Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    Enkephalins Prouduced in Transgenic Plants Using Modified 2S Seed Storage Proteins (1989)
    [s.l.] : Nature Publishing Company
    Nature biotechnology 7 (1989), S. 929-932 
    Details
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] We have explored the possibility of producing large amounts of biologically active peptides as part of chimeric plant seed storage proteins, the 2S albumins. A portion of an Arabidopsis thaliana 2S albumin gene, encoding a region of the protein whose sequence is not highly conserved among different ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nbt0989-929
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Four genes in two diverged subfamilies encode the ribulose-1,5-bisphosphate carboxylase small subunit polypeptides of Arabidopsis thaliana (1988)
    Springer
    Plant molecular biology 11 (1988), S. 745-759 
    Details
    ISSN: 1573-5028
    Keywords: Arabidopsis thaliana ; multigene family ; ribulose-1,5-bisphosphate carboxylase ; sequence ; small subunit
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The multigene family encoding the small subunit polypeptides of ribulose-1,5-bisphosphate carboxylase/oxygenase in the crucifer Arabidopsis thaliana has been isolated and the organization and structure of the individual members determined. The family consists of four genes which have been divided into two subfamilies on the basis of linkage and DNA and amino acid sequence similarities. Three of the genes, designated ats1B, ats2B, and ats3B, reside in tandem on an 8 kb stretch of the chromosome. These genes share greater than 95% similarity in DNA sequence and encode polypeptides identical in length and 96.7% similar in amino acid sequence. The fourth gene, ats1A, is at least 10 kb removed from, or completely unlinked to the B subfamily. The B subfamily genes are more similar to each other than to ats1A in nucleotide and amino acid sequence. All four genes are interupted by two introns whose placement within the coding region of the genes is conserved. The introns of the B subfamily genes are similar in length and nucleotide sequence, but show no similarity to the introns of ats1A. Comparison of the DNA sequences within the immediate 5′ and 3′ flanking sequences among the genes revealed only limited regions of homology. S1 analysis shows that all four genes are expressed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00019515
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Unexpectedly higher expression levels of a chimeric 2S albumin seed protein transgene from a tandem array construct (1994)
    Springer
    Plant molecular biology 26 (1994), S. 1001-1005 
    Details
    ISSN: 1573-5028
    Keywords: 25 albumin ; Arabidopsis ; Brazil nut ; post-transcriptional regulation ; tandem genes ; transgenic plant
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In wild-type Arabidopsis seeds the 2S albumin seed protein gene family members are differentially expressed. In this work it is shown that as predicted by the wild type situation, the at2S2 promoter is much more effective than that of the at2S1 gene in the expression of a transgene. However, unexpectedly high expression levels were obtained using a construct in which the transgene was present as a tandem duplication in the T-DNA. Neither in this case nor in homozygous plants with either construct was epigenetic silencing observed. While transgene mRNA levels were of the same order of magnitude as the endogenous at 2S2 gene, protein levels were much lower.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00028867
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    DNA sequences for the Zea mays tRNA genes tV-UAC and tS-UGA: tV-UAC contains a large intron (1984)
    Springer
    Plant molecular biology 3 (1984), S. 13-20 
    Details
    ISSN: 1573-5028
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The chloroplast genome contains genes for a large and probably complete set of tRNAs. These genes are unique in sharing attributes of both nuclear and bacterial tRNA genes. Two chloroplast tRNA genes from Zea mays are described here. tV-UAC, encoding a valine tRNA with the anticodon UAC, contains a 603 bp intron and is highly homologous, both in coding regions and in the intron, to the analogous gene from tobacco described by Deno et al. (Nucleic Acids Res 10:7511–7520, 1982). It is located near the gene for the beta and epsilon subunits of the CF1 complex. (Krebbers et al.: Nucleic Acids Res 10:4985–5002, 1982). The gene tS-UGA, encoding a serine tRNA with the anticodon UGA, is located 41 kbp 3′ to tV-UAC. Both genes contain promoter-like sequences in their 5′ flanking regions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00023411
