ISSN:
1617-4623
Keywords:
Aspergillus oryzae
;
Transformation
;
A. niger pyrG gene
;
Cotransformation
;
Phleomycin
;
Escherichia coli lacZ gene
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary A transformation system for Aspergillus oryzae based on the orotidine-5′-phosphate decarboxylase gene (pyrG) was developed. Transformation frequencies of up to 16 transformants per μg of DNA were obtained with the vector pAB4-1, which carries the pyrG gene of A. niger. Southern blotting analysis showed that vector DNA sequences were integrated into the chromosomal DNA, in various copy numbers and presumably at different sites. Efficient cotransformation of an unselectable gene was also shown. Under the conditions used no transformants were obtained with the equivalent pyr4 gene of Neurospora crassa.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00327197
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