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  • 1
    ISSN: 1365-3083
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Medizin
    Notizen: Contradictory reports regarding the ability of complement receptor type 2 (CR2,CD21) on normal B cells to activate complement (C′) via the alternative pathway (AP), prompted us to compare the performance of human peripheral blood B cells and the Epstein–Barr virus-positive Burkitt's lymphoma cell line, Raji (a well characterized AP activator) by using flow cytometry. Measured in terms of the membrane deposition of C3 fragments per cell, Raji cells were significantly (6- to 26-fold) more effective as complement activators than were normal B cells. Raji cells were also found to express approximately four to five times as many CR2 as normal B cells. In addition, they distinguished themselves by displaying a greater Ca2+-dependent activation, with pooled normal human sera (NHS) as the complement source, and by degrading unprotected C3b fragments from iC3b to C3dg/C3d at a significantly lower rate than the B cells. The Ca2+ dependency of Raji cell activation was found to be partially a result of classical pathway (CP) triggering by specific antibodies in the NHS, although other triggering mechanisms may also be involved. If the influence of these variations between Raji cells and normal B cells was excluded, by relating deposition of anti-C3d-reactive fragments, during AP activation, to the number of CR2 expressed, the difference in performance between the two cell types was found to be insignificant.
    Materialart: Digitale Medien
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  • 2
    ISSN: 1439-0523
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: The study examined the effect of ploidy elevation through unreduced gametes on transgene expression in potato. Tetraploid transgenic progenies were obtained from one tetraploid potato cultivar crossed with 2n pollen producing diploid clones harbouring an exogenous transgene (cry3Aa). Both single- and multiple-insert diploid transgenic lines that were regenerated by Agrobacterium tumefaciens leaf disc inoculation were used in crosses. A DAS-ELISA system and no-choice feeding bioassay enabled characterization of the parental lines as either ‘high’ or low’ expressers of the Cry3Aa protein. High Cry3Aa expression was observed for both single-insert transgenic diploids and their 4x-2x progeny. On the contrary, 68% of 4x-2x progeny derived from a multiple-insert, diploid transgenic had significantly reduced Cry3Aa expression compared with the parent, with 32% demonstrating nearly complete silencing of the transgene. Multiple copies of a transgene, like homologous native genes, may be susceptible to transgene silencing following polyploidization. Therefore, incorporation of exogenous transgenes into a true potato seed (TPS) production system is feasible if a single-insert diploid parent is used. Gene-centromere mapping of the cry3Aa transgene demonstrated that a non-transgenic refuge might be naturally created in a TPS hybrid system through genetic recombination.
    Materialart: Digitale Medien
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  • 3
    Digitale Medien
    Digitale Medien
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 29 (1964), S. 0 
    ISSN: 1750-3841
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: The influence of many individual factors on the uptake of sulfur dioxide by apricot, peach, and pear tissue before drying was tested in equipment designed to permit accurate control over sulfuring conditions. Exploratory trials showed that estimates of sulfur dioxide uptake were unreliable unless standardized sampling methods were used. Absorption rate was affected appreciably by type of fruit, surface area as influenced by size, and the use of irrigation, and less so by variety and maturity. Sulfur dioxide uptake through the skin was slow. Combinations of gas concentration and exposure time were the most important processing variables affecting absorption. Variation in air speed and temperature had little influence upon absorption. Holding cut fruits for up to 10 hr before sulfuring did not cause a decrease in gas uptake. Excess water on the fruit surface from spraying tended to decrease absorption, while steam-blanching retarded it appreciably. Any disorganization of tissue, whether from increasing maturation, storage, or processing, retarded sulfur dioxide uptake, probably through sealing, of penetration pathways. The ready desorption of sulfur dioxide from tissue removed from the sulfuring environment indicated low chemical fixation at that stage.
