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  • 1
    Electronic Resource
    Electronic Resource
    Fine structure of the testicular gland of the black goby, Gobius jozo L. (1974)
    Springer
    Cell & tissue research 154 (1974), S. 39-49 
    Details
    ISSN: 1432-0878
    Keywords: Gobius jozo (Teleost) ; Testis ; Steroidogenic tissue ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The homologue of mammalian Leydig cells in the testis of fish of the genus Gobius is believed to be a glandular mass lying along the mesorchium and quite distinct from the seminiferous region. This gland was studied in Gobius jozo by light and electron microscopy. Histologically, the gland is composed of cords of polyhedral cells located between the main vessels of the testis and the deferent duct. Dark and light cell varieties were observed both after staining with toluidine blue and with respect to their cytoplasmic electron density. The fine structure of the glandular cells is characterized by a well developed agranular endoplasmic reticulum in the form of random anastomosing tubules and vesicles and by very numerous mitochondria with tubulo-vesicular cristae. In some mitochondria the internal organization is paracrystalline. One or two bodies of unknown nature are Found in the Golgi region. They are partially limited by an unusually thick membrane and contain a material subdivided mostly into small vesicles. Liposomes and lipofuscin pigment granules are also present. The ultrastructural features of the glandular cells of the testis of Gobius jozo confirm a specialization in steroid hormone production.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00221070
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    In vitro binding of 125I-HCG to the rat ovary from birth to puberty (1981)
    Springer
    Cell & tissue research 214 (1981), S. 633-640 
    Details
    ISSN: 1432-0878
    Keywords: Rat ovary ; 125I-HCG binding ; Autoradiography ; Hormone receptors
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Neither homogenates nor frozen ovarian sections of rats aged 1, 5 and 7 days demonstrated specific binding of 125I-HCG. However, from day 10 to day 35, the homogenates could bind specifically, with an equilibrium association constant in the range of 0.9–2.7 × 1010 M−1. The binding capacity increased from 5.8 fmol/mg tissue at day 10 to 15.7 fmol/mg tissue at day 35. As revealed by autoradiography, the frozen ovarian sections from 10-day-old rats showed 125I-HCG binding localized over the interstitial and thecal tissues and, in the older age groups, also in the granulosa cells of follicles larger than 500 μm in diameter. These results indicate that LH/HCG receptors appear in the rat ovary at the beginning of the second postnatal week, and that the interstitial cells are the main site of action of LH before puberty.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00233502
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Sertoli and Leydig cell numbers and gonadotropin receptors in rat testis from birth to puberty (1990)
    Springer
    Cell & tissue research 260 (1990), S. 185-191 
    Details
    ISSN: 1432-0878
    Keywords: Testicular development ; Sertoli cells ; Leydig cells ; Gonadotropin receptors ; Rat (Wistar)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In testes of rats from 2 to 60 days of age, we examined the number of Sertoli cells (SC) and Leydig cells (LC) as well as the binding of radioiodinated gonadotropins to frozen sections and homogenates. The number of SC per testis increased only during the first 2 postnatal weeks, whereas that of LC was stable up to days 7–10 and increased thereafter. The uptake of 125I-labelled human follicle-stimulating hormone (125I-FSH) to frozen sections was confined to sex cords or seminiferous tubules, while that of 125I-labelled human choriogonadotropin (125I-hCG) matched the distribution of LC in the interstitium. High affinity receptors for FSH and hCG were found in homogenates at all stages studied. The number of FSH receptors per testis increased steadily, whereas that of hCG receptors was low until days 7–10 and rose afterwards. Thus, SC in rat testis appear to proliferate in the presence of fetal LC during the first 2 postnatal weeks and to differentiate concomitantly with the emergence of the adult LC generation after day 10. The complement of FSH receptors in SC remains constant as they proliferate and increases after day 21 as they differentiate. The hCG receptor number is relatively fixed in each LC generation, being higher in adult compared to fetal LC.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00297504
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    Paper (German National Licenses)
    Fulltext
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