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  • 1
    Electronic Resource
    Electronic Resource
    Powering a two-stroke RNA engine (2007)
    [s.l.] : Nature Publishing Group
    Nature structural & molecular biology 14 (2007), S. 574-576 
    Details
    ISSN: 1545-9985
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Medicine
    Notes: [Auszug] The proposal that primordial life originated in an RNA world is based upon the dual capacity of RNA molecules to hold genetic information and catalyze chemical reactions. Peptide bond formation within the ribosome may be a 'living fossil' of the ancient catalytic properties of RNA. Available ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nsmb0707-574
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  • 2
    Electronic Resource
    Electronic Resource
    The cis-regulatory element CCACGTGG is involved in ABA and water-stress responses of the maize gene rab28 (1993)
    Springer
    Plant molecular biology 21 (1993), S. 259-266 
    Details
    ISSN: 1573-5028
    Keywords: ABA-responsive element ; maize ; tissue-specific factors ; rab genes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The maize gene rab28 has been identified as ABA-inducible in embryos and vegetative tissues. It is also induced by water stress in young leaves. The proximal promoter region contains the conserved cis-acting element CCACGTGG (ABRE) reported for ABA induction in other plant genes. Transient expression assays in rice protoplasts indicate that a 134 bp fragment (-194 to -60 containing the ABRE) fused to a truncated cauliflower mosaic virus promoter (35S) is sufficient to confer ABA-responsiveness upon the GUS reporter gene. Gel retardation experiments indicate that nuclear proteins from tissues in which the rab28 gene is expressed can interact specifically with this 134 bp DNA fragment. Nuclear protein extracts from embryo and water-stressed leaves generate specific complexes of different electrophoretic mobility which are stable in the presence of detergent and high salt. However, by DMS footprinting the same guanine-specific contacts with the ABRE in both the embryo and leaf binding activities were detected. These results indicate that the rab28 promoter sequence CCACGTGG is a functional ABA-responsive element, and suggest that distinct regulatory factors with apparent similar affinity for the ABRE sequence may be involved in the hormone action during embryo development and in vegetative tissues subjected to osmotic stress.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00019942
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  • 3
    Electronic Resource
    Electronic Resource
    Regulation of the rab17 gene promoter in transgenic Arabidopsis wild-type, ABA-deficient and ABA-insensitive mutants (1994)
    Springer
    Plant molecular biology 24 (1994), S. 561-569 
    Details
    ISSN: 1573-5028
    Keywords: Arabidopsis ; ABA mutants ; ABA-responsive genes ; promoter ; transgenic plants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The abscisic acid-responsive gene rab17 is induced during maize embryo maturation and in vegetative tissues under water stress conditions. To investigate how ABA is involved in the induction of the rab17 gene, we present here a genetic approach to analyse the transcriptional regulation of the 1.3 kb rab17 promoter fragment in transgenic wild-type Arabidopsis and mutants which are deficient (aba) and insensitive (abi1, abi2 and abi3) to ABA. During seed development the rab17 promoter fragment confers similar temporal and spatial regulation on the reporter gene GUS, both in transgenic wild-type and ABA-deficient and ABA-insensitive mutants. The rab17 promoter was only active in embryo and endosperm during late seed development, although the ABA-deficient embryo mutant showed a reduction in the level of GUS activity. During germination rab17 promoter activity decreases, and GUS activity is not enhanced by water stress in transgenic wild-type and mutant plants. In contrast, transcription of the Arabidopsis endogenous rab gene is stimulated by water stress, both in wild-type and ABA-insensitive mutants. Our data suggest that different molecular mechanisms mediate seed-specific expression and ABA water stress induction of the rab17 gene and indicate strong conservation of the seed-specific regulatory mechanism for rab genes in monocot and dicot plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00023554
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  • 4
    Electronic Resource
    Electronic Resource
    Regulation of the maizerab17 gene promoter in transgenic heterologous systems (1991)
    Springer
    Plant molecular biology 17 (1991), S. 985-993 
    Details
    ISSN: 1573-5028
