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  • 1
    Electronic Resource
    Electronic Resource
    The pigments of the gliding bacterium Herpetosiphon giganteus (1978)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 3 (1978), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1978.tb01906.x
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  • 2
    Electronic Resource
    Electronic Resource
    β-Carotene synthesis in isolated chromoplasts from Narcissus pseudonarcissus (1980)
    Springer
    Planta 150 (1980), S. 435-438 
    Details
    ISSN: 1432-2048
    Keywords: Carotene synthesis ; Chromoplasts ; Narcissus ; Polyprenoids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A system has been established from isolated intact chromoplasts of Narcissus pseudonarcissus flowers that synthesizes geranylgeraniol, an unknown polyprenoid alcohol, phytoene, and β-carotene from [1-14C]isopentenyl pyrophosphate in a good yeild. Long chain pyrophosphates are not accumulated. San 6706 inhibits the dehydrogenation of phytoene, whereas nicotine does not lead to an accumulation of lycopene. Separation and identification of polyprenoid lipids was performed by HPLC. The properties and advantages of the chromoplast system are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00390182
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  • 3
    Electronic Resource
    Electronic Resource
    On the compartmentation of isopentenyl diphosphate synthesis and utilization in plant cells (1981)
    Springer
    Planta 153 (1981), S. 578 
    Details
    ISSN: 1432-2048
    Keywords: Chloroplasts ; Chromoplasts ; Isopentenyl diphosphate ; Narcissus ; Polyprenoids ; Spinacia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Purified spinach chloroplast and daffodil chromoplast preparations do not use mevalonate, phosphomevalonate, and diphosphomevalonate for the synthesis of isopentenyl diphosphate. Isopentenyl diphosphate, on the other hand, is incorporated into plastidal polyprenoids in large amounts. In the presence of a cytoplasmic supernatant, however, mevalonate and the phosphomevalonates were incorporated into the plastidal polyprenoids in equally large amounts, which demonstrates that the enzymes mevalonate kinase (EC 2.7.1.36), phosphomevalonate kinase (EC 2.7.4.2), and diphosphomevalonate decarboxylase (EC 4.1.1.33) are soluble cytoplasmic enzymes and that they apparently do not occur as isoenzymes within the plastids. The concept is developed that isopentenyl diphosphate is a central intermediate in plant polyprenoid formation which is channeled into several compartment for different biosynthetic pathways.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00385544
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Phytoene synthase and phytoene dehydrogenase associated with envelope membranes from spinach chloroplasts (1982)
    Springer
    Planta 156 (1982), S. 176-180 
    Details
    ISSN: 1432-2048
    Keywords: Carotenoid biosynthesis ; Chloroplast envelope ; Phytoene dehydrogenase ; Phytoene synthase ; Spinacia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Envelope membranes of spinach chloroplasts contain appreciable activities of the carotenogenic enzymes phytoene synthase (formation of phytoene by condensation of two molecules geranylgeranyl pyrophosphate) and phytoene dehydrogenase (formation of lycopene from phytoene), plus a phosphatase activity. These results were obtained by coincubation experiments using isolated envelope membranes and either a phytoene-forming in vitro system (from [1-14C]isopentenyl pyrophosphate) or [14C]geranylgeranyl pyrophosphate or a geranylgeranyl-pyrophosphate-forming in vitro system (from [1-14C]isopentenyl pyrophosphate). Within thylakoids carotenogenic enzymes could not be detected. It is concluded that the chloroplast envelope is at least a principal site of the membrane-bound steps of carotenoid biosynthesis in chloroplasts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00395433
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  • 5
    Electronic Resource
    Electronic Resource
    Chlorophyll synthetase in chlorophyll-free chromoplasts (1981)
    Springer
    Plant cell reports 1 (1981), S. 40-42 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A considerable incorporation of [1-14C]isopentenyl diphosphate into chlorophyll in chromoplast preparations from daffodil flowers (Narcissus pseudonarcissus L.) was observed when exogenous chlorophyllide a was added. The enzyme chlorophyll synthetase showed properties of a peripheral membrane protein.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00267657
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  • 6
    Electronic Resource
    Electronic Resource
    Morphometric measurements of Daucus carota suspension culture cells (1977)
    Springer
    Planta 137 (1977), S. 85-87 
    Details
    ISSN: 1432-2048
    Keywords: Cell culture ; Daucus ; Membrane systems ; Morphometry ; Particle number ; Surface density
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Standard stereologic methods have been applied to electron micrographs of Daucus carota L. suspension culture cells. The relative frequencies of the different membrane systems within the cells have been determined and compared with published data form mature leaf cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00394440