    Materialart: Digitale Medien
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  • 4
    Digitale Medien
    Digitale Medien
    [s.l.] : Nature Publishing Group
    Nature 184 (1959), S. 356-357 
    ISSN: 1476-4687
    Quelle: Nature Archives 1869 - 2009
    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
    Notizen: [Auszug] The starting material was a 0 0005 in. thick cross-rolled molybdenum foil of 99-90 per cent purity supplied by Messrs. Metro-Cutanit. Further thinning was achieved by electrolytic polishing in a solution of 25 per cent sulphuric acid in methanol using a technique similar to that described by ...
    Materialart: Digitale Medien
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  • 5
    Digitale Medien
    Digitale Medien
    [s.l.] : Nature Publishing Group
    Nature 162 (1948), S. 225-225 
    ISSN: 1476-4687
    Quelle: Nature Archives 1869 - 2009
    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
    Notizen: [Auszug] IN a communication under the above title, published in Nature of May 31, 1947, p. 746, J. L. Mongar and A. Wassermann report some experiments on the de-swelling of calcium alginate fibres in salt solutions. They noted that water-swollen alginate fibres when placed in various alkali-salt ...
    Materialart: Digitale Medien
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  • 6
    Digitale Medien
    Digitale Medien
    [s.l.] : Nature Publishing Group
    Nature 217 (1968), S. 442-444 
    ISSN: 1476-4687
    Quelle: Nature Archives 1869 - 2009
    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
    Notizen: [Auszug] This new method, which uses a solid state reaction to create the fibres, has been demonstrated using alloys in the gold?nickel system. The phase diagram for this system, which contains a simple miscibility gap, is shown in Fig. 1. In a series of experiments on the equiatomic alloy, the specimens ...
    Materialart: Digitale Medien
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  • 7
    Digitale Medien
    Digitale Medien
    [s.l.] : Nature Publishing Group
    Nature 201 (1964), S. 1020-1021 
    ISSN: 1476-4687
    Quelle: Nature Archives 1869 - 2009
    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
    Notizen: [Auszug] The alloy was prepared by melting together appropriate amounts of high-purity gold and nickel in a recrystallized alumina crucible under a vacuum of 10-5 mm mercury. It was then remelted several times to ensure its homogeneity, encapsulated in an evacuated quartz tube, solution treated for 1 h at ...
    Materialart: Digitale Medien
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  • 8
    Digitale Medien
    Digitale Medien
    [s.l.] : Nature Publishing Group
    Nature 195 (1962), S. 169-169 
    ISSN: 1476-4687
    Quelle: Nature Archives 1869 - 2009
    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
    Notizen: [Auszug] Recrystallized niobium and molybdenum wires were irradiated for periods of 3, 6, 9 and 12 months at 40 C. in the reactor BEPO at the Atomic Energy Research Establishment, Harwell. The irradiations were carried out in a vertical hole in the reactor where the epithermal neutron flux was about ...
    Materialart: Digitale Medien
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  • 9
    Digitale Medien
    Digitale Medien
    [s.l.] : Nature Publishing Group
    Nature 195 (1962), S. 374-375 
    ISSN: 1476-4687
    Quelle: Nature Archives 1869 - 2009
    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
    Notizen: [Auszug] Fig. 1. Effect of strain-rate, e, on the room temperature yield stresses of (a) niobium, and (b) tantalum Fig. 1 shows the yield stresses of the two materials at room temperature plotted as functions of Iog10e, where e is the strain-rate in sec.-1. In the case of niobium three linear ranges are ...
    Materialart: Digitale Medien
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  • 10
    Digitale Medien
    Digitale Medien
    [s.l.] : Nature Publishing Group
    Nature 183 (1959), S. 1542-1543 
    ISSN: 1476-4687
    Quelle: Nature Archives 1869 - 2009
    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
    Notizen: [Auszug] Part of a typical fracture edge is shown in Fig. 1. It is made up mainly of straight-line segments which often occupy one complete grain (see A in Fig. 1). Within about 0 -5 mm. of each fracture edge a number of subsidiary cracks are observed (see B in Fig. 1) and often these are arranged in a ...
    Materialart: Digitale Medien
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