ISSN:
1432-203X
Keywords:
Abbreviations: DO, dissolved oxygen; 2,4-D, 2,4-dichlorophenoxyacetic acid; MES, 2-(N-morpholino) ethanesulfonic acid; rpm, revolution per minute; NAA, 1-naphthaleneacetic acid; vvm, volume per volume per minute
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract. The effect of the oxygen concentration in the aeration gas on regeneration from rice cells in bioreactor cultures was investigated. The efficiency of regeneration in cultures aerated with over 40% oxygen was higher than that in a flask culture. In the case of a culture in which the dissolved oxygen(DO) was saturated by aeration with air, the efficiency of regeneration was less than the half that of cultures aerated with 40% oxygen. In cultures with the DO levels controlled at 8,10 and 12 mg/l, the efficiency of regeneration was highest at 12 mg/l. In the oxygen-enriched cultures, although cell aggregation was observed and the color of plantlets was relatively pale, more than 90% of them grew into healthy plants.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002990050109
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