Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    In Vitro Stimulation with Glutamic Acid Decarboxylase (GAD65) Leads to an Oligoclonal Response of Peripheral T-Cells in an IDDM Patient (1995)
    Oxford, UK : Blackwell Publishing Ltd
    Scandinavian journal of immunology 42 (1995), S. 0 
    Details
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The enzyme glutamic acid decarboxylase (GAD65) is a major autoantigen in insulin-dependent diabetes mellitus (IDDM). To study T-cell reactivity towards GAD, peripheral blood leucocytes from seven patients with IDDM and five control subjects were stimulated in vitro with recombinant GAD. All diabetics studied were heterozygous for diabetes-associated HLA alleles, i. e. HLA-DRB1*03, *04-DQBl*0302, *0201. A single IDDM subject (no. GAD65.05) revealed a strong response against GAD65. After stimulation, his T-cell receptor β (TCRBV) usage was found to be oligoclonal. The sequence analysis of the putative peptide binding region of the T-cell receptor (CDR3 region) of 37 GAD-reactive T-cell clones revealed no common CDR3 motif. The stimulation of GAD-reactive T-cells could be inhibited with anti-class II monoclonal antibodies, indicating a class II restricted T-cell response. In addition, GAD65-responsive T-cells revealed a Thl cytokine response pattern. The author's data suggest that GAD-reactive T-cells of Thl phenotype can be obtained after in vitro stimulation of peripheral blood leucocytes from an HLA-DRBl*03/*04 heterozygous IDDM patient. The lack of a common CDR3 motif suggests the absence of an immunodominant T-cell epitope in that patient, or may indicate receptor repertoire spreading of peripheral T-lymphocytes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-3083.1995.tb03710.x
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Properdin factor B-polymorphism in the population of Hessen, Germany (1978)
    Springer
    International journal of legal medicine 81 (1978), S. 125-131 
    Details
    ISSN: 1437-1596
    Keywords: Properdin factor B ; polymorphism ; Blutgruppen ; Properdinfaktor B ; Polymorphismus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Law
    Description / Table of Contents: Zusammenfassung Der Polymorphismus des Properdin-Faktor B (Bf, C3-Proaktivator) wurde an einer Bevölkerungsstichprobe aus Hessen mittels Agarosegelelektrophorese und Immunofixation untersucht. Bei 522 Personen wurden sieben verschiedene Phänotypen beobachtet und die folgenden Allelfrequenzen berechnet: BfS=0,7998, BfF=0,1772, BfS0,7=0,0163 und BfF1=0,0077. Untersuchungen an 100 Familien mit 198 Kindern und 30 Mutter-Kind-Kombinationen bestätigen den angenommenen autosomal kodominanten Vererbungsmodus.
    Notes: Summary The polymorphism of properdin factor B (Bf, C3 proactivator) in a population sample from Hessen, Germany has been investigated by agarose gel electrophoresis and immunofixation. In 522 unrelated individuals seven different phenotypes were observed and the following allele frequencies calculated: BfS=0.7998, BfF=0.1772, BfS0.7=0.0163 and BfF1=0.0077. Investigations of 100 families with 198 children and 30 mother-child combinations support the assumed autosomal codominant way of inheritance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00203872
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Das Blutgruppengutachten: Beziehungen zwischen Untersuchungsumfang und Zuverlässigkeit der Vaterschaftsfeststellung (1983)
    Springer
    International journal of legal medicine 90 (1983), S. 35-44 
    Details
    ISSN: 1437-1596
    Keywords: Blood group expertise ; Exclusion chance for non-fathers ; Prior probability ; Blutgruppengutachten ; Vaterschaftsausschlußchance ; A-priori-Wahrscheinlichkeit
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Law
    Description / Table of Contents: Zusammenfassung Im Vaterschaftsgutachten läßt sich derzeit eine allgemeineVaterschaftsausschlußchance (AVACH) von 99,995% unter Verwendung hinreichend gesicherter Blutgruppensysteme erreichen. Bei komplizierter Beweislage muß das Gericht entscheiden, welche biostatistischen Werte im Einzelfall zur Feststellung der Vaterschaft benötigt werden. Für Routinefälle genügt dagegen die im derzeit üblichen Gutachten erreichte AVACH von ca. 95%. Die Aufschlüsselung von 263 Kindschaftssachen ergab eine durchschnittliche realistische A-priori-Wahrscheinlichkeit von 83,3%, bei Mehrmannsachen (n=52) sogar von 90,3%. Letzteres trifft annähernd (89,5%) auch für die 67 Zweimannsachen in Ehelichkeitsanfechtungsklagen zu. Da sich unter „nachgeschobenen” Eventualvätern nur selten der wirkliche Vater findet (kein realistisches A-priori), bleiben 5–15% der Kinder ohne bekannten Vater.
