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  • 1
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    British journal of dermatology 80 (1968), S. 0 
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: SUMMARY.— A study of 86 infants aged 2 to 9 months who had sehorrhoeic eczema, and a similar number of clinically unaffected controls, matched for age and sex, shows that for each of 8 external areas of the skin surface the incidence of C. albicans was significantly higher among the infants with the skin disease (the differences ranged from about 8 to 39%). This conclusion was also true when only clinically affected areas of the skin surface were considered but more data are required to discover whether, as is suggested by some of the present data, a similar conclusion is justified when only clinically unaffected areas of the skin surface, of children clinically affected elsewhere, are considered.Of the infants studied, 69% of those with seborrhoeic eczema were infected with C. albicans at one or more areas of the skin surface (including the buccal mucous membrane) compared with 38% of those who had no clinical skin disease. Attention is drawn to the high incidence of C. albicans on the buccal mucous membrane of clinically unaffected infants (31·4% were so infected) and to the fact that the majority of infants infected with C. albicans had the organism either on that site, the margin of the anus, or on both. The implications of these findings for future studies of the nature of the association between C. albicans and lesions of seborrhoeic eczema are emphasized.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Oxford, UK : Blackwell Publishing Ltd
    Anaesthesia 51 (1996), S. 0 
    ISSN: 1365-2044
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    European journal of clinical microbiology & infectious diseases 9 (1990), S. 178-183 
    ISSN: 1435-4373
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract To test the value of detection of anti-Candida albicans germ tube antibodies by indirect immunofluorescence assay in the diagnosis of systemic candidosis, a retrospective study was done using 126 sera from 27 patients with presumptive systemic candidosis (13 immunocompromised), 165 sera from 45 patients with aspergillosis (29 immunocompromised), 35 sera from eight patients with cryptococcosis (6 immunocompromised), and 101 sera from 101 blood donors. While 21 of 27 patients with systemic candidosis (77.8%) had anti-germ tube antibodies, these antibodies were absent in all patients with cryptococcosis and in all blood donors. They were however detected in 5 of 45 patients with aspergillosis (11.1%). Ten of 13 (76.9%) immunocompromised patients with candidosis had anti-germ tube antibodies; similar results were obtained in immunocompetent patients with candidosis (78.6%). The specificity was 96.8%, indicating a high degree of discrimination was possible between systemic candidosis and other invasive mycoses in the patients studied. Anti-germ tube responses did not appear to be significantly reduced in immunocompromised patients.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Mycopathologia 111 (1990), S. 2-2 
    ISSN: 1573-0832
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Mycopathologia 58 (1976), S. 19-20 
    ISSN: 1573-0832
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Counterimmunoelectrophoresis (CIE) has been evaluated for routine diagnostic work, using antigens prepared from 2 different isolates of Aspergillus fumigatus, at 2 different concentrations. Additional antigens prepared in a variety of ways and 2 commercially available antigens (Bencard, London; Institut Pasteur, Paris, France) have been compared with the routine antigens. It has been shown that the use of the routine antigens will detect the majority of positive reactions. At optimal reacting concentrations antibodies were detected in 75% of asthmatic patients. In sera from patients with a presumptive diagnosis of allergic aspergillosis, CIE will detect twice as many positive reactions as a conventional agar gel diffusion test.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    European journal of epidemiology 8 (1992), S. 452-459 
    ISSN: 1573-7284
    Keywords: Yeast ; Glycoprotein secretion ; Monoclonal antibodies ; Immunodetection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Expression of antigenic epitopes shared by secreted yeast glycoproteins was studied using specific immunological probes. Application of cytological and ultrastructural methods of immunodetection, employing monoclonal antibodies, permitted us to localize these glycoproteins in the cytoplasm, through the cell wall and at the yeast cell surface. Importance of glycosylation-secretion relationships were evaluated in the secretion process of these molecules. The cell wall crossing and the cell surface distribution of antigenic glycoproteins was described in immunoelectron microscopy and immunofluorescence. Some preferential secretion “ways” were suspected through the yeast cell wall leading to an heterogenous distribution of cell surface glycoproteins destined to be excreted into the medium. Antigenic variability of cell wall glycoproteins expression was discussed in relation with the glycloprotein secretion.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    European journal of clinical microbiology & infectious diseases 9 (1990), S. 886-891 
    ISSN: 1435-4373
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A gold-silver staining procedure was evaluated for detection ofCandida species of medical importance. Probes were prepared by coupling lectins or antibodies (polyclonal and monoclonal) directly or indirectly to colloidal gold particles. Structures reacting to these probes were specifically revealed by light microscopy in cells present in infected kidney tissue sections or in isolated yeast cells on glass slides. Definition, contrast and sensitivity were of a high order. Preliminary data showed that it was possible, using discriminating dilutions, to identify cells from different species of the genusCandida, grown in vitro, according to their ability to stain with polyclonal monospecific antisera. The advantages of gold-silver staining compared with other staining procedures currently used in routine mycological laboratories are its sensitivity, good definition, ease and rapidity, and long conservation of reaction. It is suggested that the procedure has applications for research and identification of yeasts in clinical samples.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1435-4373
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract In order to study the respective roles of oligomannoside sequences in the antigenicity ofCandida albicans phosphopeptidomannan, a method was developed for constructing neoglycolipids from oligomannosides released by depolymerisation of this molecule. Oligomannosides released by acetolysis were converted to neoglycolipids by coupling them to 4-hexadecylaniline in an equimolar reaction checked by thin layer chromatography. When coated onto microEIA plates, the neoglycolipids exhibited strong reactions which were dose dependent and were saturable with concanavalin A. Reactivity of neoglycolipids with immunoglobulins were then tested with a panel of monoclonal and polyclonal antibodies reacting with epitopes present in the original phosphopeptidomannan. One of two IgM monoclonal antibodies and two of five monospecific rabbit polyclonal IgG reacted strongly with neoglycolipids therefore providing evidence of the presence of structures mimicking epitopes within the pool of neoglycolipids. When 38 sera from 18 hospital inpatients with various levels of antibodies toCandida albicans were tested, a correlation was observed between the EIA to detect neoglycolipids and the EIA to detect phosphopeptidomannan. Successive sera from all patients showing seroconversion in the immunofluorescence assay had increased EIA signals for neoglycolipids.
    Type of Medium: Electronic Resource
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