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  • 1
    Electronic Resource
    Electronic Resource
    Genetic diversity of some surviving on-farm Italian common bean (Phaseolus vulgaris L.) landraces (2005)
    Berlin, Germany : Blackwell Verlag GmbH
    Plant breeding 124 (2005), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: This study was undertaken to estimate the level of variation among and within 33 local populations belonging to seven Italian common bean landraces, by analysing the polymorphism of seed storage proteins, simple sequence repeat (SSR) and amplified fragment length polymorphism (AFLP) molecular markers. The nutritional seed quality of the landraces studied was also investigated. Results showed that the landraces retain a considerable level of heterogeneity. Use of both biochemical and molecular approaches provided a picture of the genetic diversity of each landrace. Cluster analyses based on Nei's genetic distances and Jaccard's similarity index as defined by SSR and AFLP markers, respectively, showed that all populations clustered into two groups corresponding to the Andean and the Mesoamerican gene pools. Knowledge of the genetic structure of a landrace is fundamental in elaborating strategies, which involve the local farmers, allowing us to improve and, at the same time, safeguard the genetic integrity of landrace genetic resources.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0523.2005.01153.x
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    X-ray induction of chromatid interchanges in somatic cells of Drosophila melanogaster: Variations through the cell cycle of the pattern of rejoining
    Amsterdam : Elsevier
    Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis 35 (1976), S. 101-109 
    Details
    ISSN: 0027-5107
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0027-5107(76)90172-X
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Genomic organization and phylogenetic relationships in the genus Dasypyrum analysed by Southern and in situ hybridization of total genomic and cloned DNA probes (1997)
    Springer
    Chromosoma 106 (1997), S. 53-61 
    Details
    ISSN: 1432-0886
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. Molecular cytogenetic methods have been used to study the controversial phylogenetic relationships between the species Dasypyrum villosum (L.) Candargy (2n=2x=14) and D. breviaristatum (Lindb. f.) Frederiksen (2n=4x=28). Using total genomic DNA from the two species as probes for in situ hybridization to chromosomes, we found that the pericentromeric regions of the chromosome arms of both species are similar, while distal regions show substantial differences. Two dispersed repetitive DNA sequences were isolated: pDbKB45 is distributed along the chromosomes but amplified in the subtelomeric regions of D. breviaristatum chromosomes, while pDbKB49, in both species, is less amplified in terminal regions. Size-separated restriction enzyme digests of DNA showed many repetitive fragments, but few in common between the two species. After probing Southern transfers with D. breviaristatum genomic DNA, all lanes showed similar hybridization patterns although one extra small band was evident in the D. breviaristatum lanes. In contrast, probing with D. villosum DNA showed very substantial differences between the two species. Genomic in situ hybridization to meiotic metaphases from an interspecific hybrid showed seven bivalents of D. breviaristatum origin and seven univalents from D. villosum. We also analysed the physical organization of 5S rDNA, 18S-25S rDNA and a tandemly repeated sequence from rye. Our data support an autotetraploid origin for D. breviaristatum, but its genome and that of D. villosum show extensive differences, so the tetraploid is unlikely to be directly derived from D. villosum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004120050224
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    The molecular cytogenetics of Vigna unguiculata (L.) Walp: the physical organization and characterization of 18s-5.8s-25s rRNA genes, 5s rRNA genes, telomere-like sequences, and a family of centromeric repetitive DNA sequences (1995)
    Springer
    Theoretical and applied genetics 91 (1995), S. 928-935 
    Details
    ISSN: 1432-2242
    Keywords: rDNA sites ; Centromeric repetitive DNA ; Telomere ; In situ hybridization ; Southern hybridization ; Ag-NOR ; Cowpea ; Physical maps
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A knowledge of genome organization is important for understanding how genomes function and evolve, and provide information likely to be useful in plant breeding programmes involving hybridization and genetic manipulation. Molecular techniques, including in situ hybridization, molecular cloning and DNA sequencing, are proving valuable tools to investigate the structure, organization, and diversity of chromosomes in agricultural crops. Heterologous labelled 18 s-5.8 s-25 s (pTa71) and 5 s rDNAs (pTa794) were used for in situ hybridization on Vigna unguiculata (L.) Walp. chromosomes. Hybridization with 18 s-5.8 s-25 s rRNA gene probes occurred at the same chromosomal sites which were positive to the CMA fluorochrome. Silver staining of nucleolar-organizing regions indicated that all the rDNA sites detected using the 18 s-5.8 s-25 s rRNA gene probe possessed active genes. Degenerate telomeric repeats gave hybridization signals at the telomeres of most chromosomes and no intercalary sites were detected at metaphase; the sequences appear to have no preferential distribution in interphase nuclei. A repetitive DraI family from V. unguiculata was cloned (pVuKB1) and characterized. The DraI repeat is 488 nucleotides long, AT rich (74%), and hybridized on all chromosomes in the centromeric areas. The presence of this sequence family was investigated by Southern hybridization in different Vigna species and other Leguminoseae. It was only detected in V. unguiculata, and hence represents a species-specific DNA sequence.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00223902
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  • 5
    Electronic Resource
    Electronic Resource
    The biochemical and cytological characterization of Vicia faba DNA by means of MboI, AluI and Bam HI restriction endonucleases (1987)
    Springer
    Theoretical and applied genetics 75 (1987), S. 46-50 
    Details
    ISSN: 1432-2242
    Keywords: Vicia faba ; Restriction endonucleases ; Electrophoretic pattern ; Chromosome banding pattern ; Cleavage of DNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Restriction endonucleases were employed to characterize both cytologically and electrophoretically the DNA of Vicia faba. The electrophoretic pattern of total DNA digested with AluI and MboI shows a continuous smear. Bam HI also shows a continuous smear for the bigger polynucleotide fragments and several bands in the lower part of the lane. Digestion of fixed chromosomal DNA produces metaphase longitudinal differentiation when MboI and AluI are used, while no appreciable banding pattern is present when Bam HI is employed. These results are discussed in relation to the organization of chromosomal DNA, to other data in the literature on chromosome banding and on the digestion of total DNA of other species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00249141
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