ISSN:
1573-2568
Keywords:
INTERFERON-γ
;
TUMOR NECROSIS FACTOR-α
;
INTERCELLULAR ADHESION MOLECULE-1
;
LYMPHOCYTE FUNCTIONASSOCIATED
;
ANTIGEN-1
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract Adhesions of leukocytes to hepatocytes andsinusoidal endothelial cells mediates the induction andprogression of hepatic injury. However, in contrast toendothelial cells, information regarding the regulation of interactions between leukocytes andhepatocytes is limited. In the present study, weinvestigated the effect of inflammatory mediatorsincluding lipopolysaccharide (LPS), staphylococcalenterotoxin B (SEB), interferon-γ (IFN-γ), tumornecrosis factor-α (TNF-α), andinterleukin-1β (IL-1β) on the adhesion ofpolymorphonuclear leukocytes or lymphocytes to primarycultured rat hepatocytes, and on the expression of intercellular adhesionmolecule-1 (ICAM-1) gene in hepatocytes. Bothpolymorphonuclear leukocyte and lymphocyte adhesion tohepatocytes were enhanced after exposure of hepatocytes to IFN-γ and TNF-α, but not afterexposure to LPS, SEB or IL-1β. The adhesion inducedby either IFN-γ or TNF-α was inhibited bymonoclonal antibodies against ICAM-1 or lymphocytefunction-associated antigen-1 (LFA-1). Nonstimulated hepatocytesexpressed faintly ICAM-1 mRNA, which increased slightlyduring the culture period. ICAM-1 mRNA expression wasup-regulated to a greater extent by incubating hepatocytes with IFN-γ or TNF-α,and peaked after 12 hr of incubation with TNF-αand after 24 hr with IFN-γ. These results indicatethat IFN-γ and TNF-α induce the expressionof ICAM-1 on parenchymal hepatocytes and that theLFA-1-ICAM-1 pathway plays an important role in theinteraction between hepatocytes and neutrophils orlymphocytes.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1026682329876
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