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  • 1
    Electronic Resource
    Electronic Resource
    Somatic hybridization in the genus Solanum: S. tuberosum and S. brevidens (1984)
    Springer
    Plant cell reports 3 (1984), S. 165-167 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Somatic hybrid plants were regenerated from fused mesophyll protoplasts of an albino potato (Solanum tuberosum spp. tuberosum) variant and Solanum brevidens, a non-tuber bearing species which is sexually incompatible with S. tuberosum. These somatic hybrid plants represent the first example of direct hybridization between potato and members of the taxonomic group Etuberosa, and offer the potential for introgressing valuable germplasm from Solanum species outside the sexually compatible range into a worldwide crop species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00270215
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  • 2
    Electronic Resource
    Electronic Resource
    The effect of genotype on pollen transmission of mitochondria in rapeseed (Brassica napus) (1993)
    Springer
    Sexual plant reproduction 6 (1993), S. 33-39 
    Details
    ISSN: 1432-2145
    Keywords: Biparental/paternal/maternal inheritance ; Cytoplasmic male sterility ; Cybrids ; Organelles
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Crossing experiments were conducted to determine whether parental genotype affected the rate of transmission of paternal mitochondria to progeny in rapeseed (Brassica napus). Progeny were screened either by RFLP analysis of mitochondrial (mt) DNA or by means of a mt marker that causes male sterility. To date we have transferred paternal mitochondria to progeny in only cross, i.e. a specific female line crossed to a specific male line. The male line carries the polima cytoplasm, the mitochondria of which confer a characteristic malesterile flower morphology when in a napus nuclear background. This line is male fertile due to a restorer gene carried on an extra chromosome from a closely related species, Brassica juncea. The female line has a Brassica campestris cytoplasm with a chloroplast mutation conferring resistance to triazine herbicides. Progeny with mixtures of parental mtDNA display a range of plant phenotype from complete male fertility through varying proportions of male-sterile sectors to complete male sterility. The male sterility or fertility of flowers on a sector of a plant reflects the mt population of that sector, and such sectors will give rise to stably fertile or sterile progeny. These experiments suggest that maternal inheritance of mitochondria in higher plants is due to genes active in both the pollen parent and the egg parent.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00227580
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  • 3
    Electronic Resource
    Electronic Resource
    The introduction of CMS mitochondria to triazine tolerant Brassica napus L., var. “Regent”, by micromanipulation of individual heterokaryons (1986)
    Springer
    Plant cell reports 5 (1986), S. 415-418 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cytoplasmic triazine tolerance and cytoplasmic male sterility traits were combined in the nuclear genomic background of the Brassica napus variety ‘Regent’, following protoplast fusion, selection of fusion products by manual micro-manipulation, and culture in a Nicotiana tabacum nurse system. Whole plant cybrid regenerants were morphologically normal and produced seed on pollination, demonstrating their potential for incorporation into a breeding program.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00269630
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  • 4
    Electronic Resource
    Electronic Resource
    Mitochondrial DNA variation in races of maize indigenous to Mexico (1983)
    Springer
    Theoretical and applied genetics 65 (1983), S. 129-144 
    Details
    ISSN: 1432-2242
    Keywords: Zea mays ; Mexican races of maize ; Mitochondrial DNA ; Cytoplasmic male sterility
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Mitochondrial DNAs have been examined in accessions of 25 Mexican races of maize and compared with the mitochondrial DNAs previously found in inbred lines from the USA. Many variants were found. Low molecular weight DNA components, not previously found in US lines, were found in many of the accessions. Accessions classified as belonging to the same race, and plants from a single accession, sometimes had different mitochondrial genomes. Mitochondrial genomes similar to those in T and S cytoplasms were found in Mexican accessions. A low molecular weight linear DNA species has partial homology with a sequence in the high molecular weight mitochondrial genome. All plants with a shorter version of the linear molecule had a correspondingly altered region of homology in the high molecular weight genome. There is evidence that the geographical distribution of mitochondrial DNA types within Mexico is not random. One type, found in the oldest races, appears to be widely dispersed but another less common type appears to be confined largely to coastal regions. The potential value of these findings in maize breeding and for evolutionary studies is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00264880
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  • 5
    Electronic Resource
    Electronic Resource
    A rapid, single leaf, nucleic acid assay for determining the cytoplasmic organelle complement of rapeseed and related Brassica species (1987)
    Springer
    Theoretical and applied genetics 73 (1987), S. 364-370 
    Details
    ISSN: 1432-2242
    Keywords: Brassica ; Mitochondrial DNA (mtDNA) ; Chloroplast DNA (cpDNA) ; Cytoplasmic male sterility (cms) ; RFLP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary An assay is described whereby Eco RI restriction fragment length polymorphisms of mitochondrial and chloroplast DNAs can definitively identify cytoplasms of interest in Brassica crop development. Restrictable mitochondrial and chloroplast DNA is extracted from as little as 2–3 g and 0.5 g leaf tissue, respectively, and the donor plants are able to continue to develop in a normal manner. An unknown cytoplasm can be identified in three days, which is a considerable saving in time and labor compared to the several years required by traditional methods. The assay is very inexpensive and should be established as a routine procedure in laboratories involved in sexual or somatic Brassica hybrid production.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00262502
