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  • 1
    Electronic Resource
    Electronic Resource
    Linear IgA disease with IgA antibodies directed against 200- and 280-kDa epidermal antigens (2000)
    Oxford, UK : Blackwell Science Ltd
    British journal of dermatology 142 (2000), S. 0 
    Details
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: We report an 80-year-old man with the lamina lucida type of linear IgA disease, with IgA autoantibodies reactive with 200-kDa and 280-kDa epidermal proteins. The patient presented with widespread bullous lesions on his trunk and extremities without mucosal involvement. Histopathology of lesional skin showed a subepidermal blister with papillary microabscesses of neutrophils and a few eosinophils. Direct immunofluorescence microscopy of perilesional skin showed linear deposits of IgA and C3 at the basement membrane zone. The patient’s serum contained IgA autoantibodies that bound exclusively to the epidermal side of 1 mol L−1 NaCl split skin as determined by indirect immunofluorescence microscopy. Circulating IgA autoantibodies to 200- and 280-kDa antigens were detected in the patient’s serum by immunoblot analysis using extracts from normal human epidermis and human epidermal keratinocytes. These two antibodies, eluted from individual nitrocellulose membranes, reacted with the epidermal side of 1 mol L−1 NaCl split skin on indirect immunofluorescence microscopy, and bound to hemidesmosomes as determined by immunoperoxidase electron microscopy. This observation suggests the presence of hitherto uncharacterized 200- and 280-kDa hemidesmosomal proteins distinct from BPAG1, BPAG2 and β4 integrin as target antigens in linear IgA disease.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1365-2133.2000.03553.x
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  • 2
    Electronic Resource
    Electronic Resource
    IRF-1 expression in normal human epidermal keratinocytes (1997)
    Springer
    Archives of dermatological research 289 (1997), S. 415-420 
    Details
    ISSN: 1432-069X
    Keywords: Key words IRF-1 ; ICAM-I ; Tryptophanyl tRNA ; synthetase ; IFN-γ ; Keratinocyte
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Interferon gamma (IFN-γ) is a potent inducer of cell growth arrest in human epidermal keratinocytes. Interferon regulatory factor-1 (IRF-1), an interferon-inducible gene, is a mediator of interferon action. It has also been suggested that IRF-1 may have a functional role as a tumor suppressor gene and may be associated with the antiproliferative effect of IFN-γ. We examined the expression of IRF-1 mRNA in normal human epidermal keratinocytes (NHEK). Northern blot analysis demonstrated an increased expression of IRF-1 mRNA by IFN-γ in NHEKs. This increased expression of IRF-1 mRNA by IFN-γ was not blocked by treatment with cycloheximide, but was abolished by treatment with actinomycin D. In addition, neither pretreatment with TPA, a protein kinase C (PKC) activator, nor treatment with H7, a PKC inhibitor, affected the induction of IRF-1 mRNA by IFN-γ. These results indicate that IFN-γ-induced IRF-1 mRNA in NHEKs is transcriptional and PKC-independent.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004030050214
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    Paper (German National Licenses)
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