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  • 1
    Electronic Resource
    Electronic Resource
    Identification of fetal blood stains by radioimmunoassay of α1-fetoprotein (1979)
    Springer
    International journal of legal medicine 82 (1979), S. 323-326 
    Details
    ISSN: 1437-1596
    Keywords: Fetal blood stains, Radioimmunoassay of α1-Fetoprotein ; α1-Fetoprotein, fetal blood stains ; Spurenkunde, α1-Fetoprotein ; Blutspuren, Fetalblut ; Fetalblut, Nachweis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Law
    Description / Table of Contents: Zusammenfassung Es wird die radioimmunologische Bestimmung von α1-Fetoprotein(AFP) zum forensischen Nachweis von Fetalblutspuren unter Verwertung eines kommerziellen Bestimmungsansatzes beschrieben. Der Gehalt von AFP in Fetalblutspuren auf Filterpapier betrug 21–320 ng/mm2. In Spuren von Erwachsenenblut sowie Retroplazentarblut konnte das Protein nur in äußerst geringfügigen Konzentrationen nachgewiesen werden. Das Spurenalter beeinflußte die Bestimmung bis zu 1 Monat nicht. Die Methode ist einfach und empfindlich genug für die gerichtsmedizinische Praxis.
    Notes: Summary Radioimmunoassay of α1-fetoprotein(AFP) for medico-legal identification of fetal blood stains using a commercial kit is described. The AFP content in fetal blood stains on filter paper ranged from 21 – 320 ng/9 mm2. The protein was detected in stains of adult blood and retroplacental blood in only negligible amounts. Aging of the blood stains did not influence the values up to 1 month. The method is simple and sensitive enough for application to medico-legal-practice.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02092044
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  • 2
    Electronic Resource
    Electronic Resource
    Delay in administration of CDDP until completion of AGM-1470 treatment enhances antimetastatic and antitumor effects (1999)
    Springer
    Clinical & experimental metastasis 17 (1999), S. 19-23 
    Details
    ISSN: 1573-7276
    Keywords: AGM-1470 (TNP-470) ; CDDP ; combination therapy ; metastasis ; rat osteosarcoma
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The efficacy of cis-diammine dichloroplatinum (CDDP) therapy in combination with continuous administration of angiogenesis inhibitor o-(chloroacetyl-carbamoyl) fumagillol (AGM-1470) was evaluated experimentally using a transplantable rat osteosarcoma line previously established in our laboratory. AGM-1470 (2.5 mg/kg body weight/week) was administered by Alzet osmotic pumps for 2 weeks starting from 7 days after tumor inplantation and CDDP (1.25 mg/kg) was given on days 21 and 24. The number of lung metastatic nodules was counted and the wet weights of the primary tumors were measured 5 weeks after tumor inplantation. Values with administration of CDDP 3 days after discontinuation of AGM-1470 were significantly lower than when the two agents were coadministered (P〈0.05). This animal model should facilitate optimization of the timing of combination therapy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1026445024899
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  • 3
    Electronic Resource
    Electronic Resource
    Possible contribution of aminopeptidase N (APN/CD13) to invasive potential enhanced by interleukin-6 and soluble interleukin-6 receptor in human osteosarcoma cell lines (1999)
    Springer
    Clinical & experimental metastasis 17 (1999), S. 857-863 
    Details
    ISSN: 1573-7276
    Keywords: aminopeptidase N ; interleukin-6 ; invasion ; matrigel ; osteosarcoma
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract This study aimed at clarifying the role of Aminopeptidase N (APN), a Zn2+-dependent ectopeptidase localized on the cell surface of human osteosarcoma cell lines treated with proinflammatory cytokines. We investigated the proinflammatory cytokines interleukin-1 beta (IL-1β), IL-6 and tumor necrosis factor alpha (TNF-α) as well as the anti-inflammatory cytokine transforming growth factor beta (TGF-β) for their influence on APN regulation. Soluble IL-6 receptor (sIL-6R) was always used together with IL-6 to achieve a stable effect. In addition, the invasive potential of the osteosarcoma cell lines MG63 and HOS was examined. Competitive RT-PCR and Ala-pNA activity assays revealed that IL-6 and sIL-6R significantly increased the mRNA expression and activity of APN in both osteosarcoma cell lines. Although IL-1β significantly stimulated APN mRNA expression in both cell lines, it influenced the enzyme activity only in MG63. TNF-α and TGF-β, however, had an effect neither on mRNA expression nor on the enzyme activity of APN in both cell lines. In the Matrigel invasion assay, IL-6 and sIL-6R significantly up-regulated the transmigration of these cell lines, whereas other cytokines did not. The up-regulated invasion was inhibited by bestatin, a specific inhibitor of APN. Cellular migration correlated highly with APN activity (r = 0.79, P 〈 0.002). These findings suggest that APN contributes to the invasive potential of human osteosarcomas enhanced by IL-6 and SIL-6R.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006794617406
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  • 4
    Electronic Resource
    Electronic Resource
    Group-specific component subtyping in semen and seminal stains by conventional isoelectric focusing (1995)
    Weinheim : Wiley-Blackwell
    Electrophoresis 16 (1995), S. 1024-1026 
    Details
    ISSN: 0173-0835
    Keywords: Group-specific component subtyping ; Semen ; Seminal stains ; Isoelectric focusing ; Neuraminidase treatement ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Group-specific component (GC) subtyping in semen and seminal stains was carried out using isoelectric focusing in carrier ampholyte-generated pH gradients and immunoblotting. In serum samples the anodal bands of GC 1F and of GC 1S disappeared by neuraminidase treatment, but in semen samples these bands remained unchanged after such treatment. The GC 2 type in semen exhibited two bands: the main GC 2 band and another fast band which focused at the position of the cathodic band of GC 1F. These seminal GC bands were unaffected by enzyme digestion. Reliable subtyping was possible in seminal stains stored at 4°C for up to 10 weeks, at room temperature for up to 8 weeks, and at 37°C for up to 5 weeks. The GC subtyping by conventional isoelectric focusing after neuraminidase treatment is simple, economical and useful in medicolegal examination of seminal stains.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.11501601172
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  • 5
    Electronic Resource
    Electronic Resource
    Alternative method of subtyping the A subunit of coagulation factor XIII (FXIIIA) (1997)
    Weinheim : Wiley-Blackwell
    Electrophoresis 18 (1997), S. 790-791 
    Details
    ISSN: 0173-0835
    Keywords: Factor XIIIA subtyping ; Isoelectric focusing ; Polymorphism ; Japanese ; Chinese ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: An alternative isoelectric focusing method for the A subunit of coagulation factor XIII (FXIIIA) subtyping is described which employs three different carrier ampholytes pH 4-6.5, pH 5-6 and pH 5-8 (2:2:1), and sample application at the anode. With this technique ten common subtypes, including a variant, were clearly and reliably identified in Japanese and Chinese population samples.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/elps.1150180522
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