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  • 1
    Electronic Resource
    Electronic Resource
    Tubulin and actin mRNAs in the young-adult and the aged rat brain: effects of repeated administration with bifemelane hydrochloride
    Amsterdam : Elsevier
    Archives of Gerontology and Geriatrics 19 (1994), S. 265-272 
    Details
    ISSN: 0167-4943
    Keywords: Actin ; Aging ; Bifemelane hydrochloride ; Cytoskeletal protein ; Rat brain ; Tubulin
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Medicine
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0167-4943(94)00572-9
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  • 2
    Electronic Resource
    Electronic Resource
    Visualization of DNA in various phages (T4, χ, T7, ϕ 29) by ethidium bromide epi-fluorescent microscopy (1981)
    Springer
    Cellular and molecular life sciences 37 (1981), S. 969-971 
    Details
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary DNA-containing areas in various phages (T4, χ, T7 and ϕ29) could be observed at the light microscopic level using ethidium bromide epi-fluorescent microscopy. The fluorescent intensity per phage was in linear proportion to the DNA content in each phage.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01971784
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  • 3
    Electronic Resource
    Electronic Resource
    Variance of ploidy in mitochondrial nucleus during spherulation in Physarum polycephalum
    Amsterdam : Elsevier
    Experimental Cell Research 149 (1983), S. 359-373 
    Details
    ISSN: 0014-4827
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0014-4827(83)90349-X
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  • 4
    Electronic Resource
    Electronic Resource
    Somatic hybrids between Brassica oleracea and B. campestris: selection by the use of iodoacetamide inactivation and regeneration ability (1987)
    Springer
    Theoretical and applied genetics 73 (1987), S. 379-384 
    Details
    ISSN: 1432-2242
    Keywords: Brassica ; Protoplast fusion ; Somatic hybrid ; Selection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary An efficient procedure for obtaining somatic hybrids between B. oleracea and B. campestris has been developed. Hypocotyl protoplasts of B. oleracea were fused with mesophyll protoplasts from three different varieties of B. campestris by the polyethylene glycoldimethylsulfoxide method. The selection of somatic hybrids utilized the inactivation of B. oleracea protoplasts by iodoacetamide (IOA) and the low regeneration ability of B. campestris. The efficiency of recovery of somatic hybrids depended upon the IOA concentration, and when 15 mM IOA was used, 90% of the regenerated plants were found to be hybrid. The somatic hybrids were examined for i) leaf morphology, ii) leucine aminopeptidase (LAP) isozyme and iii) chromosome number. All the hybrids had intermediate leaf morphology and possessed LAP isozymes of both parental species. The chromosome analysis revealed a considerable variation in chromosome number of somatic hybrids, showing the occurrence of multiple fusion and chromosome loss during the culture. Some of the hybrids flowered and set seeds.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00262504
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  • 5
    Electronic Resource
    Electronic Resource
    CMV protecton in transgenic cucumber plants with an introduced CMV-O cp gene (1996)
    Springer
    Theoretical and applied genetics 93 (1996), S. 672-678 
    Details
    ISSN: 1432-2242
    Keywords: CMV-cp gene ; Transgenic cucumber plants ; CMV resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We introduced the CMV-O coat-protein gene into cucumber plants, using a Ti-Agrobacterium-mediated transformation system, with the aim of producing cucumber plants with CMV resistance. The RNA transcripts from the CaMV 35s-cp gene could be detected in the leaves of the R0 transgenic cucumber plants, as well as in the epicotyls containing two cotyledons of transgenic progeny plants, by Northern-blot analysis; but the presence of coat protein originating from the CaMV 35s-cp gene could not be detected in the cotyledons or leaves of R0 and transgenic progeny plants by Westernblot analysis. The progenies of a cross between cv “Sharp 1” and transgenic plants (pure line “1021”) possessing the cp gene displayed strong resistance to inoculation of the CMV-Y strain, although both the control cv “Sharp 1” and segregated cp - plants displayed many spotted disease symptons on their leaves 5–6 days after CMV-Y inoculation on the cotyledons. The control “1021” had a slight tolerance toward CMV-Y inoculation. The transgenic cucumber plants displayed the absence of resistance to ZYMV. However, transgenic plants showed a reduced degree of disease symptom development following a double inoculation of CMV and ZYMV. The CMV resistance of the present transgenic cucumber plants seems to be due to the synergism of the slight CMV tolerance in the pure line “1021” and the protection against CMV afforded by the introduction of the CMV cp gene. This leads to the possibility of producing cucumber plants with the agronomic characteristics of very strong CMV resistance by the combination of genotypes of cucumbers and the CMV cp gene. The transgenic plants possessing the cp gene should thus be useful as a genetic source for producing cucumber plants with the agronomic characteristic of CMV resistance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224061