    Keywords: rab17 promoter ; abscisic acid ; transgene expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The maizerab17 gene is expressed in different plant parts in response to ABA and osmotic stress (J. Vilardellet al., Plant Mol Biol 14 (1990) 423–432). Here we demonstrate that 5′ upstream sequences of therab17 gene confer the appropriate patterns of expression on the chloramphenicol acetyl transferase (CAT) reporter gene in transgenic tobacco plants, as well as in protoplasts derived from cultured rice cells. Specifically, a CAT construct containing a large 5′ upstream fragment ofrab17 (−1330/+29) results in high levels of CAT activity in embryos, leaves and roots of transgenic plants subjected to water stress or ABA treatment. Transient expression assays in rice protoplasts transfected with CAT genes fused torab17 promoter deletions indicate that a 300 bp DNA fragment (−351/−102) is sufficient to confer ABA responsiveness upon the reporter gene. Furthermore, a 100 bp sequence (−219/−102) is capable of conferring ABA responsiveness upon a minimal promoter derived from the 35S CaMV promoter. Gel retardation experiments indicate that maize nuclear proteins bind to this fragment. This region of 100 bp contains a sequence (ACGTGGC) which has been identified as an abscisic acid response element in studies of other ABA-responsive plant genes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00037138
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  • 5
    Electronic Resource
    Electronic Resource
    Differential regulation of ABA-induced 23–25 kDa proteins in embryo and vegetative tissues of the viviparous mutants of maize (1989)
    Springer
    Plant molecular biology 13 (1989), S. 385-394 
    Details
    ISSN: 1573-5028
    Keywords: ABA-induced proteins ; viviparous embryos ; water deficit
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Previous studies have identified a set of highly phosphorylated proteins of 23–25 kDa accumulated during normal embryogenesis of Zea mays L. and which disappear in early germination. They can be induced precociously in embryos by abscisic acid (ABA) treatment. Here the synthesis and accumulation of this group of proteins and their corresponding mRNAs were examined in ABA-deficient viviparous embryos at different developmental stages whether treated or not with ABA, and in water-stressed leaves of both wild-type and viviparous mutants. During embryogenesis and precocious germination of viviparous embryos the pattern of expression of the 23–25 kDa proteins and mRNAs closely resembles that found in non-mutant embryo development. They are also induced in young viviparous embryos by ABA treatment. In contrast, leaves of ABA-deficient mutants fail to accumulate mRNA in water stress, yet do respond to applied ABA. In water-stressed leaves of wild type plants the mRNAs are induced and translated into 4 proteins with a molecular weight and isoelectric point identical to those found in embryos. These results indicate that the 23–25 kDa protein set is a new member of the recently described class or proteins involved in generalized plant ABA responses. The different pattern of expression for the ABA-regulated 23–25 kDa proteins and mRNAs found in embryo and in vegetative tissues of viviparous mutants is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00015550
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  • 6
    Electronic Resource
    Electronic Resource
    Gene sequence, developmental expression, and protein phosphorylation of RAB-17 in maize (1990)
    Springer
    Plant molecular biology 14 (1990), S. 423-432 
    Details
    ISSN: 1573-5028
    Keywords: maize ; ABA-induced gene ; protein phosphorylation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The ABA-induced MA12 cDNA from maize, which encodes a set of highly phosphorylated embryo proteins, was used to isolate the corresponding genomic clone. This gene, called RAB-17 (responsive to ABA), encodes a basic, glycine-rich protein (mol. wt. 17 164) containing a cluster of 8 serine residues, seven of them contiguous. It is a homologue of the rice RAB-21 gene (Mundy J, Chua NH, EMBO J 7; 2279–2286, 1988). Phosphoamino acid analysis of the isolated protein indicates that only the serine residues are phosphorylated and a putative casein-type kinase phosphorylatable sequence was identified in the protein. The pattern of expression and in vivo phosphorylation of the RAB-17 protein was studied during maize embryo germination and in calli of both meristematic or embryonic origin. ABA treatment induced the synthesis of RAB-17 mRNA and protein in calli, however, the RAB-17 proteins were found to be highly phosphorylated only in embryos.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00028778
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