ISSN:
0173-0835
Keywords:
Chemistry
;
Biochemistry and Biotechnology
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
The resolution of angiotensinogen (Mr56 800) linked to a decapeptide, angiotensin I (Mr 1295), from the same protein without the decapeptide presents a challenging separation problem. Polyacrylamide gel electrophoresis at an optimized pH of 6.88 is unable to distinguish between the two proteins with statistical significance on the basis of size, but is able to discriminate between them with significance on the basis of their net charge difference. Correspondingly, the proteins are separated in gel electrofocusing with a pI difference of about 0.1 pH unit. For practical purposes, neither the charge separation by gel electrophoresis nor that by steady-state electrofocusing seems sufficiently convenient. By contrast, transient state electrofocusing provides a ready means for their separation.
Additional Material:
4 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/elps.1150060503
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