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  • 1
    Electronic Resource
    Electronic Resource
    Effects of picric acid-formaldehyde fixation on the LH(HCG) receptors' binding activity in the rat testis (1977)
    Springer
    Histochemistry and cell biology 52 (1977), S. 129-143 
    Details
    ISSN: 1432-119X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary PAF (picric acid-formaldehyde) fixation of rat testis for a short time at 0–4°C was found to give satisfactory histological results and to preserve most of the specific binding activity of LH(HCG) receptors. Investigations of the characteristics of the hormone-receptor reaction after mild PAF fixation indicated that this reaction was not substantially affected in hormone-receptor affinity and in its own specificity; only the capacity of the receptors was lowered by about 20%. A histochemical model is presented whose main features are: fixation of testis tissue in PAF; freezing in liquid nitrogen and cutting in the cryostat; radiolabelled hormone-receptor binding reaction performed on the sections; autoradiography to reveal the binding reaction. The utility of the method for qualitative and quantitative receptor studies and its possible application to biopsy and surgical specimens, are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00492289
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Autoradiographic study of the distribution of LH(HCG) receptors in the ovary of untreated and gonadotrophin-primed immature rats (1977)
    Springer
    Cell & tissue research 183 (1977), S. 329-342 
    Details
    ISSN: 1432-0878
    Keywords: Ovary (Rat) ; Luteinization ; LH(HCG) Receptors ; Autoradiography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The LH(HCG) receptors in the ovaries of immature rats which were either untreated, or primed with PMSG and HCG, have been studied with a histochemical method which has proved to be as effective as when earlier used in the rat testis. This method, which consists of the topical application of 125I-HCG to picric acid-formaldehyde (PAF) fixed frozen sections followed by autoradiography, is also suitable for quantitative studies on the distribution of receptors. In the ovary of the immature 26 days old rat, the LH(HCG) receptors are localized exclusively in the interstitial and thecal tissues. After PMSG treatment many receptors appear in the granulosa of the large antral follicles. These receptors are most numerous in the outer layers of cells and least numerous in the inner. At the same time there are fewer receptors in the thecal and interstitial cells which have undergone the process of luteinization. After PMSG and HCG treatment the newly formed corpora lutea have few receptors, but these become progressively more numerous on subsequent days. It is suggested that, in the rat, the luteinization of the ovarian LH-target cells is associated with an initial decrease in the number of their LH(HCG) receptors.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00220640
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    A histochemical study of the binding of 125I-HCG to the rat ovary throughout the estrous cycle (1979)
    Springer
    Cell & tissue research 197 (1979), S. 213-226 
    Details
    ISSN: 1432-0878
    Keywords: Ovary (Rat) ; Estrous cycle ; 125I-HCG Uptake “in vitro” Autoradiography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Changes in the distribution of the in vitro uptake of 125I-HCG by the ovaries of adult rats were examined histochemically throughout the estrous cycle. Only in follicles wider than 500 μm, occurring mainly at diestrus and proestrus, could granulosa cells bind the labelled hormone. The labelling increased with follicular size and decreased in intensity from the peripheral granulosa cells inwards. No uptake occurred in the oocytes, in the cells of the cumulus oophorus nor in the granulosa cells of the atretic follicles. The binding capacity of the newly-formed corpora lutea of estrus was less than that of preovulatory follicles. The uptake of 125I-HCG by corpora lutea during the first cycle reached its maximum at diestrus but fell sharply by proestrus. The uptake was patchy in the corpora lutea of the second cycle and not significant in the older ones. The uptake of 125I-HCG by thecae increased with follicular size and was greater in the thecae of atretic follicles than in the thecae of growing follicles of like size. There was a greater uptake in the last formed interstitial tissue than there was in older tissue. At proestrus, the uptake of 125I-HCG was unaffected by the LH surge at 18.00h but had decreased slightly at 24.00 h. The implications of these data in relation to the regulation of receptor sites, is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00233915
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  • 4
    Electronic Resource
    Electronic Resource
    In vitro binding of 125I-HCG to the rat ovary from birth to puberty (1981)
    Springer
    Cell & tissue research 214 (1981), S. 633-640 
    Details
    ISSN: 1432-0878
    Keywords: Rat ovary ; 125I-HCG binding ; Autoradiography ; Hormone receptors
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Neither homogenates nor frozen ovarian sections of rats aged 1, 5 and 7 days demonstrated specific binding of 125I-HCG. However, from day 10 to day 35, the homogenates could bind specifically, with an equilibrium association constant in the range of 0.9–2.7 × 1010 M−1. The binding capacity increased from 5.8 fmol/mg tissue at day 10 to 15.7 fmol/mg tissue at day 35. As revealed by autoradiography, the frozen ovarian sections from 10-day-old rats showed 125I-HCG binding localized over the interstitial and thecal tissues and, in the older age groups, also in the granulosa cells of follicles larger than 500 μm in diameter. These results indicate that LH/HCG receptors appear in the rat ovary at the beginning of the second postnatal week, and that the interstitial cells are the main site of action of LH before puberty.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00233502
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    Paper (German National Licenses)
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