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  • 1
    Digitale Medien
    Digitale Medien
    Altered levels and distribution of microtubule-associated proteins before disease onset in a mouse model of amyotrophic lateral sclerosis (2003)
    Oxford, UK : Blackwell Science Ltd
    Journal of neurochemistry 84 (2003), S. 0 
    Details
    ISSN: 1471-4159
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Medizin
    Notizen: Alterations of the axonal transport and microtubule network are potential causes of motor neurodegeneration in mice expressing a mutant form of the superoxide dismutase 1 (SOD1G37R) linked to amyotrophic lateral sclerosis (ALS). In the present study, we investigated the biology of microtubule-associated proteins (MAPs), responsible for the formation and stabilization of microtubules, in SOD1G37R mice. Our results show that the protein levels of MAP2, MAP1A, tau 100 kDa and tau 68 kDa species decrease significantly as early as 5 months before onset of symptoms in the spinal cord of SOD1G37R mice, whereas decrease in levels of tau 52–55 kDa species is most often noted with the manifestation of the clinical symptoms. Interestingly, there was no change in the protein levels of MAPs in the brain of SOD1G37R mice, a CNS organ spared by the mutant SOD1 toxicity. Remarkably, as early as 5 months before disease onset, the binding affinities of MAP1A, MAP2 and tau isoforms to the cytoskeleton decreased in spinal cord of SOD1G37R mice. This change correlated with a hyperphosphorylation of the soluble tau 52–55 kDa species at epitopes recognized by the antibodies AT8 and PHF-1. Finally, a shift in the distribution of MAP2 from the cytosol to the membrane is detected in SOD1G37R mice at the same stage. Thus, alterations in the integrity of microtubules are early events of the neurodegenerative processes in SOD1G37R mice.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1046/j.1471-4159.2003.01505.x
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  • 2
    Digitale Medien
    Digitale Medien
    Identification of a novel family of G protein-coupled receptor associated sorting proteins (2004)
    Oxford, UK : Blackwell Science Ltd
    Journal of neurochemistry 89 (2004), S. 0 
    Details
    ISSN: 1471-4159
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Medizin
    Notizen: During the past few years several new interacting partners for G protein-coupled receptors (GPCRs) have been discovered, suggesting that the activity of these receptors is more complex than previously anticipated. Recently, candidate G protein-coupled receptor associated sorting protein (GASP-1) has been identified as a novel interacting partner for the delta opioid receptor and has been proposed to determine the degradative fate of this receptor. We show here that GASP-1 associates in vitro with other opioid receptors and that the interaction domain in these receptors is restricted to a small portion of the carboxyl-terminal tail, corresponding to helix 8 in the three-dimensional structure of rhodopsin. In addition, we show that GASP-1 interacts with COOH-terminus of several other GPCRs from subfamilies A and B and that two conserved residues within the putative helix 8 of these receptors are critical for the interaction with GASP-1. In situ hybridization and northern blot analysis indicate that GASP-1 mRNA is mainly distributed throughout the central nervous system, consistent with a potential interaction with numerous GPCRs in vivo. Finally, we show that GASP-1 is a member of a novel family comprising at least 10 members, whose genes are clustered on chromosome X. Another member of the family, GASP-2, also interacts with the carboxyl-terminal tail of several GPCRs. Therefore, GASP proteins may represent an important protein family regulating GPCR physiology.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1471-4159.2004.02411.x
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  • 3
    Digitale Medien
    Digitale Medien
    Search for Poliovirus in Specimens from Patients with the Post-Polio Syndrome (1995)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 753 (1995), S. 0 
    Details
    ISSN: 1749-6632
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Allgemeine Naturwissenschaft
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1749-6632.1995.tb27549.x
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  • 4
    Digitale Medien
    Digitale Medien
    The coupling between extra- and intracellular electric potentials in Bidens pilosa L. (1993)
    Oxford, UK : Blackwell Publishing Ltd
    Plant, cell & environment 16 (1993), S. 0 
    Details
    ISSN: 1365-3040
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Notizen: The extracellular electrical potential in Bidens pilosa has been compared with the transmembrane potential in hypocotyl cells. We found that variations in the extracellular electrical potential, induced by different KCI concentrations, were identical to the variations of the transmembrane potential. In order to explain the strong correlation between the extracellular potential and the transmembrane potential, an interpretative model based on the electrical diagram of the plant was tested. From our data, we found that: (1) the symplast behaves as a low resistance pathway compared with the apoplast; and (2) the cell wall contributes weakly (20% at the most) to the transmembrane potential. It is concluded that each hypocotyl cell is strongly coupled electrically with its neighbour. This property, together with the high apoplast resistance, accounts for the similarity in the extracellular and transmembrane potential measurements. These properties, which are characteristic of excitable organs, support the existence of action potentials in Bidens.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1365-3040.1993.tb00481.x
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  • 5
    Digitale Medien
    Digitale Medien
    Ectopic Substance P and Calcitonin Gene-related Peptide Immunoreactive Fibres in the Spinal Cord of Transgenic Mice Over-expressing Nerve Growth Factor (1995)
    Oxford, UK : Blackwell Publishing Ltd
    European journal of neuroscience 7 (1995), S. 0 
    Details
    ISSN: 1460-9568
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Medizin
