ISSN:
1439-0523
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
Notes:
The effect of ethylene on gene transfer mediated by an Agrobacterium tumefaciens harbouring a binary vector with the β-glucuronidase (uid A) gene was investigated in melon, Cucumis melo L. Explants excised from melon cotyledons produced ethylene, the production of which was increased by the addition of 1-aminocyclopropane-1-carboxylic acid (ACC, 20 or 200 μM), and inhibited by the addition of aminoethoxy-vinylglycin (AVG, 10 μM). Agrobacterium inoculation of explants increased ethylene production, while application of AVG during inoculation reduced it. After 4 days of co-cultivation with Agrobacterium, gene transfer in the explants was assayed by transient uid A expression. Application of ACC to the co-cultivation medium reduced Agrobacterium-mediated gene transfer to explants and that of AVG increased it. These results suggest that ethylene affects the A. tumefaciens-mediated gene transfer to the explants excised from melon cotyledons, and the efficiency of Agrobacterium-mediated gene transfer can be improved by inhibiting ethylene production from the explants.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1046/j.1439-0523.2000.00438.x
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