Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    Detection of cancer cells in peripheral blood of stomach cancer patients using RT–PCR amplification of tumour-specific mRNAs (2002)
    Oxford UK : Blackwell Science Ltd.
    Alimentary pharmacology & therapeutics 16 (2002), S. 0 
    Details
    ISSN: 1365-2036
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: RT–PCR amplification of tumour-specific mRNA has been used for the detection of cancer cells in peripheral blood.〈section xml:id="abs1-2"〉〈title type="main"〉Aim:To evaluate the characteristics of the tumour specific mRNA species in peripheral blood of stomach cancer patients.〈section xml:id="abs1-3"〉〈title type="main"〉Methods:We analysed CEA, GalNAc-T, MUC-1, c-MET and hTERT mRNA expression in the stomach cancer cell lines and tissues, lymph nodes and peripheral blood of stomach cancer patients using RT–PCR.〈section xml:id="abs1-4"〉〈title type="main"〉Results:In RT–PCR analysis of the peripheral blood, 4%, 8%, 21%, 46%, and 100% of stomach cancer patients were positive for CEA, GalNAc-T, c-MET, hTERT and MUC-1 mRNA, respectively, but MUC-1 mRNA was also positive in all normal blood samples. The detection of hTERT mRNA was correlated with poor differentiation (P=0.01) and lymph node metastasis (P=0.009). The presence of c-MET mRNA was correlated with T stage (P=0.025), lymph node metastasis (P=0.036), distant metastasis (P=0.031), and stage of the stomach cancer (P=0.023).〈section xml:id="abs1-5"〉〈title type="main"〉Conclusions:Our study suggest that hTERT mRNA in peripheral blood can be a molecular marker for gastric cancer. We also showed that each molecular marker can be correlated with the clinicopathological features of the patients.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2036.16.s2.33.x
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Molecular characterization of cDNAs for two anionic peroxidases from suspension cultures of sweet potato (1999)
    Springer
    Molecular genetics and genomics 261 (1999), S. 941-947 
    Details
    ISSN: 1617-4623
    Keywords: Key words Peroxidase cDNAs ; Suspension cultures ; Environmental stress ; Sweet potato (Ipomoea batatas)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Two cDNAs for anionic peroxidase (PODs), swpa2 and swpa3, were isolated from suspension cultures of sweet potato (Ipomoea batatas), and their expression was investigated with a view to understanding the physiological function of PODs in relation to environmental stresses. Swpa2 (whose putative mature protein product would have a pI value of 4.1) and swpa3 (4.3) encode polypeptides of 358 and 349 amino acids, respectively. The genes from which they were derived are predominantly expressed in cultured cells of sweet potato; transcripts of swpa2 were not detected in any tissues of the intact plant, and transcripts of swpa3 were detected at a low level only in the stem tissue. During cell culture, the expression patterns of the two genes differed; the level of swpa2 RNA progressively increased during cell growth, whereas that of swpa3 reached a maximum at the stationary phase and decreased on further culture. The two genes responded differently to stresses such as wounding or chilling of leaves. Swpa2 was strongly induced 48 h after wounding, but swpa3 was not affected by this treatment. The two genes were also highly expressed upon chilling (4° C), but expression was reduced by prior acclimation at 15° C. In addition, both genes were strongly induced immediately after treatment with ozone, and expression had decreased to the basal level 12 h after treatment. The response of these two genes to stresses such as aging, wounding, and chilling are different from those of the POD genes (swpa1 encoding an anionic product and swpn1 a neutral peroxidase) that we described previously. The responses of the two genes were also different from each other. These results suggest that the two new POD genes are involved in overcoming oxidative environmental stress, and each POD gene may be regulated by cell growth and environmental stress in different ways.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004380051041
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Structure and properties of dynamically cured EPDM and ionomer blends (1991)
    New York, NY : Wiley-Blackwell
    Polymers for Advanced Technologies 2 (1991), S. 31-40 
    Details
    ISSN: 1042-7147
    Keywords: Dynamic curing ; EPDM and ionomer blends ; rheology ; crystallization and Morphology ; Thermoplastic interpenetrating polymer network ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: The structure and properties of dynamically cured ethylene-propylene-diene terpolymer (EPDM) and ionomer blends have been studied. The blends were prepared in a laboratory internal mixer, where EPDM was cured under shear in the presence of ionomer with dicumyl peroxide (DCP) under different shear conditions. The effects of EPDM/ionomer compositions, DCP concentration and the intensity of shear mixing were investigated using capillary rheometer, differential scanning calorimeter (DSC) and scanning electron microscopy (SEM) techniques. Two kinds of poly(ethylene-co-methacrylic acid) ionomers containing different metal ions(Na+ and Zn++) were compared and the effect of the metal ion type for neutralization was considered. The Zn-neutralized ionomer showed better miscibility with EPDM than the Na-neutralized ionomer. It is concluded from the rheological properties, crystallization behavior and morphology that the dynamically cured EPDM and Zn-ion ionomer blends show the behavior of a thermoplastic interpenetrating polymer network (IPN).
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pat.1991.220020105
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...