ISSN:
1573-5060
Keywords:
PCR-RFLP
;
Raphanus sativus
;
S-allele
;
self-incompatibility
Source:
Springer Online Journal Archives 1860-2000
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
Notes:
Abstract From 16 inbred lines of cultivated radishes (Raphanus sativus L.), 6 S-alleles tentatively named S201 to S206 were identified, and their dominance relationships were examined. Among the S-alleles, S201, S202, S203 and S204 were found to be co-dominant. These 4 S-alleles showed dominance with S205 in pollen and with S206 in both pollen and stigma, while S205 and S206 were co-dominant. Polymerase chain reaction (PCR) was performed using the radish inbred lines randomly selected from the 6 S-allele groups. The primers were based on the highly conserved sequences of the S-locus specific glycoprotein (SLG) genes in Brassica oleracea. As a result of the PCR, a single DNA fragment of about 1.16kb was amplified as expected from the original sequence of B.oleracea. The S-allele specific pattern in the restriction fragments of the PCR products (PCR-RFLP) was confirmed for the first group of S-alleles (S201, S202, S203 and S204). However, for the second group of the S-alleles (S205 and S206), no PCR products were obtained. The usefulness of the PCR-RFLP in a radish breeding program is described.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1018313801728
Permalink