    Notes: Summary In affiliation cases a combined exclusion chance for non-fathers of 99.995% is obtained by the examination of well-established blood group systems. In complicated cases, i.e. if known putative fathers are unavailable, the biostatistical limits for the ascertainment of paternity are obviously very high. They have to be determined by court in each particular case. For routine cases the application of an extended basic blood group expertise, including 19 systems with an combined exclusion chance of 95.17% is considered to be sufficient. The analysis of 263 own filiation cases from 1979 to 1982 yielded an average realistic prior probability of paternity of 83.3%, in 52 many-man affairs even of 90.3%. A similar percentage (89.5%) was observed in 67 two-man affairs of contested legitimacy. Since the father is rarely found among men included at a later stage the rate of children without known father is estimated at 5–15%.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01886065
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Untersuchungen zum Polymorphismus der intraerythrocytären Esterase D (Es D) mittels Hochspannungselektrophorese auf Agarosegel (1974)
    Springer
    International journal of legal medicine 75 (1974), S. 179-182 
    Details
    ISSN: 1437-1596
    Keywords: Blutgruppen ; Esterase D ; Esterase D-Polymorphismus ; Vaterschaftsgutachten
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Law
    Description / Table of Contents: Zusammenfassung Es wird über eine neue, verbesserte Methode zur Darstellung des Polymorphismus der intraerythrocytären Esterase D (Es D) berichtet. Die Hochspannungsdünn-schichtelektrophorese auf Agarosegel erlaubt eine sichere Differenzierung der drei Isoenzymmuster. Die Allelhäufigkeiten bei einer 510 Personen umfassenden hessischen Population betragen: Es D1 = 0,8882, Es D2 = 0,1118; seltene Varianten wurden bisher nicht beobachtet. Familienuntersuchungen bestätigen den angenommenen autosomal kodominanten Vererbungsmodus.
    Notes: Summary An improved method for the demonstration of polymorphism of red cell esterase D (Es D) by means of high voltage agarose gel electrophoresis is described. The three isozyme patterns can by distinguished clearly. The allele frequencies in 510 unrelated individuals from Hessen/Germany are. Es D1 = 0.8882, Es D2 = 0.1118; rare phenotypes were not observed thus far. Family investigations support the supposed way of autosomal codominant inheritance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01882700
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    Prevalence of HLA-DQ beta chain non-asp alleles in type I (insulin-dependent) diabetics with young and older ages of onset (1991)
    Springer
    Journal of molecular medicine 69 (1991), S. 687-689 
    Details
    ISSN: 1432-1440
    Keywords: Adult-onset diabetes ; HLA-DQβ ; Polymerase chain reaction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Particular HLA-DQβ chain alleles were reported as immunogenetic markers of type I diabetes mellitus with young onset of the disease. In a homogenous German population, we studied HLA-DR specificities and HLA-DQβ chain alleles in young-onset (〈21 years of age;n=185) and adult-onset (〉40 years of age;n=48) insulin-dependent diabetics. In both cohorts of type I diabetics, the HLA-DR3 and -DR4 specificities were significantly increased. The presence of an HLA haplotype with an amino acid other than aspartic acid at position 57 of the DQβ chain was significantly associated with type I diabetes in both cohorts (etiologic fraction:93% and 73%). We conclude that the presence of DNA sequences coding for an amino acid other than aspartic acid at the 57th position of the DQβ chain provides a molecular risk marker for type I diabetes of both and adult onset.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01649437
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    HLA-DR3 and HLA-DR5 confer risk for autoantibody positivity against the thyroperoxidase (mic-TPO) antigen in healthy blood donors (1993)
    Springer
    Journal of molecular medicine 71 (1993), S. 221-225 
    Details
    ISSN: 1432-1440
    Keywords: Autoantibodies ; Autoimmune thyroid diseases ; Human leukocyte antigen ; Microsomal thyroid antigen ; Thyroid autoimmunity ; Thyroid peroxidase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The prevalence of circulating autoantibodies against thyroperoxidase (mic-TPO) was determined in 3,000 healthy blood donors (age range: 23 to 60 years) from the Hamburg area. Of the blood donors, 153 (5.1 %) were found to have high titer of mic-TPO (〉 350 IU/ml). Only two auto-antibody positive subjects (0.06%) were chemically hyper- and hypothyroid, respectively. Analysis of HLA-DR specificities revealed that HLA-DR specificities DR3 and DR5 were significantly increased when compared to controls (n=1,863). Comparison of the autoantibody-positive probands with a group of disease controls, i.e., Graves' patients and patients with lymphocytic thyroiditis, revealed a higher prevalence of HLA-DR3-positive HLA haplotypes in the disease controls when compared to autoantibody positives. Individuals with a mic-TPO level greater than 2,000 IU/ml were almost exclusively found to have one HLA-DR3 or HLA-DR5 positive HLA haplotype. We conclude that a high prevalence of hightiter mic-TPO can be found in healthy blood donors. Circulating signs of thyroid autoimmunity were associated with HLA specificities also found to be associated with autoimmune thyroid diseases.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00180105
