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  • 1
    Electronic Resource
    Electronic Resource
    Bullous pemphigoid in a patient with a C4 deficiency (1991)
    Oxford, UK : Blackwell Publishing Ltd
    British journal of dermatology 124 (1991), S. 0 
    Details
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: A 53-year-old man with bullous pemphigoid with an extremely low level of C4 in the serum is reported.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2133.1991.tb00579.x
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  • 2
    Electronic Resource
    Electronic Resource
    Purification and partial characterization of aldehyde dehydrogenase from human erythrocytes
    Amsterdam : Elsevier
    BBA - Enzymology 569 (1979), S. 117-123 
    Details
    ISSN: 0005-2744
    Keywords: (Human erythrocyte) ; Acetaldehyde ; Aldehyde dehydrogenase ; Disulfiram
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0005-2744(79)90046-9
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  • 3
    Electronic Resource
    Electronic Resource
    Activation of human complement by rat peritoneal mast cells and its inhibition by a rat serum factor (1988)
    Springer
    Inflammation research 24 (1988), S. 73-79 
    Details
    ISSN: 1420-908X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The effects of normal sera from humans, rats, and guinea pigs on unsensitized rat peritoneal mast cells were studiedin vitro. Five to 20% fresh human sera induced mast cell death and substantial histamine release. The factor was heat labile. Neither hereditary C3-deficient sera nor experimentally Clq-depleted sera showed cytotoxicity. The CH50 activity of human serum was decreased to about one half after a 15-min incubation with 2×106 mast cells/ml at 37°C. The cytotoxic activity and CH50 reduction were completely eliminated by an addition of 10 mM Mg-EGTA to the serum. These data demonstrated that unsensitized rat mast cells served as both the initiator and target of complement activity when human serum was used as a complement source. Requirements of both Ca++ and Clq suggested the activation of the classical pathway of complement. Fresh 5–20% sera from rats and guinea pigs, on the other hand, showed neither cytotoxicity nor CH50 reduction. Furthermore, these sera strongly inhibited the human serum-induced reaction. The latter results indicated the presence of a modulating factor in rat and guinea pig sera, which inhibits mast cell associated complement activation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01968082
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Application of immunoblotting to serum protein phenotyping with reference to α2HS-glycoprotein (AHS) typing of bloodstains (1985)
    Springer
    International journal of legal medicine 95 (1985), S. 153-158 
    Details
    ISSN: 1437-1596
    Keywords: Serum groups, immunoblotting ; α2HS-glycoprotein typing ; Bloodstains, immunoblotting ; Serumgruppen, Immunoblottierung ; α2HS-Glykoprotein, Typisierung ; Blutspuren, Immunoblottierung
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Law
    Description / Table of Contents: Zusammenfassung Seren und Blutextrakte wurden auf Polyacrylamidgelen isoelektrisch fokussiert. Die fokussierten Proteine wurden auf Nitrozellulosemembranen durch Diffusion oder elektrophoretisch transferiert. Durch ein spezifisches Antiserum erfolgte auf den Membranen die AG-AK-Reaktion. Nach Waschen der Membranen wurde der gebundene Antikörper mit einem enzymisch markierten Anti-Kaninchen IgG-Antikörper nachgewiesen. Mit dieser Technik konnten die gruppenspezifische Komponente, das α2HS-Glykoprotein, die Komplementkomponenten C6 und C7, der Gerinnungsfaktor 13B und das Plasminogen sehr empfindlich phänotypisiert werden. Vorzugsweise konnten bis zu 6 Monate alte Blutspuren richtig typisiert werden.
