ISSN:
1617-4623
Keywords:
Antirrhinum majus
;
DNA sequences
;
Nivea locus
;
Transposable elements
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Two stable derivatives of the highly unstable niv-53::Tam1 allele of Antirrhinum majus were analysed. In both derivatives the Tam1 element is integrated at the same site and in the same orientation as in the parental niv-53::Tam1 allele. In both cases the Tam1 element was found to carry a 5 bp deletion (CACTA) in one of its termini. This explains the excision deficiency of these two alleles of Tam1, niv-53::Tam1-46 and niv-53::Tam1-49. Niv-44::Tam2, another stable nivea mutation, carries the 5 kb element Tam2, which is not a derivative of Tam1 but possesses identical terminal inverted repeats. When the stable lines 46 and 49 were corssed with line 44, suprisingly, a high number of the flowers in the F1 displayed a variegated phenotype. Sequence analysis of two germinal revertants isolated from the heterozygote niv-53::Tam1-46/niv-44::Tam2 shows excision of the Tam2 element. This indicates that Tam2 is a defective element, which can be complemented by an active Tam1 element. However, the variegated F1 phenotype observed is not inherited monofactorially. Variegation is seen only at particular times of development of the F1 plants. These phenomena seem to involve both the Tam1 and Tam2 transposable elements.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00331489
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