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  • 1
    Electronic Resource
    Electronic Resource
    Successful expression in pollen of various plant species of in vitro synthesized mRNA introduced by particle bombardment (1995)
    Springer
    Plant molecular biology 28 (1995), S. 337-341 
    Details
    ISSN: 1573-5028
    Keywords: Lilium longiflorum ; Freesia refracta ; Tulipa gesneriana ; GUS mRNA ; particle bombardment ; pollen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Gold particles coated with β-glucuronidase (GUS) mRNA with a 5′ cap structure that had been synthesized in vitro were introduced, by use of a pneumatic particle gun, into pollen grains of lily (Lilium longiflorum), freesia (Freesia refracta) and tulip (Tulipa gesneriana). A fluorometric assay for the GUS activity indicated that in vitro synthesized GUS mRNA introduced into these pollen cells by particle bombardment was successfully expressed. GUS activity in extracts of the bombarded lily pollen became detectable fluorometrically within 30 min after bombardment, peaked at 6 h, then gradually decreased. This activity changed as a function of the developmental stage of the pollen cell of lily.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00020252
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  • 2
    Electronic Resource
    Electronic Resource
    Stable transformation of cultured cells of the liverwortMarchantia polymorpha by particle bombardment (1996)
    Springer
    Transgenic research 5 (1996), S. 337-341 
    Details
    ISSN: 1573-9368
    Keywords: cultured liverwort cells ; hygromycin phosphotransferase (HPT) ; Marchantia polymorpha ; particle bombardment ; stable transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Suspension-cultured cells (A-18 line) of the liverwortMarchanta polymorpha were bombarded by a pneumatic particle gun with plasmid pCH harbouring the hygromycin phosphotransferase (HPT) gene (hpt) under the control of the cauliflower mosaic virus (CaMV) 35 S promoter and the nopaline synthase polyadenylation region. Nine weeks after bombardments, 128 hygromycin-resistant calluses were obtained from an approximate total of 7×106 cells. Ten cell lines chosen randomly were analysed further. Southern blot analysis showed that all of the ten lines contain thehpt gene in the genome, demonstrating that these lines are transformants. An HPT enzyme activity assay confirmed the expression of the gene in all of the transformant lines.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01968943
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  • 3
    Electronic Resource
    Electronic Resource
    Transgenic roots produced by introducing Ri-rol genes into cucumber cotyledons by particle bombardment (1993)
    Springer
    Transgenic research 2 (1993), S. 147-152 
    Details
    ISSN: 1573-9368
    Keywords: rol genes ; transgenic root ; Cucumis sativus ; particle bombardment ; hairy root
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Transformed roots ofCucumis sativus were obtained from cotyledon tissues that had been bombarded with gold particles coated with plasmid pE7.4 using a pneumatic particle gun. This plasmid containsrolA, rolB, rolC genes and ORF 13 of the 7.4 kbEco RI fragment of T-DNA of pRi 1724 isolated fromAgrobacterium rhizogenes MAF 03-01724. The nature of the tissue and the composition of the culture media used greatly influenced the recovery of transformed roots. The transgenic nature of the derived roots was confirmed by the vigorous. highly-branched growth seen on a phytohormone-free medium. The stable integration ofrol genes into the cucumber genome was confirmed by Southern blot analysis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01972608
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  • 4
    Electronic Resource
    Electronic Resource
    Transgenic haploid plants ofNicotiana rustica produced by bombardment-mediated transformation of pollen (1995)
    Springer
    Transgenic research 4 (1995), S. 341-348 
    Details
    ISSN: 1573-9368
    Keywords: transgenic haploid plants ; pollen culture ; particle bombardment ; β-glucuronidase ; neomycin phosphotransferase II
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Immature pollen fromNicotiana rustica was bombarded with gold particles coated with plasmid DNA encoding neomycin phosphotransferase II (NPTII) and β-glucuronidase (GUS) genes which, respectively, are under the control of the cauliflower mosaic virus (CaMV) 35S promoter and nopaline synthase (NOS) terminator in the plasmid. Kanamycin-resistant pollen embryoids were selected from the bombarded pollen cells and two independent lines of transgenic plants were regenerated. Enzyme assays showed that one has both NPTII and GUS activities and the other only weak NPTII activity. Southern blot analyses indicated that the former has a DNA fragment corresponding to the intact expression cassettes for both genes in its genome; whereas the latter lacks intact expression cassettes for both genes and has only the intactnptII coding sequence in its genome. The transgenic plants of both lines have 24 chromosomes, confirming haploidy, and they are infertile. These results indicate that transgenic haploid plants can be produced directly by the bombardment-mediated transformation of immature pollen.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01972531