    Notes: Summary Sera and bloodstain extracts were subjected to isoelectric focusing in polyacrylamide gels. The focused proteins were transferred to nitrocellulose membranes by diffusion or electrophoretically, then allowed to react with specific antiserum and, after washing, with peroxidase-labeled anti-rabbit IgG. The immune complexes formed on the membranes were detected with 4-chloro-1-naphthol and hydrogen peroxide. Serum group-specific component, α2HS-glycoprotein, the sixth and the seventh component of complement, factor 13B, and plasminogen could be phenotyped with high sensitivity. Bloodstains as old as 6 months could be correctly typed for α2HS-glycoprotein by the blotting technique.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00201072
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  • 5
    Electronic Resource
    Electronic Resource
    Genetic polymorphism of the B subunit of human coagulation factor XIII: Another classification (1981)
    Springer
    Human genetics 〈Berlin〉 59 (1981), S. 360-364 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Genetic polymorphism of the B subunit of human coagulation factor XIII was studied using agarose gel isoelectric focusing (pH 4–6.5) followed by immunofixation. Factor XIII-B of all samples after desialylation was classified into three types (F, S, and FS). From results of the present study, it was confirmed that factor XIII-B was controlled by two codominant alleles on an autosomal locus. Allele frequencies of F-XIIIB F and F-XIIIB S in a Japanese population were 0.336 and 0.664, respectively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00295472
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    Simultaneous Phenotyping of Genetic Markers for Paternity Testing (1987)
    Springer
    International journal of legal medicine 99 (1987), S. 135-142 
    Details
    ISSN: 1437-1596
    Keywords: Simultaneous phenotyping, genetic markers ; Isoelectric focusing, electrophoresis ; Immunoblotting, paternity testing ; Phänotypisierung genetischer Marker ; Vaterschaftsbegutachtung, kostensparende Methoden und Dokumentation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Law
    Description / Table of Contents: Zusammenfassung Es werden zeit- und kostensparende Methoden für die Vaterschaftsbegutachtung beschrieben. Siebzehn genetische Systeme werden in sechs Gruppen unterteilt. 1. Gruppe: Transferrin (Tf), Faktor B (BF) und Phosphoglucomutase 1 (PGM1); 2. Gruppe: Gc-System (Gc) oder α1-Antitrypsin (PI) und α-2HS-Glycoprotein (HSGA); 3. Gruppe: Komplement-Komponente C6 und C7, Faktor 13B (F13B) und Plasminogen (PLG); 4. Gruppe: Haptoglobin (Hp), C8 α-γ Kette (C81) und Faktor I (IF); 5. Gruppe: saure Erythrozyten-Phosphatase (ACP), Esterase D (ESD), Glutamat-Pyruvat-Transaminase (GPT); 6. Gruppe: 6-Phosphogluconat Dehydrogenase (PGD) und Glyoxalase I (GLO). Jede Gruppe wurde gleichzeitig mittels Elektrophorese oder isoelektrische Fokussierung (IEF) mit Färbung oder Immunoblotting untersucht. Diese Methoden erwiesen sich in der Praxis als zeit- und kostensparend und erleichtern die vorübergehende Aufbewahrung und Dokumentation der Elektrophorese-Bilder.
    Notes: Summary Time- and cost-saving methods for paternity testing are described. Seventeen genetic systems were divided into six groups: (1) transferrin (Tf), factor B (Bf), and phosphoglucomutase 1 (PGM1); (2) group-specific component (Gc) or α1-antitrypsin (PI) and α2HS-glycoprotein (HSGA); (3) complement components C6 and C7, factor 13B (F13B), and plasminogen (PLG); (4) haptoglobin (Hp), C8 α-γ chain (C81), and factor I (IF); (5) red cell acid phosphatase (ACP), esterase D (ESD), and glutamic-pyruvic transaminase (GPT); and (6) 6-phosphogluconate dehydrogenase (PGD) and glyoxalase I (GLO). Each group of systems was typed simultaneously by electrophoresis or isoelectric focusing (IEF) followed by staining or immunoblotting. These methods are very practical because they afford a considerable saving of time, work and expense, and facilitate semipermanent preservation of electrophoretic patterns.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00200633
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  • 7
    Electronic Resource
    Electronic Resource
    Genotyping of the ABO blood group system: analysis of nucleotide position 802 by PCR-RFLP and the distribution of ABO genotypes in a German population (1996)
    Springer
    International journal of legal medicine 109 (1996), S. 90-93 
    Details
    ISSN: 1437-1596
    Keywords: ABO blood group ; Genotyping PCR-RFLP ; German population
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Law
    Notes: Abstract Genotypes of the ABO blood group system were studied by PCR-RFLP analysis of the eight polymorphic nucleotide positions (ups) 261, 467, 526, 646, 703, 796, 802 and 803 of the cDNA from A transferase. In 169 unrelated German individuals, 17 genotypes were found and the calculated allele frequencies of A(Pro), A(Leu), B, O(T), O(A) and O2 were 0.2130, 0.0770, 0.0473, 0.4260, 0.2160 and 0.0207, respectively. These frequency data may provide useful additional information for disputed paternity and stain testing. A variant O allele, O2, was fout at a polymorphic frequency. As the nucleotide (np 261) of the O2 allele is the same as that of A and B alleles, the analysis of at least three nucleotide positions, i.e. ups 261, 526 and 802, is necessary to avoid mistyping of the ABO genotype.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01355523
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