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  • 1
    Electronic Resource
    Electronic Resource
    Extension by Continuity in Pointfree Topology (2000)
    Springer
    Applied categorical structures 8 (2000), S. 475-486 
    Details
    ISSN: 1572-9095
    Keywords: extension by continuity ; convergent filters in frames ; coreflective categories of frames ; special coreflections of frames
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Abstract Bourbaki's extension theorem (General Topology, Part 1, I, 8.5) is known to be one of the most important results concerning the extension of continuous maps. This paper presents a pointfree generalization of this together with various applications to illustrate its remarkable usefulness.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008673803010
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  • 2
    Electronic Resource
    Electronic Resource
    High-level expression of the angiotensin-converting-enzyme-inhibiting peptide, YG-1, as tandem multimers in Escherichia coli (1998)
    Springer
    Applied microbiology and biotechnology 50 (1998), S. 71-76 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract To produce a large quantity of the angiotensin-converting-enzyme(ACE)-inhibiting peptide YG-1, which consists of ten amino acids derived from yeast glyceraldehyde-3-phosphate dehydrogenase, a high-level expression was explored with tandem multimers of the YG-1 gene in Escherichia coli. The genes encoding YG-1 were tandemly multimerized to 9-mers, 18-mers and 27-mers, in which each of the repeating units in the tandem multimers was connected to the neighboring genes by a DNA linker encoding Pro-Gly-Arg for the cleavage of multimers by clostripain. The multimers were cloned into the expression vector pET-21b, and expressed in E. coli BL21(DE3) with isopropyl β-d-thiogalactopyranoside induction. The expressed multimeric peptides encoded by the 9-mer, 18-mer and 27-mer accumulated intracellularly as inclusion bodies and comprised about 67%, 25% and 15% of the total proteins in E. coli respectively. The multimeric peptides expressed as inclusion bodies were cleaved with clostripain, and active monomers were purified to homogeneity by reversed-phase high-performance liquid chromatography. In total, 105 mg pure recombinant YG-1 was obtained from 1 l E. coli culture harboring pETYG9, which contained the 9-mer of the YG-1 gene. The recombinant YG-1 was identical to the natural YG-1 in molecular mass, amino acid sequence and ACE-inhibiting activity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530051258
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  • 3
    Electronic Resource
    Electronic Resource
    Growth ofSchizosaccharomyces sp. HL on supernatant from anaerobically fermented pig waste: Effects of VFA concentration and pH (1990)
    Springer
    Biotechnology letters 12 (1990), S. 309-314 
    Details
    ISSN: 1573-6776
    Source: Springer Online Journal Archives 1860-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Schizosaccharomyces sp. HL utilized almost all of the volatile fatty acids (VFA) up to concentrations of 9.3 g/l in the surface culture using supernatant from anaerobically fermented pig waste as medium. The maximum yield of dry matter (DM) was 5.8 g/l in the optimum conditions of 9.3 g/l VFA, pH 5.1 and 29°C. Lowering of pH was required to obtain the maximum yield of yeast DM, and the optimum pH value rose as the VFA concentration increased.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01093527
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  • 4
    Electronic Resource
    Electronic Resource
    Whole-field strain measurement in a pin-loaded plate by electronic speckle pattern interferometry and the finite element method (1998)
    Springer
    Experimental mechanics 38 (1998), S. 55-60 
    Details
    ISSN: 1741-2765
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract The strain field in an epoxy plate loaded in tension through a steel pin is determined using electronic speckle pattern interferometry (ESPI) and the finite element method (FEM). In a dual-beam illumination speckle interferometer, the in-plane component of the displacement at the plate's surface is accurately measured using a four-step phase-shifting algorithm. Digital image processing algorithms have been developed for noise reduction and strain calculation directly in the computer from the phase map with a strain gage length of about 0.4 mm. A whole-field strain map is obtained, as well as distributions of strain concentration factor, in critical regions near the hole of the plate. FEM is used to perform a nonlinear contact analysis accounting for friction effects at the pin/hole interface. The agreement between experimental results and numerical predictions is good. In terms of speed, accuracy and ease of use, dual-beam ESPI appears to be a superior method of whole-field strain analysis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02321268
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  • 5
    Electronic Resource
    Electronic Resource
    Changes in the Non-Photochemical Quenching of Chlorophyll Fluorescence During Aging of Wheat Flag Leaves (1999)
    Springer
    Photosynthetica 36 (1999), S. 621-625 
    Details
    ISSN: 1573-9058
    Keywords: diurnal variation ; photoinhibition ; photosystem 2 reaction centres ; state transition ; trans-thylakoid membrane proton gradient ; Triticum aestivum ; xanthophyll cycle
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Non-photochemical quenching of chlorophyll fluorescence (qN) and its three components (qNf, qNm, and qNs) in the flag leaves of wheat grown in the field were studied by a fluorometer PAM-2000 on clear days. The diurnal variation patterns of qN in just fully extended (JFEL) and aging leaves (AL) were similar, but qNm declined markedly in JFEL while it remained at a relatively high level in AL under strong sunlight at noon. Furthermore, at midday qNf was higher than qNs in JFEL, but much lower in AL. The results show the relative contributions of different mechanisms in preventing the photosynthetic apparatus from photodamage change during leaf development.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1007012709125
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  • 6
    Electronic Resource
    Electronic Resource
    Correction for Non-Ideal Tracer Pharmacokinetic Disposition by Disposition Decomposition Analysis (DDA) (1998)
    Springer
    Pharmaceutical research 15 (1998), S. 1469-1473 
    Details
    ISSN: 1573-904X
    Keywords: drug tracer ; labeling ; pharmacokinetics ; erythropoietin ; iodination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Purpose. Pharmacokinetic (PK) studies assume that the tracer's PK is equivalent to the parent compound. This assumption is often violated. The aim of this work is to present a method enabling the ideal tracer PK, i.e. the PK of the parent compound, to be predicted from the non-ideal tracer. Methods. The procedure uses a disposition decomposition-recomposition (DDR) that assumes that the labeling mainly changes the elimination kinetics while the distribution kinetics is not significantly affected. In the DDR procedure an elimination rate constant correction factor (kCOR) is determined from a simultaneously fitting to plasma concentration data resulting from an i.v. injection of both the tracer and the parent compound. The correction factor is subsequently used to predict the ideal tracer PK behavior from the disposition function (i.v. bolus response) of the non ideal tracer. Results. The DDR method when applied to plasma level data of erythropoietin (r-HuEPO) and its iodinated tracer (l25I-r-HuEPO) from a high (4000U/kg) and a low (400U/kg) dosing of r-HuEPO in newborn lambs (n = 13) resulted in excellent agreements in the elimination rate corrected dispositions in all cases (r = 0.995, SD = 0.0095). The correction factor did not show a dose dependence (p 〉 0.05). The correction factors were all larger than 1 (kCOR = 1.94, SD = 0.519) consistent with a reduction in the EPO elimination by the iodination labeling. Conclusions. The DDR tracer correction methodology produces a better differentiation of the PK of endogenously produced compounds by correcting for the non-ideal PK behavior of chemically produced tracers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1011922209757
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    Paper (German National Licenses)
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