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  • 5
    Electronic Resource
    Electronic Resource
    In situ hybridization to metaphase chromosomes in six species ofPhaseolus andVigna using ribosomal DNA as the probe (1994)
    Springer
    Journal of plant research 107 (1994), S. 365-369 
    Details
    ISSN: 1618-0860
    Keywords: In situ hybridization ; Phaseolus ; Ribosomal DNA ; Somatic metaphase chromosome ; Vigna
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In situ hybridization with a biotin-labeled rice ribosomal DNA (rDNA) probe to the somatic metaphase chromosomes of six species ofPhaseolus andVigna (P. angularis, P. calcaratus, P. coccineus, P. vulgaris, V. sesquipedalis andV. sinensis) was done to determine the sites of rDNA. Hybridization signals were present in the terminal and subterminal chromosome regions of each of the six species. The number of rDNA sites was two inP. angularis andP. calcaratus, four inP. coccineus andP. vulgaris, and six inV. sesquipedalis andV. sinensis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02344058
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  • 6
    Electronic Resource
    Electronic Resource
    Transient expression of β-glucuronidase in plastids of various plant cells and tissues delivered by a pneumatic particle gun (1995)
    Springer
    Journal of plant research 108 (1995), S. 235-240 
    Details
    ISSN: 1618-0860
    Keywords: α-amanitin ; β-glucuronidase ; Particle gun ; Plastid transformation ; Transient expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Chloroplast expression plasmids pTRBCL-GUS (tobaccorbcL promoter-gusA-tobaccorbcL terminator) and pHHU3004 (spinach ‘x gene’ promoter-gusA-spinachrbcL terminator) and a control nuclear expression plasmid pBI221 (CaMV 35S promoter-gusA-NOS terminator) were introduced separately into cultured cells and tissues of tobacco andArabidopsis thaliana, as well as into cultured cells of the lower land plants liverwort and hornwort by a pneumatic particle gun. The pTRBCL-GUS and pHHU3004 plasmids produced many blue spots in the BY-2 cells and the roots ofArabidopsis thaliana, but not in any of the green cells or tissues. The results suggest that the pTRBCL-GUS and pHHU3004 plasmids are expressed more in proplastids and amyloplasts than in chloroplasts. GUS activities of the BY-2 cells bombarded with pTRBCL-GUS and pHHU3004 were insensitive to α-amanitin treatment (10 and 50 μg/ml), while that of the cells with pBI221 greatly decreased by the same treatment. Hence, it is likely that the pTRBCL-GUS and pHHU3004 plasmids were substantially expressed in the proplastids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02344348
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  • 7
    Electronic Resource
    Electronic Resource
    Bombardment-mediated transformation of plant cells (1994)
    Springer
    Journal of plant research 107 (1994), S. 117-123 
    Details
    ISSN: 1618-0860
    Keywords: Integration ; Organelle ; Particle gun ; Pollen ; Transformation ; Transgenic plants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Significance of bombardment-mediated transformation in plant research is discussed. Apart from general subjects related to this transformation study, a particular emphasis has been placed on the pollen and organelle transformation and on integration mechanism. Over 70 literatures related to this field are cited here.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02346006
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  • 8
    Electronic Resource
    Electronic Resource
    Stable transformation ofArabidopsis with thebar gene using particle bombardment (1994)
    Springer
    Transgenic research 3 (1994), S. 279-286 
    Details
    ISSN: 1573-9368
    Keywords: Arabidopsis thaliana ; transgenic plants ; particle bombardment ; bar gene ; phosphinothricin acetyltransferase ; late-flowering mutant
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A plasmid pARK 22 harbouring thebar gene encoding phosphinothricin acetyltransferase (PAT) under the control of the cauliflower mosaic virus (CaMV) 35S promoter and nopaline synthase (NOS) terminator was constructed and introduced into root sections ofArabidopsis thaliana using the pneumatic particle gun. The root sections that had been bombarded with this plasmid gave four to eight times higher yield of drug-resistant calluses than those sections bombarded with pCaMVNEO or pCH, which respectively contain the neomycin phosphotransferase and hygromycin phosphotransferase genes. Among a number of primary transformant (T0) plants obtained from independent bialaphos-resistant calluses, three were studied by Southern blot hybridization and PAT enzyme activity analyses, confirming the stable integration of the foreign gene into theArabidopsis genome and its expression in plants. The progeny analysis showed transmission of the foreign gene and its expression in up to the T2 generation. Some of the T1 progeny showed morphological abnormalities. Thus, thebar gene can be used effectively to allow selection of transgenicA. thalianna plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01973587
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