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  • 1
    Electronic Resource
    Electronic Resource
    Oil bodies and their associated proteins, oleosin and caleosin (2001)
    Copenhagen : Munksgaard International Publishers
    Physiologia plantarum 112 (2001), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Oil bodies are lipid storage organelles which have been analyzed biochemically due to the economic importance of oil seeds. Although oil bodies are structurally simple, the mechanisms involved in their formation and degradation remain controversial. At present, only two proteins associated with oil bodies have been described, oleosin and caleosin. Oleosin is thought to be important for oil body stabilization in the cytosol, although neither the structure nor the function of oleosin has been fully elucidated. Even less is known about caleosin, which has only recently been described [Chen et al. (1999) Plant Cell Physiol 40: 1079–1086; Næsted et al. (2000) Plant Mol Biol 44: 463–476]. Caleosin and caleosin-like proteins are not unique to oil bodies and are associated with an endoplasmatic reticulum subdomain in some cell types. Here we review the synthesis and degradation of oil bodies as they relate to structural and functional aspects of oleosin and caleosin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1034/j.1399-3054.2001.1120301.x
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  • 2
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    "Ketzer und Professoren: Häresie und Vernunftglaube im Hochmittelalter". (Heinrich Fichtenau) (Book Review) (1993)
    Washington : Periodicals Archive Online (PAO)
    Catholic historical review. 79:3 (1993:July) 518 
    Details
    ISSN: 0008-8080
    Topics: History , Theology and Religious Studies
    Description / Table of Contents: Ancient and Medieval
    Notes: BOOK REVIEWS
    URL: http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&res_dat=xri:pao:&rft_dat=xri:pao:article:1053-1993-079-03-000020
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  • 3
    Electronic Resource
    Electronic Resource
    Gut protection by cyclophosphamide “priming” in patients receiving high-dose melphalan — effect of drug scheduling (1992)
    Springer
    Cancer chemotherapy and pharmacology 30 (1992), S. 149-151 
    Details
    ISSN: 1432-0843
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A “priming” injection of cyclophosphamide (400 mg/m2 given i.v. on day −7) has been shown to reduce intestinal permeability and thus gut toxicity in patients receiving high-dose melphalan. To determine the optimal timing for this injection patients receiving 200 mg/m2 melphalan with an autologous bone marrow transplant were randomly assigned to receive cyclophosphamide at 5, 7 or 9 days before the melphalan. The median percentage of [51Cr]-ethylenediaminetetraacetic acid excretion was similar (9.1% vs 7.1% vs 7.7%, respectively), with equivalent duration of WHO grade 2–4 mucositis and diarrhoea being recorded for each group. Thus, the timing of the cyclophosphamide prime is not critical, and the priming injection may be given between 5 and 9 days prior to high-dose melphalan.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00686408
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  • 4
    Electronic Resource
    Electronic Resource
    Preparation of Pooled Arabidopsis YAC DNAs for PCR-Based Mapping (1999)
    Springer
    Plant molecular biology reporter 17 (1999), S. 67-71 
    Details
    ISSN: 1572-9818
    Keywords: physical mapping ; yeast DNA purification and pooling
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Physical mapping of an arabidopsis DNA sequence of interest can easily be performed by PCR. This is done by using specific primers and pooled DNA templates isolated from publicly available YAC or BAC libraries. We present simple protocols for preparing pooled YAC DNAs and PCR-based detection of sequences in them by which several sequences can be mapped in a short time.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1007564130408
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  • 5
    Electronic Resource
    Electronic Resource
    Analysis of an ABA-responsive rice gene promoter in transgenic tobacco (1990)
    Springer
    Plant molecular biology 15 (1990), S. 905-912 
    Details
    ISSN: 1573-5028
    Keywords: rice ; developmental regulation ; embryo-specific
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have analyzed in transgenic tobacco the expression of a chimeric gene containing 5′ sequences of the rice rab-16B gene fused to the β-glucuronidase (GUS) reporter gene. This construct, a translational fusion (−482 to +184) including 14 amino acids of the RAB-16B protein, is expressed only in zygotic and pollen-derived embryos. In zygotic embryos, GUS activity begins to accumulate 10 days after flowering (daf), and increases until seed maturation at 25 daf. Immunological measurements of endogenous abscisic acid (ABA) accumulation in these seeds showed a close parallel between hormone levels and GUS activity. However, GUS activity could not be reproducibly induced by treatment of immature embryos with ABA (10 μM). Neither GUS activity nor GUS mRNA could be detected in leaves of transgenic tobacco even after ABA treatment. In contrast, GUS activity could be induced to high levels in pollen-derived embryos by treatment with ABA. Our results show that 482 bp of 5′ sequences of the rice rab-16B promoter can confer in transgenic tobacco developmentally regulated expression in embryos but not ABA-responsive expression in vegetative tissues.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00039429
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  • 6
    Electronic Resource
    Electronic Resource
    Arabidopsis ATP A2 peroxidase. Expression and high-resolution structure of a plant peroxidase with implications for lignification (2000)
    Springer
    Plant molecular biology 44 (2000), S. 231-243 
    Details
    ISSN: 1573-5028
