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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 90 (1986), S. 93-105 
    ISSN: 1573-5036
    Keywords: ARA ; Ecological survey ; Excised roots ; Leptochloa fusca ; MPN ; N2-fixation ; Saline soils
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary An ecological survey was made to measure the N2-fixing activity in the rhizosphere of a salt-tolerant grass,Leptochloa fusca (L.) Kunth. Samples were obtained every month at two sites over a period of one year. Soil cores, unwashed, washed and surface-sterilized roots were subjected to acetylene reduction assay (ARA). ARA values up to 50 nmoles h−1 for soil cores, 1095 nmoles g dry root−1 h−1 for unwashed roots, 4929 nmoles g dry root−1 h−1 for washed roots and 2494 nmoles g dry root−1 h−1 for surface-sterilized roots were observed but for most samples the range was 1–200 nmoles g dry root−1 h−1. A lag period of 5–7 h was observed before the onset of N2-fixing activity by excised roots and O2 levels had no effect on this lag. Values for roots incubated without preincubation were similar to those for unwashed preincubated roots. Activity was highest in September, October and November when the temperature is not very high and photosynthetic activity is reasonably good. N2-fixing-bacteria were counted on the same samples by plate count and MPN methods, the latter being estimated on the basis of ARA and pellicle formation. Fairly high numbers of bacteria (104–107) were recorded in the histoplane fraction which indicates the presence of diazotrophs in the inner cells of grass roots.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 84 (1985), S. 143-146 
    ISSN: 1573-5036
    Keywords: Acid KMnO4 extraction ; Devarda's alloy ; Mineral N ; Mineralizable N ; N availability index ; NO3 reduction ; N uptake ; Soil Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The acid permanganate method proposed as a chemical index of soil nitrogen (N) availability simply measures the mineralizable N. A modification of the method has been proposed that allows the estimation of soil mineral N simultaneously. The N values obtained by the modified method correlated highly significantly with the mineral N of incubation test (r=0.80,P〈0.01) and plant uptake (r=0.69,P〈0.01).
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 84 (1985), S. 279-282 
    ISSN: 1573-5036
    Keywords: Alkaline KMnO4 extraction ; Devarda's alloy ; Incubation ; Mineralizable N ; N availability index ; NO3-N ; N uptake ; Soil Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary To find out a rapid and reliable procedure for estimating soil available nitrogen (N), the standard alkaline-permanganate extraction and its modification to include soil NO3 were evaluated. The N values obtained with the standard method showed a poor correlation with the mineral N of incubation test and plant uptake (r=0.36 and 0.37 respectivelyP〈0.05 in both the cases) whereas the results obtained with the modified method gave highly significant correlations (r=0.84 and 0.76 respectively.P〈0.01 in both the cases). The data supplied further verification of the modified alkaline-permanganate method for assessing N availability in soils.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1573-5036
    Keywords: Fertilizer15N ; Humus components ; Immobilization-remineralization of N ; Legume residue15N ; Mineralizable N ; N balance ; N transformations ; N uptake
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The availability and turnover in different soil fractions of residual N from leguminous plant material and inorganic fertilizer was studied in a pot culture experiment using wheat as a test crop. Plants utilized 64% of the residual fertilizer N and 20% of the residual legume N. 50–60% of the N taken up by plants was recovered in grain and 4–8% in roots. After harvesting wheat up to 35% and 38% of the residual legume N and fertilizer N, respectively was found in humic compounds. A loss of humus N derived from legume and fertilizer was found during wheat growth but the unlabelled N increased in this fraction. Biomass contained 6% and 8% of the residual legume and fertilizer N, respectively when both were available. The mineralizable component contained upto 28% of both the residual legume and residual fertilizer N. Only a small percentage of the soil N (3–4%) was observed in biomass whereas the mineralizable component accounted for 7–14% of the soil N. In this fraction legume derived N increased during wheat growth whereas unlabelled N increased in both the mineralizable component and microbial biomass. Some loss of N occurred from residual legume and fertilizer N. Nevertheless, a positive total N balance was observed and was attributed to the addition of unlabelled N in the soil-plant system by N2 fixation. The gain in N was equivalent to about 38% of the plant available N in the soil amended with leguminous material. The additional N was concentrated mainly in the mineralizable fraction and microbial biomass, although some addition was also noted in humus fractions.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Mycopathologia 50 (1973), S. 339-347 
    ISSN: 1573-0832