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  • 6
    Electronic Resource
    Electronic Resource
    Cytoplasmic DNA variation in a potato protoclonal population (1984)
    Springer
    Theoretical and applied genetics 69 (1984), S. 211-216 
    Details
    ISSN: 1432-2242
    Keywords: Potato ; Protoclones ; Mitochondrial DNA ; Chloroplast DNA ; Solanum tuberosum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Mitochondrial DNA variation was detected in potato plants (protoclones) regenerated from leaf mesophyll protoplasts. Two forms of variation were evident; (1) DNA sequence alterations within the high molecular weight mitochondrial chromosome and (2) the appearance of an additional low molecular weight mitochondrial DNA species. Variation in chloroplast DNA was not detected. The data suggests that protocloning can introduce molecular diversity into mitochondrial genomes and thereby assist in overcoming the cytoplasmic genetic uniformity prevalent in most major crops.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00272897
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  • 7
    Electronic Resource
    Electronic Resource
    Mitochondrial DNA rearrangements in somatic hybrids of Solanum tuberosum and Solanum brevidens (1986)
    Springer
    Theoretical and applied genetics 72 (1986), S. 787-793 
    Details
    ISSN: 1432-2242
    Keywords: Mitochondrial DNA ; Mitochondrial recombination ; Chloroplast DNA ; Solanum ; Somatic hybrids ; Protoplast fusion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Thirty somatic hybrids between Solanum tuberosum and Solanum brevidens were analysed for mitochondrial and chloroplast genome rearrangements. In all cases, the chloroplast genomes were inherited from one of the parental protoplast populations. No chloroplast DNA alterations were evident but a range of mitochondrial DNA alterations, from zero to extensive intra- and inter-molecular recombinations, were found. Such recombinations involved specific ‘recombination hot spots’ in the mitochondrial genome. Not all hybrids regenerated from a common callus possessed identical mitochondrial genomes, suggesting that sorting out of mitochondrial populations in the callus may have been incomplete at the plant regeneration stage. Sorting out of organelles in planta was not observed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00266546
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  • 8
    Electronic Resource
    Electronic Resource
    A rapid, single leaf assay for detecting the presence of ‘S’-male-sterile cytoplasm in maize (1980)
    Springer
    Theoretical and applied genetics 57 (1980), S. 97-100 
    Details
    ISSN: 1432-2242
    Keywords: Zea mays ; Male-sterility ; ‘S’-Cytoplasm ; Mitochondria ; DNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A simple, rapid, and reproducible assay is described for determining unambiguously the presence of S-type cytoplasm in male-sterile and male-fertile (restored) maize lines. Because the assay requires only 0.5 g leaf segment per sample, it is a single plant assay and the plant is not destroyed. Plants at any developmental stage can be used. The assay involves a 30 sec homogenization, 20 min centrifugation, one hour lysis, overnight agarose electrophoresis, 30 min gel staining, and photography of the gel to produce a result in much less than 24 hr. Many samples can be assayed simultaneously. The various assay methods available for classifying maize cytoplasms are compared and discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00253876
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  • 9
    Electronic Resource
    Electronic Resource
    Mitochondrial DNA analyses of fertile and sterile maize plants derived from tissue culture with the texas male sterile cytoplasm (1982)
    Springer
    Theoretical and applied genetics 62 (1982), S. 213-217 
    Details
    ISSN: 1432-2242
    Keywords: Maize tissue culture ; Drechslera maydis race T toxin ; Texas male-sterility ; Mitochondrial DNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Maize plants carrying Texas (T) cytoplasm are male-sterile and sensitive to Drechslera maydis race T toxin, whereas plants carrying Normal (N) cytoplasm are male-fertile and resistant to the toxin. Some plants regenerated from T cytoplasm tissue cultures exhibit a N cytoplasm-like phenotype with respect to malefertility and toxin-resistance. Analysis of the high molecular weight mitochondrial DNA (mtDNA) of such regenerants has shown that the plants do not contain N cytoplasm mtDNA. However, their mtDNAs do show sequence differences from each other and from the mtDNA of plants possessing T cytoplasm. No single alteration detected correlates with the change to malefertility or toxin resistance. Sequence alterations were also evident in high molecular weight mtDNA isolated from a plant regenerated from N cytoplasm callus. No changes in low molecular weight mtDNA molecules were observed in regenerants from N or T cytoplasm callus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00276241
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  • 10
    Electronic Resource
    Electronic Resource
    The combination of polima cms and cytoplasmic triazine resistance in Brassica napus (1987)
    Springer
    Theoretical and applied genetics 73 (1987), S. 809-814 
    Details
    ISSN: 1432-2242
    Keywords: B. napus ; Protoplast fusion ; Cybrids ; Triazine resistance ; Male sterility
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Protoplast fusion was used to combine cytoplasmic triazine resistance (ctr) and Polima type cytoplasmic male sterility (cms) in Brassica napus. The cybrids produced constitute the major biological input required for the production of commercial single-cross hybrid rapeseed bearing cytoplasmic triazine resistance. The results also indicate that Polima cms is associated with the mitochondrial genome.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00289383
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