Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    Human Cardiac Fibroblasts Express an Angiotensin Receptor with Unusual Binding Characteristics Which Is Coupled to Cellular Proliferation
    Amsterdam : Elsevier
    Biochemical and Biophysical Research Communications 204 (1994), S. 1334-1339 
    Details
    ISSN: 0006-291X
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1006/bbrc.1994.2609
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    7C. Effect of corticoids in in vitro preparations 267. ACTH and HGH production by isolated human adenohypophysis cells and hormone-active adenohypophysis tumour cells with and without stimulation and inhibition
    Amsterdam : Elsevier
    Journal of Steroid Biochemistry 5 (1974), S. 359 
    Details
    ISSN: 0022-4731
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0022-4731(74)90412-9
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Evidence that Borrelia burgdorferi immunodominant proteins p100, p94 and p83 are identical (1992)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 94 (1992), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract Recently there have been reports on high-molecular mass components of Borrelia burgdorferi, namely the p100, p94 and p83, which claimed these proteins to be specific marker antigens for the serodiagnosis of late Lyme borreliosis. The nucleotide sequences of the p100 and p83 have been published. The alignment of the deduced N-terminal amino acid sequences with the N-terminal sequence of the p94 now provides evidence that all three proteins are identical.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1992.tb05321.x
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Molecular biology of angiotensin receptors and their role in human cardiovascular disease (1996)
    Springer
    Journal of molecular medicine 74 (1996), S. 233-251 
    Details
    ISSN: 1432-1440
    Keywords: Angiotensin receptors types ; Cloning ; Signaling ; Transgenic models ; Regulation ; Transcription ; Organ specificity ; Human ; Cardiovascular ; Receptor antagonists
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The actions of angiotensin II in the cardiovascular system are transmitted by two known and possibly some unknown angiotensin receptor types. AT1 and AT2 both correspond to G-protein-coupled receptors with seven hydrophobic transmembrane domains, several N-glycosylation sites and a potential G-protein binding site. Cloning of coding regions and promoter sequences contributed to the understanding of receptor protein function and regulation. Angiotensin receptors with atypical binding properties for the known AT1- and AT2-specific ligands are expressed on human cardiac fibroblasts and in the human uterus. In several animal models, receptors with high affinity for angiotensin (1–7) have been described. AT1 stimulation is mediated by the generation of phospholipid-derived second messengers, activation of protein kinase C, the MAPkinase pathway and of immediate early genes. Recently, phosphorylation and dephosphorylation of tyrosine kinases have been associated with AT1- and AT2-mediated signal transduction. ATR are regulated by phosphorylation, internalization, modification of transcription rate and mRNA stability. Regulation is highly cell and organ specific and includes upregulation of ATR in some pathophysiological situations where the renin angiotensin system is activated. Whereas the function of AT1 in the cardiovascular system is relatively well established, there is little information regarding the role of AT2. Recent hypotheses suggest an antagonism between AT1 and AT2 at the signal transduction and the functional level. Transgenic animal models, particularly with targeted disruption of the AT1 and AT2 genes, suggest the contribution of both genes to blood pressure regulation. Genetic polymorphisms have been described in the AT1 and AT2 gene or neighbored regions and are used to analyze the association between gene defects and cardiovascular diseases. AT1 antagonists are now being introduced into the treatment of hypertension and potentially heart failure, and more interesting pharmacological developments are expected from the ongoing basic studies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00196577
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    Orientation of displaced homing pigeons with shifted Circadian clocks: Prediction vs observation (1988)
    Springer
    Naturwissenschaften 75 (1988), S. 363-365 
    Details
    ISSN: 1432-1904
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00368332
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    Prostaglandins: Antiproliferative effect of PGD 2 on cultured human glioma cells (1986)
    Springer
    Acta neurochirurgica 83 (1986), S. 56-61 
    Details
    ISSN: 0942-0940