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 7
    Electronic Resource
    Electronic Resource
    HLA-DRB3 gene alleles in Caucasian patients with Graves' disease (1992)
    Springer
    Journal of molecular medicine 70 (1992), S. 956-960 
    Details
    ISSN: 1432-1440
    Keywords: Graves' disease ; HLA-DRB3 alleles ; Endocrine ophthalmopathy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Graves' disease (GD) is a human leukocyte antigen (HLA) linked organ-specific autoimmune disease. In German GD patients the disease is associated with HLA specificities of the HLA-DRw52 family (HLA-DR3, -DR5, and DR6; HLA-DRB3 positive HLA haplotypes). Recently, a strong association with a HLA-DRB3 restriction fragment length polymorphism gene has been described. To study HLA-DRB3 alleles and their association with the disease, a large cohort of controls (n = 3724) and GD patients (n = 304) was analyzed. HLA-DR allelic combinations revealed an increase in HLA-DR3/DR5 heterozygous patients (relative risk 2.9; P〈0.001). HLA-DRB3 alleles, as defined by DNA typing in HLA-DR matched groups revealed a significant increase in DRB3*0101 homozygosity (relative risk 17.5; P〈 0.001) in HLA-DR3 homozygous patients. In GD patients with ophthalmopathy (grade II or higher, according to Werner) DRB3*0101/*0202 heterozygosity revealed an increased relative risk of 5.5 (P〈0.001). Non-HLA-DR3 homozygous, DRB3*0101/*0202 heterozygous patients were at the highest risk for endocrine ophthalmopathy (relative risk 10; P〈0.001). Our data, based on DNA typing methods of HLA-D genes, provide evidence that the susceptibility is strongly associated with HLA-DRB3 genes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00180447
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 8
    Electronic Resource
    Electronic Resource
    Immunogenetic markers in patients with Graves' disease (1991)
    Springer
    Journal of molecular medicine 69 (1991), S. 256-260 
    Details
    ISSN: 1432-1440
    Keywords: Graves' disease ; HLA antigens ; Endocrine exophthalmos
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary 110 carefully characterized Caucasoid patients with Graves' disease were tested for HLA class I and class II antigens. Compared with Caucasian controls (n=193), the frequencies of HLA B8, Cw7 and DR3 were significantly increased (pc〈0.05). In the subgroups with and without exophthalmos, HLA A3 exhibited a negative but insignificant association with the eye involvement, while A19 and Cw2 showed positive, however even weaker correlations with eye disease. HLA DR5 was associated with relapsing thyrotoxicosis, whereas HLA DR7 and B12 were negatively correlated with relapse. These results confirm the positive correlation of HLA B8 and DR3 with Graves' disease and reveal a not yet observed association with Cw7. Reported correlations of antigen frequencies with eye disease and relapsing thyrotoxicosis could not be confirmed. Other previously unknown, however subtle differences in disease subgroups were observed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01666851
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 9
    Electronic Resource
    Electronic Resource
    Human coagulation factor XIIIA (FXIIIA) phenotyping by immunofixation agarose gel electrophoresis (IAGE) (1982)
    Springer
    Annals of hematology 44 (1982), S. 309-314 
    Details
    ISSN: 1432-0584
    Keywords: Coagulation factor XIII ; Genetic polymorphism ; Immunofixation electrophoresis ; Blood grouping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The genetic polymorphism of the A subunit of human coagulation factor XIII (FXIIIA) was investigated in platelet lysates from 239 blood donors from Hessen, Germany, and thereby for the first time in an European population. FXIIIA activity was detected by using a monospecific rabbit anti-human factor XIII subunit A antiserum for immunofixation after high voltage thin-layer agarose gel electrophoresis (IAGE). Three common phenotypes were observed (FXIII A 1, 2-1, and 2). The calculated allele frequencies were FXIIIA*1=0.797 and FXIIIA*2=0.203. The application of the FXIIIA polymorphism in blood group genetics is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00320706
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 10
    Electronic Resource
    Electronic Resource
    Book reviews (1988)
    Springer
    Annals of hematology 57 (1988), S. 6-6 
    Details
    ISSN: 1432-0584
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00320627
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...