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  • 1
    Electronic Resource
    Electronic Resource
    Severe IgE-mediated anaphylaxis following consumption of fried frog legs: definition of α-parvalbumin as the allergen in cause (2002)
    Oxford, UK : Munksgaard International Publishers
    Allergy 57 (2002), S. 0 
    Details
    ISSN: 1398-9995
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Background: IgE-mediated allergic reactions to bullfrog and edible frog have been reported. The implicated allergens have not been defined so far. The frog material and the patient's serum from a case of severe food-induced anaphylaxis were used to define the implicated allergen at the protein and DNA level.Methods: Immunoblotting techniques and N-terminal protein microsequencing were used to define the allergen recognized by the patient's serum. Back translation from the identified protein sequence was used to design degenerated primers to amplify the allergen's cDNA by polymerase chain reaction (PCR). We defined the nucleotide sequence of the allergen from the frog of Indonesian origin that was consumed by the patient, and the homologous cDNA from Rana esculenta.Results: Protein microsequencing revealed that the implicated frog allergen belonged to the parvalbumin family. cDNAs coding for α- and β-parvalbumin of R. esculenta and Rana species were cloned. Recombinant proteins were expressed in Escherichia coli. The patient's serum IgE antibodies recognized parvalbumin prepared from frog muscle and recombinant α-parvalbumin from R. species but not from R. esculenta. Recombinant β-parvalbumin was not recognized by the IgE antibodies.Conclusion: This work defines at the protein and DNA levels α-parvalbumin as the allergen implicated in a case of IgE-mediated anaphylaxis to frog muscle. It also shows that a protein belonging to the parvalbumin family is implicated in type I allergies outside the fish species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1034/j.1398-9995.2002.23677.x
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  • 2
    Electronic Resource
    Electronic Resource
    Assessment of accuracy of renal artery stenosis grading in helical CT angiography using maximum intensity projections (1996)
    Springer
    European radiology 6 (1996), S. 658-664 
    Details
    ISSN: 1432-1084
    Keywords: Helical CT ; Blood vessels, CT ; Renal arteries, stenosis ; Renal arteries, CT
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The purpose of the study was to investigate whether visual inspection of maximum intensity projection (MIP) images is a reliable method for assessment of the severity of renal artery stenoses (RAS). Therefore, 20 RAS were investigated with helical CT. Native axial images and MIP images were analysed separately or in combination (“two-step method”). A phanton study was performed to investigate the influence of window setting on apparent stenosis severity. Accuracy for diagnosis of 50–69% and 70–99% RAS and the mean inter-observer agreement were 82.5%, 77.5% and 82.5% respectively, using study of MIP images alone, and 100%, 97.5% and 95% using the “two-step method”. The phantom study showed that the apparent severity of vascular stenoses on MIP images depends on the selected window centre values. It is concluded that combined evaluation of both MIP images and native axial images is more reliable than study of MIP images alone for assessing the severity of RAS.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00187668
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    Paper (German National Licenses)
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