ISSN:
1432-2307
Keywords:
Interferon-γ
;
Tumour necrosis factor-α
;
Transforming growth factor-β
;
Interleukin-1β
;
Inflammation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract The expression of the β1 integrins was examined immunohistochemically in synoviocytes from normal synovial membrane and from chronic synovitis of different aetiology and intensity. Normal synoviocytes were α6β1-positive but lacked α1 through α5. In mild inflammation type A synoviocytes neo-expressed α1, α3, and α5 chains. In severe inflammation both type A and B synoviocytes expressed α3, α4, α5, and α6 chains. The effects of inflammatory cytokines, as single agents or in combination, on the β1 integrin expression in cultured normal synoviocytes was determined by immunocytochemistry and flow cytometry. The α1 chain, while absent in unstimulated synoviocytes, was induced by interleukin-1β (IL-1β), tumour necrosis factor-α (TNF-α), and interferon-γ (INF-γ). This effect was enhanced by combining IL-1β and TNF-α. Expression of the α3 chain was up-regulated by IL-1β and, more intensely, by IFN-γ. Transforming growth factor β (TGF-β) inhibited the up-regulating effect of IL-1β and antagonized the effect of IFN-γ on α3 chain expression. Expression of the α5 chain was up-regulated significantly by co-stimulation through IL-1β together with TGF-β or TNF-α. Thus, the β1 integrin profile of cytokine activated synoviocytes in vitro resembled that of synoviocytes in synovitis in situ. These data suggest that IL-1β, TNF-α, IFN-γ, and TGF-β are likely to be among the effectors regulating β1 integrin expression in synoviocytes in vivo.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00230354
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