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  • 1
    Electronic Resource
    Electronic Resource
    PO Box 1354, 9600 Garsington Road , Oxford OX4 2XG , UK . : Blackwell Science Ltd
    Geophysical prospecting 53 (2005), S. 0 
    ISSN: 1365-2478
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Geosciences , Physics
    Notes: An approach to determining the effective elastic moduli of rocks with double porosity is presented. The double-porosity medium is considered to be a heterogeneous material composed of a homogeneous matrix with primary pores and inclusions that represent secondary pores. Fluid flows in the primary-pore system and between primary and secondary pores are neglected because of the low permeability of the primary porosity. The prediction of the effective elastic moduli consists of two steps. Firstly, we calculate the effective elastic properties of the matrix with the primary small-scale pores (matrix homogenization). The porous matrix is then treated as a homogeneous isotropic host in which the large-scale secondary pores are embedded. To calculate the effective elastic moduli at each step, we use the differential effective medium (DEM) approach. The constituents of this composite medium – primary pores and secondary pores – are approximated by ellipsoidal or spheroidal inclusions with corresponding aspect ratios.We have applied this technique in order to compute the effective elastic properties for a model with randomly orientated inclusions (an isotropic medium) and aligned inclusions (a transversely isotropic medium). Using the special tensor basis, the solution of the one-particle problem with transversely isotropic host was obtained in explicit form.The direct application of the DEM method for fluid-saturated pores does not account for fluid displacement in pore systems, and corresponds to a model with isolated pores or the high-frequency range of acoustic waves. For the interconnected secondary pores, we have calculated the elastic moduli for the dry inclusions and then applied Gassmann's tensor relationships. The simulation of the effective elastic characteristic demonstrated that the fluid flow between the connected secondary pores has a significant influence only in porous rocks containing cracks (flattened ellipsoids). For pore shapes that are close to spherical, the relative difference between the elastic velocities determined by the DEM method and by the DEM method with Gassmann's corrections does not exceed 2%. Examples of the calculation of elastic moduli for water-saturated dolomite with both isolated and interconnected secondary pores are presented. The simulations were verified by comparison with published experimental data.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    British journal of dermatology 92 (1975), S. 0 
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: In order to study the biological and possible pathological roles of cyclic adenosine 3′,5′-monophsphate (cyclic AMP) in the skin, it is mandatory to measure cyclic AMP in 50–100 μg of microdissected epidermis, dermis or appendages.In the present study, we ofter a method of extracting cyclic AMP from less than 100 μg of tissue, removing contaminating nucleotides and scaling down Gilman's method to fit the analysis of small amounts of tissue. Cyclic AMP levels in the dermis, epidermis, and hair follicles (bulbs) were approximately 1, 2 and 3.5 pmols/μg dry weight tissue respectively.This procedure is applicable to the measurement of cyclic AMP levels in limited foci of healthy or diseased skin.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    British journal of dermatology 94 (1976), S. 0 
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The cyclic AMP content of epidermal slices is increased by incubation with ethanol, the effect of which is dose-dependent from 1 to 5% concentration in the incubation media.n-Propanol and acetone are also effective at a concentration equimolar to 5% ethanol.Experimental results suggest that this effect of ethanol is due to activation of adenylate cyclase, rather than to the inactivation of cyclic AMP-phosphodiesterase.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    British journal of dermatology 93 (1975), S. 0 
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: A deficiency of epidermal cyclic adenosine 3′, 5′-monophosphate (cyclic AMP) has been implicated as the cause of the incomplete differentiation, increased mitosis and increased glycogen concentration which characterize the psoriatic lesion. This communication contains our data on the epidermal concentration of cyclic AMP in the lesional and ‘normal’ (non-involved) skin of patients with psoriasis. In these studies we have introduced the following improvement in methodology: (1) Local ischaemia in the biopsy specimens was prevented by not giving any injections prior to removal of the tissues and by freezing the tissues prior to removal. (2) The epidermis was microdissected from the dermis prior to cyclic AMP assay so as to give as pure an epidermal specimen as possible.Epidermal cyclic AMP was found to be 20–25% higher in the skin lesion than in the uninvolved skin when compared on a dry weight or protein basis but essentially the same when compared on the basis of DNA content. Our conclusion is that the cyclic AMP level in the psoriatic lesion is not lower than that in the ‘normal’ skin and thus these characteristics in psoriatic lesions cannot be related simply to a cyclic AMP deficiency.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    British journal of dermatology 92 (1975), S. 0 
