ISSN:
0449-2978
Keywords:
Physics
;
Polymer and Materials Science
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
,
Physics
Notes:
Gel permeation chromatography is an elution chromatographic process depending on the permeation of the solute through a bed of gel particles. This process is used in the estimation of molecular weight distributions of polymers, since elution occurs in decreasing order of molecular size. The eluting species, however, are not perfectly fractionated, and apparent broadening of the distribution occurs. This broadening results from an axial (longitudinal) mixing of the eluting species. Consideration of the accessible bed volume for each species permits a correction to be made for this axial dispersion. The concept was applied to heterodisperse distributions by solving the resulting simultaneous equations. A least-squares regression may be employed to utilize the experimental data most effectively. The experimental chromatogram can be described in terms of accessible bed volume and dispersion coefficient of each species together with flow rate, sample concentration, and chromatograph column geometry. The chromatogram corrected for the axial dispersion describes the molecular weight distribution more accurately than does the experimentally determined curve. The correction procedure was applied to a well-characterized polystyrene; the results of the gel permeation chromatography show excellent confirmation of the results of fractionation and of other instrumental analyses.
Additional Material:
4 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/pol.1966.160040506
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