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  • 1
    Electronic Resource
    Electronic Resource
    [S.l.] : American Institute of Physics (AIP)
    Physics of Plasmas 9 (2002), S. 171-176 
    ISSN: 1089-7674
    Source: AIP Digital Archive
    Topics: Physics
    Notes: An n=1 mode global motion on a field-reversed configuration (FRC) plasma is observed by means of a newly developed optical system. The deviation of the FRC from the coil axis reaches 20%–40% of the plasma radius. In order to push back the FRC to the equilibrium position, a multipole field (quadrupole or hexapole field) is applied. The n=1 motion can be easily controlled by the quadrupole field, the critical field strength of which is required to be about 15% of the confinement field. It is found that the n=2 rotational instability can also be stabilized by strength of the same order. The critical strength for the n=1 motion is theoretically obtained from a model such that the driving energy of the motion given at the formation phase balances with the work done by the multipole field. The theoretical estimation agrees within a factor of 2 with the experimental results. © 2002 American Institute of Physics.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Biochemical and Biophysical Research Communications 170 (1990), S. 470-476 
    ISSN: 0006-291X
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-0983
    Keywords: Aneuploid ; S. cerevisiae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary An aneuploid strain of Saccharomyces cerevisiae was isolated from a haploid strain with gal11, mutation of which would presumably occur through mitotic nondisjunction. The strain carries an extra copy of chromosome II deleted for a distal part of its left arm covering ISL1. The partial chromosome is stably inherited in repeated meioses. The aneuploid strain is also mitotically stable and remains disomic for more than 99.5% of the time. The transfer-hybridization experiment with radioactively labelled λ phage DNA containing yeast GAL genes as a probe indicated that the aneuploid strain contains two copies of those genes.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1617-4623
    Keywords: Key words Fission yeast ; RNA polymerase I ; Two-hybrid system ; α-Subunit
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Eukaryotic RNA polymerases I and III share two distinct α-related subunits that show limited homology to the α subunit of Escherichia coli RNA polymerase, which forms a homodimer to nucleate the assembly of prokaryotic RNA polymerase. To gain insight into the functions of α-related subunits in eukaryotes, we have previously identified the α-related small subunit RPA17 of RNA polymerase I (and III) in Schizosaccharomyces pombe, and have shown that it is a functional homolog of Saccharomyces cerevisiae AC19. In an extension of that study, we have now isolated and characterized rpa42 +, which encodes the α-related large subunit RPA42 of S. pombe RNA polymerase I, by virtue of the fact that its product interacts with RPA17 in the yeast two-hybrid system. We have found that rpa42 + encodes a polypeptide with an apparent molecular mass of 42 kDa, which shows 58% identity to the AC40 subunit shared by RNA polymerases I and III in S. cerevisiae. Furthermore, we have shown that rpa42 + complements a temperature-sensitive mutation in RPC40 the gene that encodes AC40 in S. cerevisiae and which is essential for cell growth. Finally, we have shown that neither RPA42 nor RPA17 can self-associate. These results provide evidence that the two distinct α-related subunits, RPA42 and RPA17, of RNA polymerases I and III are functionally conserved between S. pombe and S. cerevisiae, and suggest that heterodimer formation between them is essential for the assembly of RNA polymerases I and III in eukaryotes.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1433-4909
    Keywords: Key words Extremely barophilic bacteria ; Deep Sea ; Shewanella benthica ; Moritella yayanosii ; Mariana Trench
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have isolated two strains of extremely barophilic bacteria from sediment collected from the world's deepest ocean floor in the Mariana Trench, Challenger Deep, at a depth of 10 898 m [Kato C, Li L, Nogi Y, Nakamura Y, Tamaoka J, Horikoshi K (1998) Appl Environ Microbiol 64:1510-1513]. One strain, DB21MT-2, was identified as a strain of Shewanella benthica, and the other strain, DB21MT-5, is closely affiliated with members of the genus Moritella on the basis of 16S rDNA sequence analysis. The hybridization values for DNA-DNA relatedness between DB21MT-5 and the Moritella reference strains were significantly lower than that accepted as the phylogenetic definition of a species. Based on this and other taxonomic differences, strain DB21MT-5 appears to represent a novel obligately barophilic deep-sea Moritella species. The name Moritella yayanosii (JCM 10263) is proposed. This is the first proposed species of obligately barophilic bacteria of the genus Moritella.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Two dominant uninducible mutant alleles in the gal80 locus were identified. The GAL80 s-1 and GAL80 s-2 mutants showed novel phenotypes in response to the newly isolated GAL81-1 mutant allele, a dominant constitutive mutation linked to the gal4 locus; the GAL80 s-1 GAL81-1 strain was inducible and the GAL80 s-2 GAL81-1 strain was uninducible. Many galactose positive revertants from the GAL80 s-2 GAL81-1 strain were isolated. It was proved that each revertant was due to a secondary mutation either in the gal80 or GAL81 locus, whereas revertants due to mutation at the supposed controlling site for the structural gene cluster of the galactose-pathway enzymes have not been isolated.
    Type of Medium: Electronic Resource
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  • 7
    ISSN: 1617-4623
    Keywords: Key wordsSchizosaccharomyces pombe ; RNA polymerase I ; Interspecific complementation ; α-Subunit ; Deletion analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Eukaryotic RNA polymerases I and III consist of multiple subunits. Each of these enzymes includes two distinct and evolutionarily conserved subunits called α-related subunits which are shared only by polymerases I and III. The α-related subunits show limited homology with the α-subunit of prokaryotic RNA polymerase. To gain further insight into the structure and function of α-related subunits, we cloned and characterized a gene from Schizosaccharomyces pombe that encodes a protein of 17 kDa which can functionally replace AC19 – an α-related subunit of RNA polymerases I and III of Saccharomyces cerevisiae– and was thus named rpa17 +. RPA17 has 125 amino acids and shows 63% identity to AC19 over a 108-residue stretch, whereas the N-terminal regions of the two proteins are highly divergent. Disruption of rpa17 + shows that the gene is essential for cell growth. Sequence comparison with other α-related subunits from different species showed that RPA17 contains an 81-amino acid block that is evolutionarily conserved. Deletion analysis of the N- and C-terminal regions of RPA17 and AC19 confirms that the 81-amino acid block is important for the function of the α-related subunits.
    Type of Medium: Electronic Resource
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