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  • 1
    Electronic Resource
    Electronic Resource
    Photosynthetic activity of plant cells solubilized in water-in-oil microemulsions (1991)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 37 (1991), S. 918-921 
    Details
    ISSN: 0006-3592
    Keywords: plant cells ; photosynthesis ; microemulsion ; organic solvents ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: With the aim of possibly extending plant microbiology and photosynthesis beyond the usual applicability in aqueous solution, we investigated the solubilization of plant cells inorganic media with the help of water-in-oil microemulsions. Cells isolated from leaves of Rumex obtusifolius were solubilized in a water/2-ethyl-hexyl-sodiumsulfosuccinate/isooctane system, containing 20% water (v:v) and 240 mM surfactant, and the oxygen evolution/consumption was measured polarographically. Although no oxygen evolution was detectable in the organic medium, the cells were able to carry out photosynthetic oxygen consumption at the expense of ascorbate. To a lesser extent, photosynthetic oxygen consumption was measured using N, N, N′, N′-tetramethyl-p-phenylenediamine as electron donor. The rate of ascorbate photooxidation was linearly related to the concentration of cells.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260371004
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  • 2
    Electronic Resource
    Electronic Resource
    Surfactant-Induced hydrogen production in cyanobacteria (1993)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 42 (1993), S. 1014-1018 
    Details
    ISSN: 0006-3592
    Keywords: cyanobacteria ; surfactant ; hydrogen ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Addition of Tween 85 to aqueous suspensions of Anabaena variabilis induced photosynthetic evolution of hydrogen over a time span of several weeks: As much as 148 nmol H2/h · mg dry weight was produced in the first week by a suspension containing 4.2 mg dry weight of cells and 77 mM Tween 85. The chemical structure of Tween 85 was a necessary prerequisite for inducing hydrogen production, as compounds such as Tween 20, 60, and 80 had a quite different effect. There was a coupling between photosynthetic oxygen evolution and hydrogen evolution: Hydrogen evolution started to be effective only when oxygen evolution subdued. The presence of heterocysts in A. variabilis was also required for the Tween-induced hydrogen production. Based on these observations, possible mechanisms for the photosynthetic effect of Tween 85 are advanced and discussed. © 1993 John Wiley & Sons, Inc.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260420812
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Solubilization and growth of Candida pseudotropicalis in water-in-oil microemulsions (1992)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 40 (1992), S. 167-172 
    Details
    ISSN: 0006-3592
    Keywords: Yeast ; growth ; organic solvent ; microemulsion ; cells ; microbiology ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Some new aspects of microbiology in water-in-oil microemulsions are investigated using Candida pseudotropicalis in a hexadecane solution containing Tween85/Span80 (each 5% wt:wt) as surfactant, and limited amount of water (up to 3%, vol:vol), Microemulsion solutions containing cells up to 10 mg fresh weight per milliliter can be prepared, which display a greater time stability and a much smaller light scattering than aqueous suspensions having the same cell concentration. This is ascribed to a lower aggregation tendency of the cells in the microemulsion environment. It is also shown that C. pseudotropicalis cells are able to grow (up to a factor of approximately 6-7 within a few days) in the microemulsion system containing nutrient medium in the aqueous microphase; but they are also able to grow at the expense of the hexadecane. This is proved with radioactive-labeled hexadecane by measuring the increase of radioactivity in the cells as well as the emission of 14CO2. The growth rate of the cells is then compared with the growth rate of cellular proteins during cell reproduction in the microemulsion system. Two regimes are observed: a first one, in which cells growth rate and protein growth rate proceed parallel to each other; and a second one (established after 0.5-1 day) characterized by depletion of proteins in the microemulsion system. The implications of these findings for cell metabolism in microemulsion and for possible biotechnological applications are discussed.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260400123
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  • 4
    Electronic Resource
    Electronic Resource
    Photosynthetic activity of cyanobacteria in water-in-oil microemulsions (1992)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 40 (1992), S. 173-178 
    Details
    ISSN: 0006-3592
    Keywords: cyanobacteria ; photosynthesis ; organic solvent ; microemulsion ; cells ; microbiology ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The solubilization and the photosynthetic activity of cyanobacteria (Anabaena variabilis) in water-in-oil microemulsions consisting of (Tween85/Span80)/hexadecane/water is investigated. Transparent and stable solutions containing up to 108 cells/mL could be obtained. The physical state and stability of the cells in the microemulsion, as evidenced from optical and electron microscopy, is dependent upon the physical parameters of the system, and in particular on the hydrophylic-lypophilic balance (HLB) of the surfactant system. Conditions could be found, under which the cells in the microemulsion system display photosynthetic activity This was judged by measuring polarographically the oxygen evolution and by studying the photosynthetic activity in the presence of specific inhinbitors.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260400124
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    Paper (German National Licenses)
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