ISSN:
1573-6776
Source:
Springer Online Journal Archives 1860-2000
Topics:
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Summary A semi-continuous process for the production of human interferon-αc (IFN) from E. coli is described. Harvesting of bacteria, removal of cell debris and concentration were performed by tangentialflow microfiltration (cross-flow microfiltration). Purification was done in a single step by a monoclonal-Ab column. In the process, purified material, 3–9×108 units/mg protein was obtained with a 65% overall yield.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00133826
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