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  • 7
    Electronic Resource
    Electronic Resource
    Oleosomes (Spherosomes) from Daucus carota suspension culture cells (1978)
    Springer
    Planta 140 (1978), S. 233-237 
    Details
    ISSN: 1432-2048
    Keywords: Acyl-CoA hydrolase ; Cell culture ; Daucus ; Oleosomes ; Triacylglycerol turnover
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Isolated oleosomes from Daucus carota L. cells are lipid droplets consisting mainly of triacylglycerols (〉97%) and very little protein (1–2%). The boundary between the lipid phase and the cytosol, which is visible on electron micrographs, is not built up by a true phospholipid-containing unit or half unit membrane. Enzymatic activities of lipid metabolism were not found to be associated with oleosomes with the exception of very low (contaminating) acyl-CoA:1,2-diacylglycerol acyltransferase (EC 2.3.1.20) and relatively high acyl-CoA hydrolase (EC 3.1.2.2) activities. The triacylglycerols exhibited a half life time of about 70 h, which is below the generation time of the cells (80–90 h). The fatty acid pattern of triacylglycerols was very similar to that of polar cellular membrane lipids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00390253
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  • 8
    Electronic Resource
    Electronic Resource
    Fatty acid and glycerolipid synthesis in abscised daffodil flowers after acetate feeding (1979)
    Springer
    Planta 144 (1979), S. 473-477 
    Details
    ISSN: 1432-2048
    Keywords: Fatty acid synthesis ; Flower tissue ; Lipid synthesis ; Narcissus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The coronae of Narcissus pseudonarcissus flowers incorporated [1-14C]acetate almost exclusively into the fatty acid moieties of glycerolipids. After a 4 h incubation, the newly synthesized acids were: stearate plus palmitate (50%); oleate (17%); linoleate (32%); and linolenate (0.5%). Phosphatidylcholine and diacylglycerol were the principal labelled lipids. In pulse experiments these acids were further desaturated, with time, to an appreciable extent and, concurrently, transferred essentially from phosphatidylcholine to diacylglycerol, diacylgalactosylglycerol, and diacylgalabiosylglycerol. The labelling of diacylgalactosylglycerol and diacylgalabiosylglycerol paralleled the appearance of linolenate. The distribution of labelled acids in phosphatidylcholine, diacylgalactosylglycerol, and diacylgalabiosylglycerol was very different. The results were compared with those obtained in vitro with isolated coronae chromoplasts and discussed in relation to current schemes of fatty acid and glycerolipid synthesis in plant cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00380125
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  • 9
    Electronic Resource
    Electronic Resource
    Galactolipid synthesis in chromoplasts in vitro (1979)
    Springer
    Planta 144 (1979), S. 467-471 
    Details
    ISSN: 1432-2048
    Keywords: Acyltransferase ; Chromoplasts ; Diacylglycerol galactosyltransferase ; Galactolipid synthesis ; Narcissus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Isolated chromoplasts from Narcissus pseudonarcissus flowers contain: a fatty acid synthesizing system; acyl-CoA synthetase (EC 6.2.1.3); glycero-phosphate acyltransferase (EC 2.3.1.15); acylglycero-phosphate acyltransferase; phosphatidate phosphatase (EC 3.1.3.4); diacylglycerol galactosyltransferase (EC 2.4.1.46); and diacylgalactosylglycerol galactosyltransferase, i.e. all enzymatic activities necessary for the synthesis of diacylgalactosylglycerol and diacylgalabiosylglycerol from acetate, HCO - 3 , sn-glycerol 3-phosphate, and UDP-d-galactose. Diacylgalactosylglycerol and diacylgalabiosylglycerol, however, are synthesized from these precursors to only a very low extent in an in vitro system. This is attributed to a specificity of diacylglycerol galactosyltransferase for highly unsaturated diacylglycerols. Specificities of acyltransferase reactions were also found.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00380124
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  • 10
    Electronic Resource
    Electronic Resource
    Phosphate translocator and adenylate translocator in chromoplast membranes (1980)
    Springer
    Planta 150 (1980), S. 170-173 
    Details
    ISSN: 1432-2048
    Keywords: Adenylate translocator ; Chromoplasts ; Fatty acid synthesis ; Narcissus ; Phosphate translocator
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract It is shown by the criteria of saturation kinetics, specificity, and inhibition experiments that chromoplast membranes from the daffodil flower contain a phosphate translocator for the counter-exchange of phosphate, and 3-phosphoglycerate, as well as phosphoenolpyruvate; they also contain an adenylate translocator. This is the first report on the occurrence of these translocators in non-green plastids. Both translocators exhibit certain dissimilar properties when compared to the corresponding systems of chloroplasts. The transport rates of both translocators are sufficient to allow a prominent fatty acid synthesis in isolated chromoplasts when C3 intermediates of the glycolytic pathway or adenine nucleotides are used as energy sources.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00582362
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