen. In cultures with the DO levels controlled at 8,10 and 12 mg/ℓ, the efficiency of regeneration was highest at 12 mg/ℓ. In the oxygenenriched cultures, although cell aggregation was observed and the color of plantlets was relatively pale, more than 90% of them grew into healthy plants.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Plant cell reports 11 (1992), S. 550-553 
    ISSN: 1432-203X
    Keywords: Dehydration ; Oryza sativa L. ; Plantlet regeneration ; Water content
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary To increase plantlet regeneration frequency, rice callus was dehydrated in a Petri dish with a single layer of filter paper prior to transfer to the regeneration medium. With a 24 h dehydration treatment, the regeneration frequency was increased to 47 %, while the regeneration frequency of the untreated control was less than 5 %. This relatively simple method provides an alternative method for improving the regeneration frequency of rice callus.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1432-072X
    Keywords: UV-A ; Hill reaction ; Photoreactivation ; Anacystis nidulans
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The dose effect curve for the inhibition of p-benzoquinone Hill reaction revealed that the long-wavelength ultraviolet radiation (320–390 nm, UV-A) cannot completely inactivate this reaction in the cyanobacterium, Anacystis nidulans. The inactivated Hill reaction is photoreactivated by visible light. Relative quantum responsivity curve for photoreactivation shows peaks at around 440, 630 nm and a minimum at around 520 nm. The reactivation is completely inhibited by 10 μM of 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU). It is suggested that most part of the photoreactivation is dependent on photosynthetic process. Possible, simultaneous inactivation and reactivation by long-wavelength ultraviolet radiation is suggested as the possible mechanism for inability to cause complete inactivation by the radiation.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Plant cell reports 15 (1996), S. 597-600 
    ISSN: 1432-203X
    Keywords: Albino ; Liquid regeneration medium ; Oryza sativa L. ; Plant regeneration ; Semi-solid regeneration medium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In our study, we investigated the effects of regeneration conditions on both green and albino rice plants (Oryza sativa L.). The regeneration frequency of an albino cell line was compared to a normal cell line obtained from mature seed under two kinds of culture conditions; namely, the static culture on semi-solid regeneration medium and the suspension culture in liquid regeneration medium. The albino cell line, from which only albino plantlets were regenerated, was induced from the albino leaf segments. There were no significant differences in the regeneration frequencies between normal and albino calli on the semisolid regeneration medium. On the other hand, the frequency of regeneration of albino calli was significantly lower than that of the control specifically in the liquid regeneration medium.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Plant cell reports 15 (1996), S. 597-600 
    ISSN: 1432-203X
    Keywords: Key words: Albino, Liquid regeneration medium, Oryza sativa L., Plant regeneration, Semi-solid regeneration medium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract. In our study, we investigated the effects of regeneration conditions on both green and albino rice plants (Oryza sativa L.). The regeneration frequency of an albino cell line was compared to a normal cell line obtained from mature seed under two kinds of culture conditions; namely, the static culture on semi-solid regeneration medium and the suspension culture in liquid regeneration medium. The albino cell line, from which only albino plantlets were regenerated, was induced from the albino leaf segments. There were no significant differences in the regeneration frequencies between normal and albino calli on the semi-solid regeneration medium. On the other hand, the frequency of regeneration of albino calli was significantly lower than that of the control specifically in the liquid regeneration medium.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Plant cell, tissue and organ culture 39 (1994), S. 137-145 
    ISSN: 1573-5044
    Keywords: alginate ; conversion ; encapsulation ; encapsulatable unit ; microcapsule ; scale-up ; self-breaking gel bead ; somatic embryo ; sorter
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Production of encapsulatable unit, scale-up of the production system, and encapsulation technologies were developed as an integrated technology for transplant production using somatic embryos of celery and carrot. Encapsulatable units, somatic embryos improved in their quality for encapsulation, showed 80% conversion without encapsulation and any nutrient supply on a nursery medium in a greenhouse. The high conversion ability was also shown by carrot encapsulatable unit produced with a newly developed 8-liter culture jar, where 5.09×105 torpedo stage embryos were produced and 4.10×104 encapsulatable units were obtained from them. A novel self-breaking gel beads and sustained release microcapsule as an artificial endosperm were developed for the encapsulation of carrot embryos. Also a whole production system for synthetic seed was developed with which 80 thousand beads could be produced per day. The encapsulated carrot embryos showed 52% conversion frequency after sowing on a humid soil in greenhouse.
    Type of Medium: Electronic Resource
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