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The object of the present investigation was to study the fungal decomposition of organic matter from different sources, added to the saline soils. The organic matter added was in the form of fresh cuttings of two salt tolerant harbaceous plants namelyDiplachne fusca (Kallar grass) andSesbania aculeata (Dhancha). In addition to these treatments, farm yard manure and press mud (waste of sugar industry) were also used as a source of organic matter. Twelve combinations of these treatments were studied. A number of fungi were isolated from differently treated saline soils incubated at 30 °C. Among the fungi isolatedAspergillus spp. andFusarium solani had the highest frequency of occurrence. The relative cellulolytic ability of 10 fungal species was also estimated.Alternaria humicola andNigrospora sp. were found to be the most cellulolytic among the fungi isolated. The decrease in organic matter due to microbial decomposition was found to be most rapid during first two weeks of incubation. Among the treatments with one organic matter source, press mud produced maximum humus equal to 0.28 %. The pH and electrical conductivity increased slightly due to the closed system where no leaching took place.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    World journal of microbiology and biotechnology 4 (1988), S. 447-453 
    ISSN: 1573-0972
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Description / Table of Contents: Résumé On a testé la faisabilité de la lixiviation bactérienne de deux minerais différents de cuivre du Pakistan au moyen de thiobacilli acidophiles,isolés localement, en culture en flaçons agités à 28 ±2°C. Après une période de latence de 16 jours,Thiobacillus thiooxidans a solubilisé jusqu'à 19% du cuivre présent dans le minerai de chalcopyrite en 14 jours avant d'entrer en phase stationnaire de croissance. Dans un minerai de cuivre amendé par le minerai de chalcopyrite et additionné de soufre de manière à fournir une source d'énergie pourT. ferrooxidans, le pH a baissé comme suite de la croissance bactérienne, ce qui a entraîné la solubilisation du cuivre. La libération la plus élevée de cuivre, 61% au total, a eu lieu dans les flaçons qui avaient à oxyder le minerai de cuivre, le soufre et la chalcopyrite oans les proportions de 1∶1∶1 en poids, et qui avaient été inoculés parT. ferrooxydans. La supplémentation effectuée dans ces tests a eu pour effet de dissoudre six fois plus de cuivre que dans les tests conduits sans supplémentation.
    Abstract: Resumen Se ha estudiado la posibilidad de realizar un lixiviado mediante bacterias de dos minerales de cobre del Pakistan. Las bacterias utilizadas son cepas, aísladas localmente, de tiobacilos acidófilos que se hacen crecer en un medio líquido en agitación a 28±2°C. Después de una fase lag de 16 dThiobacillus thiooxidans solubilizó hasta 19% del cobre presente en la calcopirita en 14 d, antes de alcanzar la fase estacionaria. El mineral de cobre oxidado se modificó con calcopirita y se suplementó con azufre afín de sumministrar una fuente de energía paraT. ferrooxidans, a resultas del crecimiento bacteriano el pH disminuyó solubilizándose cobre. La mayor cantidad de cobre sulubilizado se cencontró en frascos con mineral de cobre oxidado, azufre y calcopirita (1∶1∶1 en peso) e inoculados conT. ferrooxidans. La adición de suplementos incrementó 6 veces la solubilización comparada con la obtenida en ensayos sin adición de suplementos.
    Notes: Summary Feasibility for bacterial leaching of two different copper ores from Pakistan was investigated with locally isolated, acidophilic thiobacilli in shake flask culture at 28±2°C. After a lag period of 16 d,Thiobacillus thiooxidans solubilized up to 19% of copper present in chalcopyrite ore in 14 d before entering stationary phase. In oxidized copper ore amended with chalcopyrite ore and supplemented with sulphur to provide an energy source forT. ferrooxidans, the pH dropped as a result of bacterial growth and was accompanied by copper solubilization. The highest copper release, 61% of total, occurred in flasks having oxidized copper ore, sulphur and calcopyrite (1∶1∶1, by weight) and inoculated withT. ferrooxidans. Supplementation used in these tests resulted in nearly six times more solubilization as compared to tests conducted without supplementation.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    World journal of microbiology and biotechnology 6 (1990), S. 46-52 
    ISSN: 1573-0972
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Description / Table of Contents: Résumé It est apparu facile d'isoler des diazotrophes, principalement du genreAzospirllium à partir de racines de nombreuses plantes et utilisant un milieu malate semisolide exempt d'azote (NFM). Ces isolats forment une pellicule fine et blanche sous la surface dans le milieu malate exempt d'azote, endéans les24h. Ces pellicules ae déplacent progressivement vers la surface et exhibent des vitesses élevées de production d'acétylène. L'emploi de tests de culture sélective ou biochimique, a permis d'identifier la plupart des isolats commeAzospirillum brasilense. Quatre isolats à partir de la surface des racines d'herbe Kallar et un isolat à partir de l'intérieur d'une racine d'Atriplex forment an groupe homogène sur le plan phénotypique. Celui-ci partage de nombreuses caractéristiques avec les espèces du genreAzospirillum excepté la forme. Tous les tests biochimiques exécutés les rangent avecA. brasilense. Toutefois, leur forme ainsi que le profil protéique sur un électrophérogramme sur gel de polyacrylamide-SDS suggèrent que le groupe de cinq isolats est nettement distinct et diffère largement de toutes les souches types, appartenant à de nombreux genres.
