ISSN:
1617-4623
Keywords:
Key words Fission yeast
;
Checkpoint
;
Cell cycle
;
Hus1
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The structure of hus1 +, a Schizosaccharomyces pombe gene required for S-M and DNA damage checkpoints, has been determined. Expression of hus1 + requires splicing of five exons, including a microexon that is only 13 nucleotides long. hus1 + is predicted to encode a 33 kDa protein with no similarity to sequences in any database, including the entire S. cerevisiae genome. Yeast strains disrupted for the hus1 + gene are viable but checkpoint-defective. Polyclonal antibodies were raised against bacterially expressed Hus1 protein, and used to study Hus1 regulation. Hus1 protein levels are not affected by S-phase arrest, and are not altered by mutations in other checkpoint genes, suggesting that Hus1 is not regulated at the transcriptional or translational levels.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/PL00008606
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