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    Aspartic proteinase genes in the Brassicaceae Arabidopsis thaliana and Brassica napus (1997)
    Springer
    Plant molecular biology 33 (1997), S. 187-192 
    Details
    ISSN: 1573-5028
    Keywords: aspartic proteinase ; gene isolation ; intron/exon arrangement ; protein processing ; pulmonary surfactant-associated protein ; saposin C
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Active aspartic proteinase is isolated from Brassica napus seeds and the peptide sequence is used to generate primers for PCR. We present here cDNA and genomic clones for aspartic proteinases from the closely related Brassicaceae Arabidopsis thaliana and Brassica napus. The Arabidopsis cDNA represents a single gene, while Brassica has at least 4 genes. Like other plant aspartic proteases, the two Brassicaceae enzymes contain an extra protein domain of about 100 amino acids relative to the mammalian forms. The intron/exon arrangement in the Brassica genomic clone is significantly different from that in mammalian genes. As the proteinase is isolated from seeds, the same tissue where 2S albumins are processed, this implies expression of one of the aspartic proteinase genes there.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1005794917200
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    A trout growth hormone is expressed, correctly folded and partially glycosylated in the leaves but not the seeds of transgenic plants (1994)
    Springer
    Transgenic research 3 (1994), S. 304-310 
    Details
    ISSN: 1573-9368
    Keywords: trout growth hormone ; transgenic plants ; tissue-specific expression ; disulphide bridge formation ; glycosylation ; SSU promoter ; at2S1 promoter ; Arabidopsis thaliana ; Nicotiana tabacum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The growth hormone gene of the rainbow trout (tGH-II) was expressed in leaves of transgenic tobacco plants and seeds of transgenicArabidopsis plants using tissue-specific promoters. Although in the leaves and the seeds comparble amounts of tGH-II mRNA could be detected, the protein could only be identified in the tobacco leaves. Passage of the hormone into the secretory pathway, mediated by the signal sequence of the extracellular tobacco PRI-b (pathogenesis-related) protein, resulted in correct disulphide bridge formation and (partial) glycosylation of the hormone. In contrast, cytoplasmic expression resulted in misfolding and partial breakdown of the protein. The data demonstrate that synthesis, folding and glycosylation of heterologous proteins in plants is dependent both on subcellular location as well as on the tissue or cell type in which the protein is expressed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01973590
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 7
    Electronic Resource
    Electronic Resource
    The paramutagenic line niv-44 has a 5 kb insert, Tam 2, in the chalcone synthase gene of Antirrhinum majus (1985)
    Springer
    Molecular genetics and genomics 199 (1985), S. 201-207 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Several alleles of the nivea locus of Antirrhinum majus, both stable and unstable, have been characterised genetically (Harrison and Carpenter 1973 a, b). In this work the niv-44 allele is characterised at the molecular level. It contains a 5kb insertion element, Tam 2, which has 14 base pair inverted repeats. There is a three base pair duplication at the target site, which is at the first intron-exon boundary of the chalcone synthase gene. Tam 2 homologous sequences are present in multiple copies in several A. majus lines, including niv-53, and most have at least a 2.9 kb sequence in common with the copy at the chalcone synthase gene. Possible reasons for the apparent stability of the niv-44 allele and molecular explanations for the role of this allele in paramutation in A. majus are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00330260
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 8
    Electronic Resource
    Electronic Resource
    Isolation of homozygous transgenicBrassica napus lines carrying a seed-specific chimeric 2S albumin gene and determination of linkage relationships (1995)
    Springer
    Molecular breeding 1 (1995), S. 143-153 
    Details
    ISSN: 1572-9788
