ISSN:
1435-0653
Source:
Springer Online Journal Archives 1860-2000
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
Notes:
Triticum aestivum L.) from the Pacific Northwest (PNW) were used to compare pedigree and amplified fragment length polymorphism (AFLP)-based genetic diversity estimates (GDEPED and GDEAFLP, respectively). The mean of the 903 GDEPED pairwise comparisons was 0.96, and 89% of the values were 〉0.90. In contrast, GDEAFLP values were normally distributed (mean = 0.54), suggesting that this diversity measure was better able to identify pairs of genotypes representing the entire range of possible GDEs. Thus, GDEAFLP may have more utility than GDEPED for identifying parental combinations with maximum allelic variation. Despite the substantially different means and distributions of the two diversity measures, a moderate rank correlation (r s = 0.42, P 〈 0.001) was detected between the two GDE matrices. AFLP fragments from hypomethylated portions of the genome (generated with Pst I:MseI) were more highly associated with GDEPED than were fragments generated with the methylation insensitive combination EcoRI:MseI (r s = 0.44 and 0.28, respectively). Pedigree and AFLP-based GDEs detected a similar hierarchical pattern of genetic diversity among the 43 cultivars evaluated. Although GDEPED was not well suited for identifying degree of relatedness among individuals, it may be adequate for assessing overall patterns of genetic variation among regionally adapted germplasm. It remains to be determined which method, if either, provides predictive estimates of heterosis or genetic variance among progeny from specific parental combinations.
Type of Medium:
Electronic Resource
Permalink