    Notes: Abstract Diazotrophs, especially of genusAzospirillum were readily isolated from roots of many plants using semi-solid nitrogen free malate medium (NFM). These isolates formed fine, white sub-surface pellicle in nitrogen-free malate medium within 24h, which gradually moved to the surface, and exhibited high acetylene reduction rates. Using selected cultural and biochemical tests, most of the isolates were identified asAzospirillum brasilense. Four isolates from Kallar grass root surface and one isolate fromAtriplex root interior formed phenotypically a homogenous group. It shared many characteristics with the species of genusAzospirillum except shape. All the biochemical tests performed, categorized them withA. brasilense. However, the shape and the protein profile on SDS polyacrylamide gel electrophoretograms suggested that the group of these five isolates is clearly distinct and differs widely from all the type strains, belonging to various genera.
    Type of Medium: Electronic Resource
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  • 8
    ISSN: 1573-0972
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Description / Table of Contents: Résumé Une bactérie, isolée d'un échantillon d'hydrocarbures épandus, et identifiée commePseudomonas aeruginosa, dégrade l'hexaet l'heptadécane à raison respective de 47 et de 58% du carbone total avec 9 et 12% des substrats respectifs libérés sous la forme de CO2. Avec l'octa- et le nonadécane comme substrats, la biodégradation atteint respectivement 73 et 60% avec 27 et 25% du carbone total transformé en CO2. On a étudié la production de biosurfactant par cette bactérie en utilisant l'hexadécane à 5% (v/v) comme substrat. La tension superficielle de la liqueur mixte, comme celle de son surnageant atteint 30 mN/m par comparaison à 71 mN/m pour l'eau. Lorsque le surnageant est mélangé à l'hexadécane (1∶5, v/v), on forme une émulsion stable qui ne se détériore que de 10% au bout d'un mois.
    Notes: Summary A bacterium isolated from an oil spillage sample and identified asPseudomonas aeruginosa degraded hexadecane and heptadecane by 47% and 58% with 9% and 12% of total carbon from the respective substrates being liberated as CO2. With octadecane and nonadecane as substrates, 73% and 60% were biodegraded while 27% and 25% of total carbon was evolved as CO2, respectively. Production of biosurfactant by this bacterium was studied using hexadecane 5% (v/v) as substrate. The surface tension of spent culture medium, as well as the supernatant, was 30 mN/m compared to 71 mN/m for water. When the supernatant was mixed with hexadecane (1∶5, v/v) a stable emulsion was formed which deteriorated only by 10% after one month.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Plant cell, tissue and organ culture 51 (1997), S. 201-207 
    ISSN: 1573-5044
    Keywords: Gossypium hirsutum L. ; leaf curl virus ; meristem tip culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In order to develop transgenic plants via the biolistic gun method regenerable embryogenic tissues are required. Meristem shoot tips of 19 cultivars of cotton were cultured on several media formulations and assessed for shoot and root development. The best shoot development was observed on media containing 0.46 mM kinetin while rooting was observed on media containing 2.68 mM NAA and 0.46 mM kinetin. No intervarietal variability was observed. A complete protocol was developed from meristem tip culture to field transfer. This methodology is simple and replaces the existing protocols for meristem tip culture of cotton.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1573-5036
    Keywords: inoculation ; N2-fixation ; salinity ; Vigna radiata
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract This study reports the effect of salinity and inoculation on growth, ion uptake and nitrogen fixation byVigna radiata. A soil ECe level of 7.5 dS m−1 was quite detrimental causing about 60% decline in dry matter and grain yield of mungbean plants whereas a soil ECe level of 10.0 dS m−1 was almost toxic. In contrast most of the studied strains of Rhizobium were salt tolerant. Nevertheless, nodulation, nitrogen fixation and total nitrogen concentration in the plant was drastically affected at high salt concentration. A noticeable decline in acetylene reduction activity occurred when salinity level increased to 7.5 dS m−1.
    Type of Medium: Electronic Resource
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