ISSN:
0138-4988
Keywords:
Life Sciences
;
Life Sciences (general)
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Process Engineering, Biotechnology, Nutrition Technology
Notes:
The stability of the plasmid pBR 322 was measured in E. coli NF 161 (rel A+) and NF 162 (rel A) grown in a methionine limited chemostat. A significant plasmid loss occurred only in E. coli NF 162 at a low dilution rate and if the fermenter had been inoculated with cells from the stationary phase. Obviously, this behaviour resulted from the high expression of β-lactamase due to amplification of the pBR 322 DNA in the inoculum cells of E. coli NF162.
Additional Material:
2 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/abio.370110420
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