    Keywords: Prostaglandin analogues ; thymidine uptake ; flow cytometry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Five cultured human glioma cell lines were investigated for their reaction to prostaglandin (PG) D 2 and E 2. In all cases a suppressive effect on DNA synthesis as assessed by3H-thymidine incorporation was seen with all test substances as early as six hours after the addition of the compounds in doses of usually 10−5 M. A dose response curve was generated in four cases and showed an estimated ED 50 of about 5 · 10−6 M. The effect was most pronounced at 12 hours after which the cultures began to recover except those which had been incubated with PGD 2. In those cultures which had been exposed to PGD 2 virtually no thymidine incorporation was seen after 24 hours and as long as 72 hours. In another set of experiments, the effect of PGD 2, PGE 2, two synthetic PGD 2 analogues, with a chlorine substitution in position 9 (DACl) or with a fluoride substitution in position 9 (DAF) and a synthetic prostacyclin-analogue (Iloprost) was investigated after single and repeated addition of the compounds. A second administration after 12 hours of incubation did not result in a further decrease in3H-thymidine incorporation like that observed during that first incubation period. In general the cells recovered after 24 hours total incubation time except those which had received PGD 2 or repeated doses of PGE 2. Only in those cells which had been treated with PGD 2, an almost complete blockade of3H-thymidine incorporation was seen even after the single administration. Parallel evaluation of the cells by flow cytometry showed effects on cell cycle distribution at different times of the incubation. After 12 hours cells began to accumulate in G2/M at levels of approximately twice control, the effect being the least pronounced for PGD 2. For this compound we observed a threefold increase in cells in the S phase. After 24 hours the cell cycle distribution had normalized for all compounds except PGD 2 where the “arrest” of cells in G2/M persisted to be about 2–3-fold control level until the end of the experiment. Our data suggest, that also in cultured human glioma cells, prostaglandins are effective in suppressing cellular DNA synthesis. Although the effect of PGD 2 can be achieved to some extent by PGE 2 and the analogues which are more stable to dehydrogenases and the metabolic conversion into other biologically active homologues, PGD 2 appears to have a unique quality of action, becoming apparent 24 hours after administration.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01420509
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 7
    Electronic Resource
    Electronic Resource
    Does the timing of aneurysm surgery neglect the real problems of subarachnoid haemorrhage? (1987)
    Springer
    Acta neurochirurgica 89 (1987), S. 91-99 
    Details
    ISSN: 0942-0940
    Keywords: Subarachnoid haemorrhage ; ruptured cerebral aneurysms ; cerebral vasospasm ; nimodipine ; timing of surgery ; treatment mortality ; morbidity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In 1984, in connection with the introduction of the calcium antagonist nimodipine, a new strategy for the treatment of subarachnoid haemorrhage (SAH) due to ruptured aneurysm was developed in our hospital. With no rigid regard to “timing” all patients undergo surgery as soon as possible. The only exception being those in Hunt and Hess grades IV and V without space-occupying intracranial haemorrhage and those bearing aneurysms of the vertebrobasilar circulation that are difficult of access. As soon as the risk of rebleeding has been eliminated surgically an active therapy against the possible consequences of SAH—cerebral vasospasm and simultaneous disturbances of autoregulation—is started. It consists in lowering the increased intracranial pressure, raising of mean arterial pressure and improving of rheological properties of the blood in order to prevent delayed build-up of neurological deficit due to ischaemia. It goes without saying that calcium antagonists are given from the very beginning of the patient's treatment even before operation. The advantages of this therapeutic concept are demonstrated by two series of non-selected consecutive patient material. The first series (A; n=135) was treated between 1981 and 1984 before the change in treatment strategy, the second (B; n=183) from 1984 to 1986 after that change. The overall mortality in series A was 27%, that in series B 20%. Operative mortality could be reduced from 22% to 16% in patients having undergone early operation and from 6% to 2% in patients with late surgery. Development of permanent neurological deficits following early surgical intervention was seen in 4 out of 29 grade I–III patients (14%) in series A and in 5 out of 94 of such patients (5%) in series B.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01560372
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 8
    Electronic Resource
    Electronic Resource
    Isolation and characterisation of human cardiac fibroblasts from explanted adult hearts (1996)
    Springer
    Cell & tissue research 286 (1996), S. 145-153 
    Details
    ISSN: 1432-0878
    Keywords: Key words: Cardiac fibroblasts ; Extracellular matrix proteins ; Angiotensin II ; Angiotensin receptor ; Ageing ; Human