    Keywords: Arabidopsis thaliana ; lignification ; monolignols ; plant peroxidase ; promoter ; X-ray structure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Lignins are phenolic biopolymers synthesized by terrestrial, vascular plants for mechanical support and in response to pathogen attack. Peroxidases have been proposed to catalyse the dehydrogenative polymerization of monolignols into lignins, although no specific isoenzyme has been shown to be involved in lignin biosynthesis. Recently we isolated an extracellular anionic peroxidase, ATP A2, from rapidly lignifying Arabidopsis cell suspension culture and cloned its cDNA. Here we show that the Atp A2 promoter directs GUS reporter gene expression in lignified tissues of transgenic plants. Moreover, an Arabidopsis mutant with increased lignin levels compared to wild type shows increased levels of ATP A2 mRNA and of a mRNA encoding an enzyme upstream in the lignin biosynthetic pathway. The substrate specificity of ATP A2 was analysed by X-ray crystallography and docking of lignin precursors. The structure of ATP A2 was solved to 1.45 Å resolution at 100 K. Docking of p-coumaryl, coniferyl and sinapyl alcohol in the substrate binding site of ATP A2 were analysed on the basis of the crystal structure of a horseradish peroxidase C-CN-ferulic acid complex. The analysis indicates that the precursors p-coumaryl and coniferyl alcohols are preferred by ATP A2, while the oxidation of sinapyl alcohol will be sterically hindered in ATP A2 as well as in all other plant peroxidases due to an overlap with the conserved Pro-139. We suggest ATP A2 is involved in a complex regulation of the covalent cross-linking in the plant cell wall.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006442618860
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  • 7
    Electronic Resource
    Electronic Resource
    Common amino acid sequence domains among the LEA proteins of higher plants (1989)
    Springer
    Plant molecular biology 12 (1989), S. 475-486 
    Details
    ISSN: 1573-5028
    Keywords: LEA protein sequence ; plant desiccation ; abscisic acid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract LEA proteins are late embryogenesis abundant in the seeds of many higher plants and are probably universal in occurrence in plant seeds. LEA mRNAs and proteins can be induced to appear at other stages in the plant's life by desiccation stress and/or treatment with the plant hormone abscisic acid (ABA). A role in protecting plant structures during water loss is likely for these proteins, with ABA functioning in the stress transduction process. Presented here are conserved tracts of amino acid sequence among LEA proteins from several species that may represent domains functionally important in desiccation protection. Curiously, an 11 amino acid sequence motif is found tandemly repeated in a group of LEA proteins of vastly different sizes. Analysis of this motif suggests that it exists as an amphiphilic α helix which may serve as the basis for higher order structure.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00036962
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  • 8
    Electronic Resource
    Electronic Resource
    Differential expression of two related organ-specific genes in pea (1990)
    Springer
    Plant molecular biology 14 (1990), S. 765-774 
    Details
    ISSN: 1573-5028
    Keywords: pea ; tissue-specific gene expression ; stem ; pod
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have screened a pea genomic library using a cDNA probe derived from pea shoot RNA. From this screen, we isolated two closely related genes, designated as S2 and P4. An intriguing property of these two genes is the presence in their coding region of a repeated sequence that is conserved between them in sequence but not in the number of the repeating units. The predicted amino acid sequence suggests that these proteins could be exported and glycosylated. 3′ S1 analysis reveals that one of the genes, S2, is expressed highly in stem, as expected from previous work. However, mRNA derived from the other gene, P4, is not detectable in stem tissue, but is present in tissue derived from pea pods. The 5′ upstream sequence of S2 and P4 are 94% identical up to position -121, suggesting that sequences upstream of -121 are responsible for organ-specific expression of the two genes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00016509
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  • 9
    Electronic Resource
    Electronic Resource
    Structure and expression of the barley lipid transfer protein gene Ltp1 (1992)
    Springer
    Plant molecular biology 18 (1992), S. 585-589 
    Details
    ISSN: 1573-5028
    Keywords: aleurone layer ; enzyme inhibitor ; gene sequence ; transient expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have characterized a gene (Ltp1) encoding a barley lipid transfer protein. Northern blot analysis showed that Ltp1 mRNA accumulates specifically in the aleurone layer of developing and germinating seeds. Southern blot analysis indicated that LTP1 protein is encoded by a single gene in barley. Sequence analysis of Ltp1 showed that it contains an open reading frame of 351 bp interrupted by a single intron of 133 bp. Transient expression assays indicated that 702 bp of the 5′ upstream region of Ltp1 is sufficient to direct aleurone-specific expression during late seed development and early germination.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00040674
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  • 10
    Electronic Resource
    Electronic Resource
    Regulation of the maizerab17 gene promoter in transgenic heterologous systems (1991)
    Springer
    Plant molecular biology 17 (1991), S. 985-993 
    Details
    ISSN: 1573-5028
    Keywords: rab17 promoter ; abscisic acid ; transgene expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The maizerab17 gene is expressed in different plant parts in response to ABA and osmotic stress (J. Vilardellet al., Plant Mol Biol 14 (1990) 423–432). Here we demonstrate that 5′ upstream sequences of therab17 gene confer the appropriate patterns of expression on the chloramphenicol acetyl transferase (CAT) reporter gene in transgenic tobacco plants, as well as in protoplasts derived from cultured rice cells. Specifically, a CAT construct containing a large 5′ upstream fragment ofrab17 (−1330/+29) results in high levels of CAT activity in embryos, leaves and roots of transgenic plants subjected to water stress or ABA treatment. Transient expression assays in rice protoplasts transfected with CAT genes fused torab17 promoter deletions indicate that a 300 bp DNA fragment (−351/−102) is sufficient to confer ABA responsiveness upon the reporter gene. Furthermore, a 100 bp sequence (−219/−102) is capable of conferring ABA responsiveness upon a minimal promoter derived from the 35S CaMV promoter. Gel retardation experiments indicate that maize nuclear proteins bind to this fragment. This region of 100 bp contains a sequence (ACGTGGC) which has been identified as an abscisic acid response element in studies of other ABA-responsive plant genes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00037138
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