ISSN:
1612-1112
Keywords:
Size exclusion chromatography
;
Multiple detection
;
Mark-Houwink constants
;
Pullulan
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary A size exclusion chromatography system is outlined using multiple detection by a low angle laser light scattering detector (LALLS) and a concentration detector (RI) in conjunction with a differential viscosimeter. The combination of these three detectors parmits the easy and rapid determination of Mw, Mn, Mn and of the Mark-Houwink constants k and α. This system is based on commercially available components and we describe its use with an aqeous solvent system. A similar configuration described in the literature employs a homemade differential viscosimeter constructed by the authors and uses an organic solvent for the SEC. Working with aqeous solvents requires high performance of all equipment components. We will demonstrate the capability of this system with Pullalan as an example.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02316438
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