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  • 1
    Electronic Resource
    Electronic Resource
    Properties of the three cell types in sixteen-cell sea urchin embryos: RNA synthesis
    Amsterdam : Elsevier
    Developmental Biology 27 (1972), S. 457-478 
    Details
    ISSN: 0012-1606
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0012-1606(72)90186-8
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Gap junction proteins exhibit early and specific expression during intramembranous bone formation in the developing chick mandible (1994)
    Springer
    Anatomy and embryology 190 (1994), S. 231-241 
    Details
    ISSN: 1432-0568
    Keywords: Connexin42 ; Connexin43 ; Connexin45 ; Chick embryo ; Osteogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The spatial and temporal expression of three closely related members of the connexin family of gap junction proteins (connexin42, Cx42; connexin43, Cx43; and connexin45, Cx45) was evaluated during bone formation in the mandibular process of the chick embryo. Mandibles of chick embryos from Hamburger and Hamilton stage 25 (approximately 5 days) through 19 days of development were dissected, serially sectioned and processed for immunocytochemical localization, employing site-specific anti-connexin antibodies. Our data revealed that (1) Cx43 was present throughout mandibular bone formation; (2) although it appeared to be associated with all bone cell types, Cx43 was concentrated in mesenchymal cells during the earliest stages in the osteogenic lineage; (3) most importantly, the localization of Cx43 at sites of bone formation appeared to precede the overt expression of the osteogenic phenotype; (4) by contrast, Cx45 was more restricted, spatially and temporally, in its distribution; (5) Cx42 expression was not detected in osteogenic tissue during mandibular bone formation. From all of the data obtained, Cx45 appeared to be associated with stages of bone formation characterized by the elaboration of matrix and the progressive expression of the differentiated osteogenic phenotype. Cx43 appeared to be associated with condensation of mesenchyme and the earliest stages of osteogenesis. Because of these associations, we propose that connexin expression may be necessary for the initiation of bone formation and the full expression of the osteogenic phenotype.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00234301
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Immunolocalization of connexin 43 in the tooth germ of the neonatal rat (1994)
    Springer
    Journal of molecular histology 26 (1994), S. 765-770 
    Details
    ISSN: 1573-6865
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Rabbit polyclonal antibodies to amino acids 346–360 of connexin 43, the ‘heart’ gap junction protein, were employed to immunolocalize connexin 43 gap junctions in the neonatal rat molar tooth germ. Connexin 43 appears early in the differentiation of both ectodermally derived and ectomesenchymally derived cells of the developing tooth. Connexin 43 immunoreactivity is present in the epithelial components of the enamel organ, including the area of the proximal and distal junctional complexes of the ameloblast layer, and the stratum intermedium, stellate reticulum and outer enamel epithelium. Secretory odontoblasts and developing alveolar bone also display a pattern of connexin 43 immunostaining. Both the epithelial and ectomesenchymally-derived components of the developing tooth acquire connexin 43 channels in a manner that correlates with cell differentiation. In addition, three regions can be defined by connexin 43 immunostaining: the epithelia of the enamel organ that are derived from the oral epithelium, the odontoblast layer derived from the ectomesenchyme, and the alveolar bone. The results suggest that connexin 43 may provide the mechanism for functional compartmentalization of the tissues associated with tooth formation. Compartmentalization suggested by connexin 43 expression could play important roles in the development and functions of these tissues.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00188074
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Immunolocalization of connexin 43 in the tooth germ of the neonatal rat (1994)
    Springer
    Journal of molecular histology 26 (1994), S. 765-770 
    Details
    ISSN: 1573-6865
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Rabbit polyclonal antibodies to amino acids 346–360 of connexin 43, the ‘heart’ gap junction protein, were employed to immunolocalize connexin 43 gap junctions in the neonatal rat molar tooth germ. Connexin 43 appears early in the differentiation of both ectodermally derived and ectomesenchymally derived cells of the developing tooth. Connexin 43 immunoreactivity is present in the epithelial components of the enamel organ, including the area of the proximal and distal junctional complexes of the ameloblast layer, and the stratum intermedium, stellate reticulum and outer enamel epithelium. Secretory odontoblasts and developing alveolar bone also display a pattern of connexin 43 immunostaining. Both the epithelial and ectomesenchymally-derived components of the developing tooth acquire connexin 43 channels in a manner that correlates with cell differentiation. In addition, three regions can be defined by connexin 43 immunostaining: the epithelia of the enamel organ that are derived from the oral epithelium, the odontoblast layer derived from the ectomesenchyme, and the alveolar bone. The results suggest that connexin 43 may provide the mechanism for functional compartmentalization of the tissues associated with tooth formation. Compartmentalization suggested by connexin 43 expression could play important roles in the development and functions of these tissues.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02388633
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    An implant labeling technique employing sable hair probes as carriers for 3H-thymidine: Applications to the study of facial morphogenesis (1984)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 210 (1984), S. 525-536 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: An implant labeling technique is described that utilizes sable hair probes as carriers for a tritiated thymidine marker. The protocol that was developed produced localized labeling of specific embryonic cell populations. This procedure was applied to the analysis of facial process development in chick embryos.Evaluation of the technique demonstrated that the probe preparation procedure was consistently successful in producing labeled probes. Using labeled probes, the procedure was reliable in producing acceptable levels of labeling in chick embryonic tissues and labeling of localized cell populations was possible using the implant labeling technique. Surrounding the center of labeling, a gradient in intensity of labeling was often observed. This pattern presumably reflects declining availability of labeled thymidine as distance from the probe increased. The technique allowed excellent survival rates to be achieved provided that aseptic procedures were followed. Additionally, careful analysis of older embryos failed to reveal any malformations induced by the implant labeling procedure.The localized labeling patterns that were demonstrated during this investigation suggest that the implant labeling technique would provide a useful tool for following cell migration during facial process formation.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1092100313
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    Paper (German National Licenses)
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