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DEVELOPMENTAL REGULATION OF THE EXPRESSION OF ALCOHOL DEHYDROGENASE IN RIPENING TOMATO FRUITS (1990)

LONGHURST, T. J. ; TUNG, H. F. ; BRADY, C. J.

Oxford, UK : Blackwell Publishing Ltd

Journal of food biochemistry 14 (1990), S. 0

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ISSN: 1745-4514

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Process Engineering, Biotechnology, Nutrition Technology

Notes: In each of 5 tomato (Lycopersicon esculentum Mill) cultivars examined, alcohol dehydrogenase (E.C.1.1.1.1) specific activity decreased during the early stages of ripening and then increased in the postclimacteric period. Alcohol dehydrogenase specific activity also increased when immature, mature or pink fruit were placed in an atmosphere of 3% (V/V) oxygen. Measurements of oxygen in the internal tissues and the respiratory quotient throughout ripening established that fruit ripening in air does not suffer an oxygen stress. Increases in alcohol dehydrogenase activity were also observed in pink fruit exposed to 10% (V/V) carbon dioxide. Such atmospheres are known to result in a lowering of the cytoplasmic pH in plants and it is suggested that alcohol dehydrogenase activity is induced during ripening in response to cytoplasmic acid stress. The increased ADH activity during ripening may contribute to flavor development.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1111/j.1745-4514.1990.tb00804.x

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Full-length cDNA sequence of X-linked G6PD of an Australian marsupial, the wallaroo (1995)

Loebel, D. A. F. ; Longhurst, T. J. ; Johnston, P. G.

Springer

Mammalian genome 6 (1995), S. 198-201

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ISSN: 1432-1777

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Medicine

Notes: Abstract In order to study the mechanism of X Chromosome (Chr) inactivation in marsupials, the cDNA for glucose-6-phosphate dehydrogenase (G6PD) from an Australian marsupial, the wallaroo (Macropus robustus), was cloned. A partial clone containing the 3′ half of the cDNA was obtained by screening a liver cDNA library. The majority of the coding region was obtained by polymerase chain reaction of cDNA with primers designed from regions of conservation between human and opossum G6PD. The 5′ end was obtained by rapid amplification of cDNA ends. High homology was observed between mammalian species in the coding region. The 5′ untraslated region is highly G+C rich, and appears to be part of a CpG island, as is the case in the human and mouse genes. This is the first report of the full sequence of the cDNA for any marsupial X-linked gene.