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  • 1
    ISSN: 1824-3096
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Tropical animal health and production 20 (1988), S. 269-270 
    ISSN: 1573-7438
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1573-7446
    Keywords: Brucella abortus ; cattle ; chelating agents ; EDTA ; EGTA ; ELISA ; immunoassay ; specificity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract An indirect enzyme immunoassay (ELISA) for detection of bovine antibody toBrucella abortus was modified by the addition of divalent chelating agents to the serum diluent. This addition resulted in an increase in specificity from 96.0% in the regular assay to 99.4% in the modified procedure. Of the 15 715 sera initially tested by the indirect ELISA, 691 that had given positive reactions were selected for retesting in the indirect ELISA with EDTA/EGTA added. The buffered plate antigen test (BPAT) correctly identified 98.6% of the samples as negative. The addition of chelating agents did not alter the sensitivity of the indirect ELISA, which correctly classified 609 sera from animals from whichB. abortus had been isolated as positive. The sensitivity of the BPAT was 97.8%.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1573-7438
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Résumé Au Kuwait, environ 12,000 vaches laitiěres ont été vaccinées avec une dose réduite de souche 19Brucella abortus (3×109) et environ 350,000 moutons et chevres qui étaient matures sexuellement, one été vaccinés avec une dose réduite de soucheB. melitensis Rev. 1 (107). Basé sur la présence d'anticorps, sur le nombre d'avortements et de cas de brucellose chez les humains avant et aprés vaccination, le programme a été un succěs. La méthode la plus effective pour contrôler la brucellose chez les bovins, les ovins et les caprins dans beaucoup de pays et de vacciner sans exception tous les animaux adultes.
    Abstract: Resumen En Kuwait, se vacunaron aproximadament 12,000 vacas lecheras con una dosis reducida de 3×109 organismos deBrucella abortus cepa 19 y approximadament 350,000 ovejas y cabras sexualmente maduras fueron vacunadas con una dosis reducida de 107 organismos deB. melitensis opa Rev. 1. Utilizando los criterios prevacunales de incidencia de anticuerpos, abortos, y casos humanos de brucelosis, el programa fuvo gran exito. Ef método mas efectivo de control de la brucelosis bovina, ovina y caprina an numerosas paises es la amplia utilización de vacuna en animales adultos.
    Notes: Summary In Kuwait, approximately 12,000 diary cows were vaccinated with a reduced dose of 3×109 Brucella abortus strain 19 and approximately 350,000 sexually mature sheep and goats with a reduced dose of 107B.melitensis strain Rev. 1. Using the criteria of prevaccinal and postvaccinal incidences of antibodies, abortions, and human cases of brucellosis, the programme was very successful. Widespread vaccination of adult animals is the most effective method of controlling brucellosis among cattle, sheep and goats in many countries.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Tropical animal health and production 17 (1985), S. 82-82 
    ISSN: 1573-7438
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    European journal of epidemiology 9 (1993), S. 335-340 
    ISSN: 1573-7284
    Keywords: Urinary tract infection ; Gram-negative ; Gram-positive ; Domiciliary bacteriuria ; Nosocomial bacteriuria
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Our study of significant bacteriurias indicated that the worldwide shift in the etiology of infections also holds true for the Florence area. In a twenty-year period (1970–1990), we noted a decreased frequency of Gram-negative bacilli, particularly of the family Enterobacteriaceae, and a significant increase of Gram-positive cocci in urinary patients. This finding was observed both in hospital and in community-acquired cases in the male sex and only in nosocomial bacteriurias in the female sex. There was a reduced isolation of “classic” urinary pathogens such as Proteus mirabilis: its prevalence in hospital-acquired urinary tract infection (UTI) decreased from 16% in 1970 to 5% in 1990 both in males and in females. On the other hand, we noted an increase of “difficult” microorganisms such as enterococci and methicillin-resistant staphylococci, particularly in the male sex; in 1970 enterococci were occasionally isolated in males both from hospital and from community-acquired UTIs (3% and 5%, respectively), whereas in 1990, on the contrary, they were encountered much more frequently (19% in both cases).
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1435-4373
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  To assess the spread of the new M phenotype, various erythromycin-resistant Streptococcus pyogenes strains from three Italian cities (Verona, Monza, Florence) were characterised. Each strain was analysed for the presence of genes ermAM and mefA, for the ability to accumulate radioactive erythromycin in the absence of sodium arsenate, for the protein T serological type, and for the DNA macrorestriction profile identified by means of pulsed-field gel electrophoresis. In a number of strains, the presence of the inducible ermAM gene was demonstrated; all these strains were negative in the efflux-pump detection assay, did not possess the mefA gene, and had similar restriction profiles. The strains with the efflux mechanism and mefA gene belonged to different serotypes. Of these, only one serotype, T4, was isolated in all three cities. The restriction profile analysis with SmaI and SfiI revealed a very close correlation between strains with the same serotype.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    European journal of clinical microbiology & infectious diseases 14 (1995), S. 1105-1108 
    ISSN: 1435-4373
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The new thiazolyl peptide antibiotic MDL 62,879 (GE2270 A) showed excellent in vitro activity in testing against staphylococci and streptococci, with MIC90s ranging from 0.23 to 0.9 mg/l. It was very active againstClostridium difficile andPropionibacterium acnes (MIC90 0.06 mg/l in each case) and had variable activity againstBacteroides spp. MDL 62,879 had exceptionally good activity againstEnterococcus faecalis, including against a collection of high-level aminoglycoside-resistant isolates where it had an MIC90 of 0.047. The antibiotic was bacteriostatic for enterococcal isolates but bactericidal for a methicillin-resistant isolate ofStaphylococcus aureus.
    Type of Medium: Electronic Resource
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