    Notizen: The aim of this study was to investigate the in vivo effects of CNS over-expression of nerve growth factor (NGF) on primary sensory neurons. To achieve this objective a transgenic mouse model was generated which bore a chick NGF gene driven by the myelin basic protein promoter. Northern blot analysis demonstrated that high levels of NGF mRNA were detected in the spinal cord of adult transgenic mice. Using immunocytochemistry NGF-immunoreactive (IR) oligodendrocytes were observed throughout the white matter. Furthermore, numerous ectopic substance P (SP)- and calcitonin gene-related peptide (CGRP)-IR fibres were detected in the white matter of the spinal cord of transgenic mice. NGF-IR oligodendrocytes and ectopic SP- and CGRP- fibres were entirely absent from control mice. In the cervical and lumbar dorsal root ganglia, the percentages of SP-IR neurons were significantly higher in transgenic mice when compared with controls. At the electron microscope level, ectopic SP- and CGRP-IR fibres were characterized as unmyelinated axons and axonal boutons. SP co-localized with CGRP in some of those axonal boutons and fibres. Capsaicin treatment of adult mice completely abolished the ectopic SP-IR fibres, confirming their primary sensory origin. Our results indicate that primary sensory neurons are responsive to NGF over-expression in the CNS. Ectopic SP- and CGRP-IR fibres in the white matter are likely to represent collateral sprouts of the central processes of the dorsal root ganglion cells which were triggered by NGF over-expressed in the myelinating oligodendrocytes in the spinal cord of transgenic mice.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1460-9568.1995.tb00625.x
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  • 6
    Digitale Medien
    Digitale Medien
    Selection of Mixed Cultures for Meat Fermentation (1989)
    Oxford, UK : Blackwell Publishing Ltd
    Journal of food science 54 (1989), S. 0 
    Details
    ISSN: 1750-3841
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung
    Notizen: Strains of Pediococcus cerevisiae, Pediococcus acidilactici, Lactobacillus plantarum, Lactobacillus casei and Micrococcus varians were studied with the aim of preparing mixed cultures of compatible strains for meat fermentations. Addition of 0.18% nitrite was slightly inhibitory while addition of 7% of a spice mix proved stimulatory. Antibiosis was observed between lactobacilli and pediococci. Micrococcus varians did not produce inhibitory substances toward other cultures and was insensitive to bacteriocins produced by the lactobacilli or the pediococci. Antibioses observed using disk assays were confirmed in mixed acidification trials, and mixes of some fast-acidifying strains were unsuccessful.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1111/j.1365-2621.1989.tb07894.x
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  • 7
    Digitale Medien
    Digitale Medien
    Reduced number of unmyelinated sensory axons in peripherin null mice (2002)
    Oxford, UK : Blackwell Science Ltd
    Journal of neurochemistry 81 (2002), S. 0 
    Details
    ISSN: 1471-4159
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Medizin
    Notizen: Peripherin is a type III intermediate filament (IF) abundantly expressed in developing neurons, but in the adult, it is primarily found in neurons extending to the peripheral nervous system. It has been suggested that peripherin may play a role in axonal elongation and/or cytoskeletal stabilization during development and regeneration. To further clarify the function of peripherin, we generated and characterized mice with a targeted disruption of the peripherin gene. The peripherin null mice were viable, reproduced normally and did not exhibit overt phenotypes. Microscopic analysis revealed no gross morphological defects in the ventral and dorsal roots, spinal cord, retina and gut, but protein analyses showed increased levels of the type IV IF α-internexin in ventral roots of peripherin null mice. Whereas the number and caliber of myelinated motor and sensory axons in the L5 roots remained unchanged in peripherin knockout mice, there was a substantial reduction (∼ 34%) in the number of L5 unmyelinated sensory fibers that correlated with a decreased binding of the lectin IB4. These results demonstrate a requirement of peripherin for the proper development of a subset of sensory neurons.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1046/j.1471-4159.2002.00853.x
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  • 8
    Digitale Medien
    Digitale Medien
    Impaired learning and LTP in mice expressing the carboxy terminus of the Alzheimer amyloid precursor protein (1997)
    [s.l.] : Nature Publishing Group
    Nature 387 (1997), S. 500-505 
    Details
    ISSN: 1476-4687
    Quelle: Nature Archives 1869 - 2009
    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
    Notizen: [Auszug] Transgenic mice were produced by using a construct containing an APP complementary DNA fragment encoding amino acids 591 to 695, which spans the amyloid-forming portion and the carboxy terminus of the human amyloid precursor protein, cloned into the first exon of the human neurofilament NF-L gene ...
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1038/387500a0
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  • 9
    Digitale Medien
    Digitale Medien
    The structure of a human neurofilament gene (NF-L): A unique exon-intron organization in the intermediate filament gene family
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/Gene Structure and Expression 909 (1987), S. 10-20 
    Details
    ISSN: 0167-4781
    Schlagwort(e): (Human DNA) ; Cloning ; Cosmid library ; Exon-intron organization ; Neurofilament gene ; Nucleotide sequence
    Quelle: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Thema: Biologie , Chemie und Pharmazie , Medizin , Physik
    Materialart: Digitale Medien
    URL: http://linkinghub.elsevier.com/retrieve/pii/0167-4781(87)90041-8
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  • 10
    Digitale Medien
    Digitale Medien
    Cloning of a cDNA encoding the smallest neurofilament protein from the rat
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/Gene Structure and Expression 825 (1985), S. 398-404 
    Details
    ISSN: 0167-4781
    Schlagwort(e): Cloning ; Gene structure ; Neurofilament protein ; Nucleic acid sequence
    Quelle: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Thema: Biologie , Chemie und Pharmazie , Medizin , Physik
    Materialart: Digitale Medien
    URL: http://linkinghub.elsevier.com/retrieve/pii/0167-4781(85)90067-3
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