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. Fibrosis makes an important contribution to the pathophysiological events leading to the development of heart failure in ischemic and hypertensive heart disease. Since cardiac fibroblasts are mainly responsible for the synthesis and deposition of the extracellular matrix, we have established a method for isolating and cultivating human cardiac fibroblasts from explanted human hearts. The cell yield was 2.14±0.25×106 cells in five independent isolations and the cell purity was 95–97%, contaminating cells being vascular smooth muscle cells and pericytes. Cultured cells were studied with respect to growth properties, morphology and deposition of components of the extracellular matrix. Isolated cells displayed a differentiated phenotype, including the second passage in culture; they synthesised collagen I, III, IV, fibronectin, vitronectin, tenascin and chondroitin sulphate and expressed an atypical angiotensin receptor. This atypical angiotensin receptor internalised angiotensins II and III but not angiotensin IV in a time-dependent manner. Stimulation of the cells with angiotensins II and III but not with angiotensin IV resulted in a dose-dependent stimulation of DNA synthesis. Co-incubation with the subtype-specific receptor antagonists Losartan and PD 123317 did not prevent the stimulation of DNA synthesis. The further characterisation of this receptor should provide insights into the pathobiochemical events leading to heart failure in hypertension and ischemic heart disease.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004410050683
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 9
    Electronic Resource
    Electronic Resource
    Optionen der postpartalen Therapie bei pränatal diagnostizierten Herzfehlern (1997)
    Springer
    Der Gynäkologe 30 (1997), S. 260-269 
    Details
    ISSN: 1433-0393
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Zusammenfassung Viele Herzerkrankungen können heute im Rahmen von Schwangerschaftsvorsorgeuntersuchungen frühzeitig diagnostiziert werden. So ist in vielen Fällen, besonders bei Herzrhythmusstörungen, eine kurative pränatale Therapie möglich. Bei strukturellen Herzfehlern ist eine Therapie jedoch, abgesehen von der Behandlung einer möglichen Herzinsuffizienz oder begleitender Herzrhythmusstörungen, häufig erst nach der Geburt möglich. Ist der Herzfehler bereits pränatal erkannt, so kann das herzkranke Neugeborene ohne Zeitverzögerung in einer kinderkardiologischen Klinik weiter betreut werden. Neben medikamentösen Behandlungsmöglichkeiten ist in vielen Fällen eine frühzeitige interventionelle und/oder chirurgische Therapie für die Prognose des herzkranken Neugeborenen besonders wichtig. Im folgenden werden aktuelle Behandlungsmethoden für angeborene Herzfehler anhand einiger typischer Beispiele mit besonderer Berücksichtigung von herzkranken Neugeborenen und Säuglingen aufgezeichnet.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/PL00003035
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 10
    Electronic Resource
    Electronic Resource
    Subtype 2 and atypical angiotensin receptors in the human heart (1996)
    Springer
    Basic research in cardiology 91 (1996), S. 73-77 
    Details
    ISSN: 1435-1803
    Keywords: Angiotensin receptors ; subtypes ; fibroblasts ; AT2 promoter ; transcription
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Angiotensin receptors have been described in the human heart and are suspected to play a central role in remodeling after myocardial infarction and in cardiac hypertrophy. Two subtypes, AT1 and AT2, have so far been described in humans, with AT2 being the dominant subtype in human atria. We have now determined subtype numbers and distribution by binding in ventricular myocardium from patients with end-stage heart failure. We found about 50–80% of subtype AT2 in the right and left ventricles from patients with end-stage heart failure due to coronary artery disease and cardiomyopathy, indicating that AT2 is the dominant angiotensin receptor subtype in the whole human heart. To determine the cellular localization of angiotensin receptors in human myocardium in addition to the known localization on myocytes, smooth muscle cells and endothelial cells, we investigated cardiac fibroblasts. They express an angiotensin receptor with yet incompletely understood binding characteristics which is coupled to proliferation and DNA synthesis. As AT2 is the dominant angiotensin receptor subtype in human heart, we cloned the complete mRNA sequence by a rapid amplification of cDNA ends (RACE) procedure and thereafter the promoter sequence from a human genomic library. Once the sequence of the mRNA and thus exon 1 was obtained by the RACE-PCR, a probe was constructed for the most 5′ region of exon 1 and used for screening of a human genomic DNA bank. After cutting of the positive clones with EcoR1 and Not1, a 4000 bp fragment hybridized with the probe and was further sequenced. A functional AT2 promoter, with 〉90% homology with the mouse promoter and 35% homology with the human AT1 promoter containing numerous cis-acting sequences for basal (TFIID) and inducible (AP-1, PEA-3, CBF) transcription factors in the first 1000 bp was identified.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00795366
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...