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: When epidermis from the uninvolved skin of psoriatic patients was incubated for 5 min in Hank's medium containing adrenaline and theophyllinc, the cychc AMP level consistently increased 20-30 times over the level observed when adrenaline was not added to the medium. On the other hand, when epidermis from the involved skin of psoriatic patients was incubated under the same experimental conditions, the cyclic AMP level increased only 2–5 times. Even when thcophylline, an inhibitor of specific cyclic AMP-phosphodiesterase, was omitted from the medium, a clearly demonstrable difference in sensitivity to adrenaline was evident in normal appearing and lesional psoriatic epidermis. These results indicate a faulty adenyl cyclase system in the involved epidermis of psoriatic lesions rather than a defective degradation process by the specific phosphodiesterase. Since the Km for adrenaline activation of adenyl cyclase was approximately the same in both the uninvolved and the involved epidermis and since the cyclic AMP increase by adrenaline was abolished by the addition of propranolol, the basic nature of the β-receptor (specifically the binding affinity to adrenaline) in the involved epidermis does not appear to be defective. On the other hand, the finding that the Value, for adrenaline activation is 10–20 times higher in the uninvoled than in the involved epidermis suggests that the poor response in the involved epidermis may be due to fewer available binding sites for adrenaline in the psoriatic lesion.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    British journal of dermatology 92 (1975), S. 0 
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The cyclic adenosine 3′,5′-monophosphate (cyclic AMP) content of pig skin was measured several seconds to minutes after removal of the skin from the body. It increased very rapidly, reached a maximum by 2 min after removal (4 times higher than the initial level), then decreased very slowly.Propranolol injected into the animal before or added after the removal of the skin did not suppress this phenomenon. The practical significance of this finding (increase of cyclic AMP level in skin after ischaemia) is obvious—in order to measure cyclic AMP level in vivo, the sample must be frozen immediately to avoid an ‘artificial’ increase in cyclic AMP.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    British journal of dermatology 107 (1982), S. 0 
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The major purpose of our studies has been to investigate various stimulators of the epidermal adenylate cyclase system. We have recognized the occurrence of four distinct adenylate cyclase systems which respond respectively to catecholamine, histamine, prostaglandin and adenosine. The exposure of floating skin slices in vitro to a stimulator causes a rapid intracellular accumulation of cyclic AMP, which is always transient. Further addition of the same stimulator will not stimulate the same receptor system against the state of ‘refractoriness’. The addition of any of the other stimulators can increase the cyclic AMP level. Furthermore, the fact that each stimulator can yield an ‘additive’ stimulatory effect leads to the conclusion that the epidermis has four distinctly specific and independent adenylate cyclase systems.Our recent investigations have been directed to the analyses of subunits of these skin surface receptor-adenylate cyclase systems. We used two experimental systems, i.e. one being a ‘leaky’ cell system in which its subunits such as receptor, GTP-regulatory protein and the catalytic unit (adenylate cyclase) are still linked togetlier, and the other being independent preparations of the receptor and catalytic units (with GTP-regulatory protein). These systems allowed us to probe the cell membrane from the inside as well as from the outside. Some of our preliminary kinetic data are herein introduced.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/General Subjects 444 (1976), S. 685-693 
    ISSN: 0304-4165
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine , Physics
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Analytica Chimica Acta 15 (1956), S. 237-245 
    ISSN: 0003-2670
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    BBA - Enzymology 429 (1976), S. 498-507 
    ISSN: 0005-2744
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
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