ISSN:
1432-0533
Keywords:
Recombinant retrovirus
;
Schwann cells
;
β-Galactosidase
;
Demyelination
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary This series of experiments is designed to follow the fate of implanted Schwann cells by first labeling them with a recombinant retrovirus encoding the bacterial β-galactosidase gene, then injecting them into the spinal cord after a demyclinating lesion has been produced. The label provides a means of distinguishing the exogenous Schwann cells from endogenous ones and of determining their travel pattern and myelinating or ensheathing behavior in the central nervous system (CNS). Neonatal rat primary Schwann cells were stimulated to divide by administering glial growth factor and forskolin. Fresh virus-containing supernatant from Psi2 cells producing retrovirus LZ1 was placed in cell culture to label the cells. The capacity of infected Schwann cells to form myelin was verified by coculturing in vitro with neurons from embryonic dorsal root ganglia. Infected cells were injected into the right side of adult syngenic rat spinal cords after a lysolecithin-induced demyelinating lesion had been produced 1 cm caudal on the left side. After 3 weeks the animals were killed, perfused for electron microscopy, and spinal cord sections histochemically stained for β-galactosidase activity using the chromogenic substrate 5-bromo-4-chloro-3-indoyl-β-d-galactosidase (X-Gal) which forms a blue precipitate in infected cells. The labeled cells, easily recognized macro-and microscopically, were clustered at the cell injection site, in the dorsal meninges and, at the area of demyelination, bilaterally in the superficial aspect of the dorsal funiculi. Labeled cells were not evident in the neuropil midway between the injection and demyelination sites. In the electron microscope, the cells containing the electron-dense precipitate from the hydrolysis of X-Gal were identified at the lesion site and some of these cells ensheathed axons. These data suggest that implanted Schwann cells migrate to a demyelinating lesion in the subarachnoid space rather than through the parenchyma.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00294612
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