    Keywords: 2S Albumin ; Brassica napus ; chimeric seed storage protein gene ; co-transformation ; T-DNA insertion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract UsingAgrobacterium tumefaciens-mediated gene transfer, 14 T0Brassica napus plants carrying one of three chimeric 2S albumin seed protein genes were obtained after co-transformation with theneo gene. Plants were made homozygous using either cell haploid culture or, for single-copy plants, traditional crossing methods. Sixty-five percent of kanamycin-resistant plants contained at least one copy of the seed protein gene, and multiple copies were usually at a single locus. In the majority of cases, at least one copy of theneo gene was linked to an introduced 2S albumin gene, demonstrating that co-transformation is not a reliable way to obtain lines without the marker gene linked to the gene of interest. In 3 T0 plants sequences derived from beyond the left border of the vector were integrated in the plant genome, in two cases partially rearranged, confirming that T-DNA insertion is not always precise. Procedures for efficiently determining this are described. This work highlights the extra steps needed to prepare transgenic lines for field studies and potential further breeding.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01249699
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 9
    Electronic Resource
    Electronic Resource
    Expression of the 2S albumin from Bertholletia excelsa in Brassica napus (1990)
    Springer
    Molecular genetics and genomics 221 (1990), S. 306-314 
    Details
    ISSN: 1617-4623
    Keywords: Brazil nut ; Brassica napus ; 2S albumin ; Transgenic plants ; Tissue specific expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The methionine rich 2S albumin seed storage protein of Bertholletia excelsa has been expressed in seeds of Brassica napus (rapeseed). A chimeric gene driven by the soybean lectin 5′ flanking regions was used to produce a fusion protein consisting of the soybean lectin signal peptide and the propeptide of the Brazil nut 2S albumin. Several transgenic plants were studied at the RNA and protein levels; in each case the chimeric gene was expressed and the protein detected at levels ranging from 0.02% to 0.06% of total protein. Transcriptional studies in a particular transgenic plant show that expression of the gene is tissue specific and developmentally regulated during seed maturation. The endogenous napin genes and the introduced gene are regulated differently, with expression of the chimeric gene paralleling that seen when the soybean lectin gene is expressed in other plant species. Western analysis using antibodies to Brazil nut 2S albumins resulted in the detection of a protein whose size is consistent with correct processing of the precursor.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00259393
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 10
    Electronic Resource
    Electronic Resource
    Molecular analysis of paramutant plants of Antirrhinum majus and the involvement of transposable elements (1987)
    Springer
    Molecular genetics and genomics 209 (1987), S. 499-507 
    Details
    ISSN: 1617-4623
    Keywords: Tam1 ; Tam2 ; Paramutation ; DNA rearrangement
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Paramutation is observed when the Antirrhinum majus lines 44 and 53 are crossed. These two lines both have insertions at the nivea locus, which encodes chalcone synthase (chs). The allele niv-53 carries the transposable element Tam1 in the promoter region of the chs gene; niv-44 carries the element Tam2 within the gene. The Tam1 element has previously been extensively characterised. Here the Tam2 element is further characterised, and the arrangement of the nivea locus in paramutant plants is analysed. The complete sequence of Tam2, and that of a partial cDNA complementary to it, have been determined. The cDNA is probably transcribed from a different copy of Tam2 from that present at the nivea locus, and does not encode a functional protein. Genomic Southerns of F1 plants from the 53/44 cross show that no major rearrangements are consistently associated with paramutation at the nivea locus of A. majus. The isolation from a paramutant plant arising from a 53/44 cross of an allele (niv-4432) resulting from the excision of Tam2 is reported. The excision of Tam2 resulted in a 32 bp deletion of chs gene sequences. Plants homozygous for the new niv-4432 allele have white flowers and are still paramutagenic, demonstrating that Tam2 need not be present at the nivea locus for paramutation to occur. Different interactions between Tam1 and Tam2 are discussed, and a possible model for paramutation is presented.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00331156
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...