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  • 6
    Electronic Resource
    Electronic Resource
    Asymmetric somatic hybrids of Brassica: partial transfer of B. campestris genome into B. oleracea by cell fusion (1989)
    Springer
    Theoretical and applied genetics 77 (1989), S. 189-194 
    Details
    ISSN: 1432-2242
    Keywords: Brassica ; Protoplast fusion ; Asymmetric hybrid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary To examine the possibility of producing asymmetric somatic hybrids of Brassica having a complete genome of one species and a part of the other, we fused inactivated B. oleracea protoplasts with X-irradiated B. campestris protoplasts. The plants obtained were studied with regard to their morphology, isozymes and chromosomes. The morphology of the hybrids was similar to B. oleracea in 9 out of 22 hybrids studied and the rest showed the intermediate phenotype of the parents. Analysis of three isozymes, leucine aminopeptidase, acid phosphatase and esterase indicated that ten hybrids lost B. campestris-specific bands in one or more of the three isozymes examined. The chromosome analysis showed that 90% of the hybrids were aneuploids. In addition, abnormal chromosomes were often found in root tip cells. These results suggested that the hybrids obtained were asymmetric in nature and resulted from elimination of B. campestris chromosomes by X-ray irradiation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00266185
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  • 7
    Electronic Resource
    Electronic Resource
    Behavior of leucoplast nucleoids in the epidermal cell of onion (Allium cepa) bulb (1982)
    Springer
    Protoplasma 110 (1982), S. 177-184 
    Details
    ISSN: 1615-6102
    Keywords: Leucoplast division cycle ; Leucoplast nucleoid ; DAPI ; Allium cepa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The behavior of nucleoids during the leucoplast division cycle in the epidermis of onion (Allium cepa) bulbs was investigated using DNA-specific fluorochrome 4'6-diamidino-2-phenylindole (DAPI) staining. The leucoplast was morphologically amoeboid and continuously changed its shape. A dumbbell-shaped leucoplast divided into two spherical daughter ones by constriction in the middle region of the body. Leucoplasts contained 4–10 mostly spherical, oval, partly rodand dumbbell-shaped nucleoids which were dispersed within the bodies. The proportion of one DNA molecule of a T4 phage particle to the small leucoplast nucleoid in the grain density of negative film was 1 to 0.91. Comparison of the present result and another groups' biochemical results suggested that a small leucoplast nucleoid contains one DNA molecule. The dumbbell-shaped leucoplast probably before division contained about twice as many nucleoids as the spherical leucoplast after division, and each half of the dumbbell contained about half the number of nucleoids. Nucleoids increased in number with growth of the leucoplast. The behavior of nucleoids during the leucoplast division cycle in onion bulbs was basically similar to that during the chloroplast division cycle in higher plants and green algae, which was previously reported (Kuroiwa et al. 1981 b).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01283320
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  • 8
    Electronic Resource
    Electronic Resource
    Acetylcholinesterase inhibitor ENA-713 protects against ischemia-induced decrease in pre- and postsynaptic cholinergic indices in the gerbil brain following transient ischemia (1994)
    Springer
    Neurochemical research 19 (1994), S. 117-122 
    Details
    ISSN: 1573-6903
    Keywords: Acetylcholinesterase inhibitor ; ENA-713 ; cholinergic indices ; transient ischemia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The effects of pre-treatment with ENA-713, an acetylcholinesterase (AChE) inhibitor, on changes in pre- and postsynaptic cholinergic indices in gerbil brain following transient ischemia were studied at 4 and 14 days after recirculation. In the ischemic group, hippocampal acetylcholine (ACh) level was significantly reduced (to 23% of sham-operated controls) at 4 days post-ischemia, but this reduction was completely prevented by ENA-713 treatment. Choline acetyltransferase (ChAT) and cholinesterase (ChE) activities were not significantly changed at 4 and 14 days post-ischemia. Although the maximum number (Bmax) of muscarinic ACh receptor (mACh-R) binding in the hippocampus was decreased (to 44%) without any change in affinity at 14 days post-ischemia, this decrease was also inhibited by ENA-713 treatment. In addition, histological experiment indicated that ENA-713 inhibited ischemia-induced pyramidal cell loss in the hippocampal CA1 regions. Thus, these findings suggest that ENA-713 has protective, neurotrophic and therapeutic effects on cerebrovascular type dementia due to cerebral ischemia.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00966804
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